Bicomponent Hydrogels Based on Methacryloyl Derivatives of Gelatin and Mucin with Potential Wound Dressing Applications

Serafim, Andrada; Olăreţ, Elena; Cecoltan, Sergiu; Butac, Livia Maria; Bãlãnucã, Brîndușa; Vasile, Eugeniu; Ghica, Mihaela Violeta; Stancu, Izabela‐Cristina

doi:10.37358/mp.18.1.4965

Cited by 9 publications

(14 citation statements)

References 19 publications

(28 reference statements)

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“…4F), while lidocaine was shown to be released over hours from mixed bovine submaxillary mucin (BSM)-gelatin hydrogels. 92 The sustained release of the drugs could be attributed to interactions with mucins, since the high molar mass but poorly mucoadhesive molecules released faster than the two small model drugs. The hydrophilicity and negative charge of mucins were also used by Nowald et al who mixed pig gastric mucins with methylcellulose to create thermo-sensitive selfstanding hydrogels that bound to positively charged molecules.…”

Section: Exploiting the Complex Chemistry Of Mucinsmentioning

confidence: 99%

Mucins as multifunctional building blocks of biomaterials

2018

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Mucins are large glycoproteins that are ubiquitous in the animal kingdom. Mucins coat the surfaces of many cell types and can be secreted to form mucus gels that assume important physiological roles in many animals. Our growing understanding of the structure and function of mucin molecules and their functionalities has sparked interest in investigating the use of mucins as building blocks for innovative functional biomaterials. These pioneering studies have explored how new biomaterials can benefit from the barrier properties, hydration and lubrication properties, unique chemical diversity, and bioactivities of mucins. Owing to their multifunctionality, mucins have been used in a wide variety of applications, including as antifouling coatings, as selective filters, and artificial tears and saliva, as basis for cosmetics, as drug delivery materials, and as natural detergents. In this review, we summarize the current knowledge regarding key mucin properties and survey how they have been put to use. We offer a vision for how mucins could be used in the near future and what challenges await the field before biomaterials made of mucins and mucin-mimics can be translated into commercial products.

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Section: Exploiting the Complex Chemistry Of Mucinsmentioning

confidence: 99%

Mucins as multifunctional building blocks of biomaterials

2018

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“…Methacryloyl derivatives of proteins, and particularly methacryloyl functionalized gelatins, are increasingly used in regenerative medicine and tissue engineering applications due to their ability to be further polymerized while maintaining the biocompatibility of the pristine protein. Our group has previously used methacryloyl gelatin (GelMA) to improve cell interactivity of synthetic polymers for tissue regeneration [ 15 , 16 ], while methacryloyl mucin (MuMA) also leads to stable hydrogels and has been investigated for controlled release of active molecules [ 17 , 18 ]. Based on our previous experience in preparing GelMA and MuMA scaffolds, we believe that they also have potential for reconstructive medicine; therefore, in this study, our aim was to engineer ECM-bioinspired coatings for PP meshes and further explore their potential in improving the efficiency of PRP in promoting fibroblasts interactions.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Hydrogel Coating Based on Methacryloyl Gelatin Bioactivates Polypropylene Meshes for Abdominal Wall Repair

et al. 2020

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Considering the potential of hydrogels to mimic the cellular microenvironment, methacryloyl gelatin (GelMA) and methacryloyl mucin (MuMA) were selected and compared as bioinspired coatings for commercially available polypropylene (PP) meshes for ventral hernia repair. Thin, elastic hydrated hydrogel layers were obtained through network-forming photo-polymerization, after immobilization of derivatives on the surface of the PP fibers. Fourier transform infrared spectroscopy (FTIR) proved the successful coating while the surface morphology and homogeneity were investigated by scanning electron microscopy (SEM) and micro-computed tomography (micro-CT). The stability of the hydrogel layers was evaluated through biodynamic tests performed on the coated meshes for seven days, followed by inspection of surface morphology through SEM and micro-CT. Taking into account that platelet-rich plasma (PRP) may improve healing due to its high concentration of growth factors, this extract was used as pre-treatment for the hydrogel coating to additionally stimulate cell interactions. The performed advanced characterization proved that GelMA and MuMA coatings can modulate fibroblasts response on PP meshes, either as such or supplemented with PRP extract as a blood-derived bioactivator. GelMA supported the best cellular response. These findings may extend the applicative potential of functionalized gelatin opening a new path on the research and engineering of a new generation of bioactive meshes.

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“…Gastric mucin, for example, aggregates and forms stable gels at low pH values, thus preventing the stomach from digesting itself . Exploiting its pH dependency and versatile structure, mucin has been studied for possible applications in the biomedical field as lubricant of various surfaces, barrier against pathogens, drug delivery vehicle, etc.…”

Section: Introductionmentioning

confidence: 99%

Coatings based on mucin‐tannic acid assembled multilayers. Influence of pH

Olăreţ

Ghițman

Iovu

et al. 2020

Polymers for Advanced Techs

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Although widely used as implantable materials due to their mechanical properties, metal devices show several disadvantages, such as the lack of cellular binding sites, antibacterial properties, or lubricant properties. To this end, the development of engineered surfaces through protein coatings has been largely investigated. Due to their natural origin and complex structure which allows the formation of hydrogels, mucins have been proposed and investigated as effective coatings for metallic implants. The present study evaluates the ability of porcine gastric mucin (PGM) to form stable coatings on a model metal surface, using three buffers with different pH values, ranging from acid to alkaline, as dispersion media. Considering its large number of hydroxyl groups and its ability to form hydrogen bonds with the protein, tannic acid (TA) was used as cross‐linker. In addition to coatings' thickness and elasticity assessed through Quartz Crystal Microbalance with Dissipation monitoring (QCM‐D), the study also evaluates the interactions between PGM and TA at various pH values of the dispersion media through DLS. The corroborated results show that the neutral pH allows the best interactions between PGM and TA leading to the formation of a stable, less elastic coating when compared with that obtained using as dispersion media the other two investigated buffers. Moreover, the hydrophilicity and mechanical properties of the dried coatings were assessed through contact angle measurements and nanoindentation, respectively.

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Bicomponent Hydrogels Based on Methacryloyl Derivatives of Gelatin and Mucin with Potential Wound Dressing Applications

Cited by 9 publications

References 19 publications

Mucins as multifunctional building blocks of biomaterials

Mucins as multifunctional building blocks of biomaterials

Bioinspired Hydrogel Coating Based on Methacryloyl Gelatin Bioactivates Polypropylene Meshes for Abdominal Wall Repair

Coatings based on mucin‐tannic acid assembled multilayers. Influence of pH

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