Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids

Coghetto, Chaline Caren; Brinques, Graziela Brusch; Siqueira, Nataly Machado; Pletsch, Jéssica; Soares, Rosane Michele Duarte; Ayub, Marco Antônio Záchia

doi:10.1016/j.jff.2016.03.036

Cited by 85 publications

(38 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The diameter value varied from 0.4 up to 4.9 mm with an average diameter of 1.4 ± 1 mm. These sizes are in the range reported for electro sprayed alginate microcapsules containing Lactobacillus plantarum and for other particles generated by different encapsulation methods (emulsion, extrusion, and spray drying) …”

Section: Resultsmentioning

confidence: 56%

“…In this method, the size of particles formed can be controlled by changes in the flow rates of the working fluids and the applied voltage . The electro spraying in protein‐based drugs and cells have demonstrated the applicability of this method in drug delivery and controlled released systems . Tetraaxial electro spraying is a customized version where a tetraaxial needle is used.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrospray‐assisted fabrication of core‐shell arabinoxylan gel particles for insulin and probiotics entrapment

Paz-Samaniego

Rascón‐Chu²,

Brown

et al. 2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

This work presents the fabrication and characterization of electro-sprayed core-shell particles that were composed of maize bran arabinoxylans (MBAX) with insulin in the core, and maize wastewater arabinoxylans (MWAX) with Bifidobacterium in the shell. Two concentrations of MBAX (3% and 6% w/v) and MWAX (6% and 10% w/v) were evaluated. The particles fabricated with MBAX at 6% (w/v) in the core and MWAX at 10% (w/v) in the shell were more stable, presented spherical shape and no aggregation being therefore selected to be loaded with insulin and probiotics. These particles presented a size of 2.9 mm. Scanning electron microscopy analysis of the particle cross section revealed the presence of both, a smooth (shell) and a porous (core) microstructure. Confocal laser scanning microscopy confirmed the core-shell structure of the particles and the viability of the probiotic entrapped. Gastrointestinal simulation strongly suggests that these particles are not degraded in the stomach and small intestine and that 76% of the carried insulin is released in colon. These results indicate that insulin and Bifidobacterium encapsulation by tetraaxial electro spraying can be a feasible and adequate technique to produce arabinoxylan capsules containing both insulin and probiotics. V C 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46411.

show abstract

Section: Resultsmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Electrospray‐assisted fabrication of core‐shell arabinoxylan gel particles for insulin and probiotics entrapment

Paz-Samaniego

Rascón‐Chu²,

Brown

et al. 2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…The system of electrospraying potentially has some advantageous such as controlling the morphology and the size distribution of droplets in order to fabricate nearly monodisperse particles in an economic and one-step manner at ambient pressure and temperature [13]. The electrospraying method has been successfully used to fabricate submicron polymerbased structures such as thin film deposition [14], capsule formation [15] and particle production [16]. The technique has various applications in microelectronics [17], optical electronics, biotechnology, environmental engineering [18], modern material and medical technologies, new DDSs and ingredients dosage fabrication in the food, cosmetic and medicine production industries [19].…”

Section: Introductionmentioning

confidence: 99%

Morphological and physicochemical evaluation of the propranolol HCl–Eudragit^® RS100 electrosprayed nanoformulations

Garjani

Barzegar-Jalali

Osouli-Bostanabad

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

The aim of this study was to fabricate propranolol hydrochloride (Prop. HCl) (as a water-soluble drug):Eudragit RS100 (Eud) nanobeads and nanofibres applying the electrospraying method as an economical and one-step technique. Different ratios of Prop. HCl:Eud (i.e. 1:5 and 1:10) at total solution concentrations of 10-20% W/V were investigated. The FE-SEM studies revealed that morphology and size of the samples were highly affected by the solution concentration; so that, the nanobeads (a mean diameter of 82.9 nm) were formed in low concentration and at the highest concentration of the solution, nanofibres (a mean diameter of 232.3 nm) were resulted. Besides the morphological changes, the size of processed nanoformulations was increased with an increment of the solution concentrations. X-ray diffraction results as well as DSC thermograms clearly indicated that the drug crystallinity decreased in the electrosprayed samples. Furthermore, in vitro dissolution test showed that the electrosprayed samples had relatively slower release patterns toward the pure drug and physical mixtures, where the samples with the drug:polymer ratio of 1:10 indicated a faster release rate toward 1:5 ratio; nevertheless, the concentration of the injected formulations did not remarkably impressed the release behaviours. The current study established the suitability of electrospraying method in the fabrication of the water-soluble drugs nanobeads/nanofibres; however, in vivo effectiveness of the prepared nanoformulations should be meticulously considered.

show abstract

“…Electrosprayed WPC-based capsules were also used to protect a strain of Lactobacillus plantarum (CECT 748 T), which experienced a reduced viability loss during in vitro digestion (∼3 log CFU g −1 ) (Gomez-Mascaraque et al, 2016). In contrast, alginateencapsulated L. plantarum (strain UFRGS BL011) suffered a viability loss of almost 3 log CFU mL −1 when exposed to simulated gastric juice, and an additional drop of 3 log CFU mL −1 when exposed to simulated intestinal juice (Coghetto et al, 2016). However, these results are not directly comparable, since the protected L. plantarum strain is different and the protocols employed for simulation of gastrointestinal conditions also differ.…”

Section: Encapsulation Of Probioticsmentioning

confidence: 99%

Nanostructuring Biopolymers for Improved Food Quality and Safety

Gómez‐Mascaraque

Fabra

Castro-Mayorga

et al. 2018

Biopolymers for Food Design

View full text Add to dashboard Cite

Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids

Cited by 85 publications

References 63 publications

Electrospray‐assisted fabrication of core‐shell arabinoxylan gel particles for insulin and probiotics entrapment

Electrospray‐assisted fabrication of core‐shell arabinoxylan gel particles for insulin and probiotics entrapment

Morphological and physicochemical evaluation of the propranolol HCl–Eudragit^® RS100 electrosprayed nanoformulations

Nanostructuring Biopolymers for Improved Food Quality and Safety

Contact Info

Product

Resources

About

Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids

Cited by 85 publications

References 63 publications

Electrospray‐assisted fabrication of core‐shell arabinoxylan gel particles for insulin and probiotics entrapment

Electrospray‐assisted fabrication of core‐shell arabinoxylan gel particles for insulin and probiotics entrapment

Morphological and physicochemical evaluation of the propranolol HCl–Eudragit® RS100 electrosprayed nanoformulations

Nanostructuring Biopolymers for Improved Food Quality and Safety

Contact Info

Product

Resources

About

Morphological and physicochemical evaluation of the propranolol HCl–Eudragit^® RS100 electrosprayed nanoformulations