Electrospun fish gelatin fibrous scaffolds with improved bio-interactions due to carboxylated nanodiamond loading

Serafim, Andrada; Cecoltan, Sergiu; Lungu, Adriana; Vasile, Eugeniu; Iovu, Horia; Stancu, Izabela‐Cristina

doi:10.1039/c5ra14361f

Cited by 20 publications

(26 citation statements)

References 47 publications

(66 reference statements)

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“…This can explain the enhanced seeding efficiency of BMSCs in poly(LLA‐ co ‐CL)/nDPs scaffolds. This findings is supported by recent studies, e.g., the addition of more nDPs to gelatin fibrous scaffolds improved the number of adherent cells, their morphology, and spreading . nDPs are also reported to enhance cell adhesion, proliferation, and stimulate mineralization .…”

Section: Discussionsupporting

confidence: 81%

“…This findings is supported by recent studies, e.g., the addition of more nDPs to gelatin fibrous scaffolds improved the number of adherent cells, their morphology, and spreading. [61] nDPs are also reported to enhance cell adhesion, proliferation, and stimulate mineralization. [61,62] Coating the polymer with nDPs produces a surface with multiple binding sites for adhesion of molecules, which is beneficial for cell adhesion and growth.…”

Section: Discussionmentioning

confidence: 99%

“…[61] nDPs are also reported to enhance cell adhesion, proliferation, and stimulate mineralization. [61,62] Coating the polymer with nDPs produces a surface with multiple binding sites for adhesion of molecules, which is beneficial for cell adhesion and growth.…”

Section: Discussionmentioning

confidence: 99%

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A Copolymer Scaffold Functionalized with Nanodiamond Particles Enhances Osteogenic Metabolic Activity and Bone Regeneration

Yassin

Mustafa

Xing

et al. 2017

Macromolecular Bioscience

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Functionalizing polymer scaffolds with nanodiamond particles (nDPs) has pronounced effect on the surface properties, such as improved wettability, an increased active area and binding sites for cellular attachment and adhesion, and increased ability to immobilize biomolecules by physical adsorption. This study aims to evaluate the effect of poly(l-lactide-co-ε-caprolactone) (poly(LLA-co-CL)) scaffolds, functionalized with nDPs, on bone regeneration in a rat calvarial critical size defect. Poly(LLA-co-CL) scaffolds functionalized with nDPs are also compared with pristine scaffolds with reference to albumin adsorption and seeding efficiency of bone marrow stromal cells (BMSCs). Compared with pristine scaffolds, the experimental scaffolds exhibit a reduction in albumin adsorption and a significant increase in the seeding efficiency of BMSCs (p = 0.027). In the calvarial defects implanted with BMSC-seeded poly(LLA-co-CL)/nDPs scaffolds, live imaging at 12 weeks discloses a significant increase in osteogenic metabolic activity (p = 0.016). Microcomputed tomography, confirmed by histological data, reveals a substantial increase in bone volume (p = 0.021). The results show that compared with conventional poly(LLA-co-CL) scaffolds those functionalized with nDPs promote osteogenic metabolic activity and mineralization capacity. It is concluded that poly(LLA-co-CL) composite matrices functionalized with nDPs enhance osteoconductivity and therefore warrant further study as potential scaffolding material for bone tissue engineering.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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A Copolymer Scaffold Functionalized with Nanodiamond Particles Enhances Osteogenic Metabolic Activity and Bone Regeneration

Yassin

Mustafa

Xing

et al. 2017

Macromolecular Bioscience

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“…Cell culture Both gelatin and GelMA are well investigated materials for tissue engineering applications and were often used in order to induce cell adherence in various systems [3,6,10,11,25,27,28]. The biocompatibility of the synthesized materials was evaluated in terms of cell viability using freeze-dried scaffolds.…”

Section: Drug Release Profilementioning

confidence: 99%

Bicomponent Hydrogels Based on Methacryloyl Derivatives of Gelatin and Mucin with Potential Wound Dressing Applications

Serafim¹,

Olăreţ²,

Cecoltan³

et al. 2018

Mat.Plast.

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The present paper reports the first attempt to synthesize bicomponent hydrogels based on methacryloyl derivatives of gelatin (GelMA) and mucin (MuMA) with different compositions, with potential as wound dressings. While gelatin is widely investigated and used to fabricate scaffolds and coatings stimulating cell interactions and tissue regeneration, mucin - a macromolecule which covers the wet epithelia - remains yet under exploited as biomaterial. The influence of MuMA content on various parameters such as the affinity for aqueous media, stability in simulated physiologic media and the rheologic behavior was investigated. Also, the preliminary assessment of the drug release potential and biocompatibility were performed. The materials� water uptake capacity and rheologic behavior depend on the pH value of the incubation media, while their composition influences the drug release capacity and cells-scaffold interactions.

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“…Gelatin, a naturally derived biopolymer from collagen, has been extensively proposed due to its arginine–glycine–aspartic acid (RGD) sequence, biocompatibility, biodegradability, and variety from multiple sources [ 8 , 12 , 14 , 17 , 18 ]. While ink formulations are typically prepared with mammalian gelatins, religious issues or the risk of bovine spongiform encephalopathy transmitting may bring to attention the use of other types of gelatin [ 19 , 20 , 21 ]. Fish gelatin (FG) can be chosen as an interesting option for biomedical applications, as we previously reported for injectable solutions for electrospinning [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Development of 3D Bioactive Scaffolds through 3D Printing Using Wollastonite–Gelatin Inks

et al. 2020

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The bioactivity of scaffolds represents a key property to facilitate the bone repair after orthopedic trauma. This study reports the development of biomimetic paste-type inks based on wollastonite (CS) and fish gelatin (FG) in a mass ratio similar to natural bone, as an appealing strategy to promote the mineralization during scaffold incubation in simulated body fluid (SBF). High-resolution 3D scaffolds were fabricated through 3D printing, and the homogeneous distribution of CS in the protein matrix was revealed by scanning electron microscopy/energy-dispersive X-ray diffraction analysis (SEM/EDX) micrographs. The bioactivity of the scaffold was suggested by an outstanding mineralization capacity revealed by the apatite layers deposited on the scaffold surface after immersion in SBF. The biocompatibility was demonstrated by cell proliferation established by MTT assay and fluorescence microscopy images and confirmed by SEM micrographs illustrating cell spreading. This work highlights the potential of the bicomponent inks to fabricate 3D bioactive scaffolds and predicts the osteogenic properties for bone regeneration applications.

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Electrospun fish gelatin fibrous scaffolds with improved bio-interactions due to carboxylated nanodiamond loading

Abstract: This work emphasizes the potential of COOH-functionalized nanodiamond (NDs) particles to improve bio-interactions when embedded into fish gelatin electrospun fibers.

Cited by 20 publications

References 47 publications

A Copolymer Scaffold Functionalized with Nanodiamond Particles Enhances Osteogenic Metabolic Activity and Bone Regeneration

A Copolymer Scaffold Functionalized with Nanodiamond Particles Enhances Osteogenic Metabolic Activity and Bone Regeneration

Bicomponent Hydrogels Based on Methacryloyl Derivatives of Gelatin and Mucin with Potential Wound Dressing Applications

Development of 3D Bioactive Scaffolds through 3D Printing Using Wollastonite–Gelatin Inks

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