Electrospray ionization and matrix‐assisted laser desorption/ionization mass spectrometric analysis of extrapolysaccharides of <i>Escherichia coli</i>

Gedda, Gangaraju; Gopal, Judy; Wu, Hui‐Fen

doi:10.1002/rcm.6263

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…A common feature between positive and negative spectra is represented by the occurrence of very intense ions at m / z 1293.59 (positive) and m / z 1291.59 (negative). This peak could be probably ascribed to the class of membrane extrapolysaccharides which usually protect bacteria from predators and environmental stresses (certain attribution needs of course further investigations by tandem MS analysis). Contrary to these findings, investigations carried out on lipids extracted from E .…”

Section: Resultsmentioning

confidence: 99%

MALDI‐TOF mass spectrometry analysis of proteins and lipids in Escherichia coli exposed to copper ions and nanoparticles

et al. 2016

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Escherichia coli (E. coli) is one of the most important foodborne pathogens to the food industry responsible for diseases as bloody diarrhea, hemorrhagic colitis and life-threatening hemolytic-uremic syndrome. For controlling and eliminating E. coli, metal nano-antimicrobials (NAMs) are frequently used as bioactive systems for applications in food treatments. Most NAMs provide controlled release of metal ions, eventually slowing down or completely inhibiting the growth of undesired microorganisms. Nonetheless, their antimicrobial action is not totally unraveled and is strongly dependent on metal properties and environmental conditions. In this work, we propose the use of matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry as a powerful tool for direct, time efficient, plausible identification of the cell membrane damage in bacterial strains exposed to copper-based antimicrobial agents, such as soluble salts (chosen as simplified AM material) and copper nanoparticles. E. coli ATCC 25922 strain was selected as 'training bacterium' to set up some critical experimental parameters (i.e. cell concentration, selection of the MALDI matrix, optimal solvent composition, sample preparation method) for the MS analyses. The resulting procedure was then used to attain both protein and lipid fingerprints from E. coli after exposure to different loadings of Cu salts and NPs. Interestingly, bacteria exposed to copper showed over-expression of copper binding proteins and degradation of lipids when treated with soluble salt. These findings were completed with other investigations, such as microbiological experiments. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

MALDI‐TOF mass spectrometry analysis of proteins and lipids in Escherichia coli exposed to copper ions and nanoparticles

et al. 2016

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“…Chemical extraction methods were found to be more efficient than the physical extraction methods and of the chemical extractants, EDTA was found more effective than formaldehyde and gave more polysaccharide peaks using both ESI‐ and MALDI‐TOF analysis (using CHCA as the matrix). Detection by ESI‐MS was very effective for compounds <300 Da whereas MALDI‐TOF MS was found to be more suitable for rapid analysis of the polysaccharides above 400 Da (Gedda et al, ).…”

Section: Studies On Specific Carbohydrate Typesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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“…For instance, CdS@MPA causes quantitative degradation of extracellular polysaccharides (EPS) of Escherichia coli. 69 To investigate the cell viability of CdS@CTS against the two pathogenic bacteria, the optical density method was used and the optical density (OD 600 ) was measured at 600 nm. The method is based on the turbidity measurements which give signals corresponding to the bacteria cell numbers.…”

Section: Stability Of the Cds@cts Qdsmentioning

confidence: 99%

Probing the interactions of chitosan capped CdS quantum dots with pathogenic bacteria and their biosensing application

Abdelhamid

2013

J. Mater. Chem. B

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116

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Electrospray ionization and matrix‐assisted laser desorption/ionization mass spectrometric analysis of extrapolysaccharides of Escherichia coli

Abstract: This is a preliminary study reporting for the first time the feasibility of ESI-MS and MALDI-MS techniques for the analysis of bacterial EPS without prior complicated purification and separation methodologies.

Cited by 4 publications

References 21 publications

MALDI‐TOF mass spectrometry analysis of proteins and lipids in Escherichia coli exposed to copper ions and nanoparticles

MALDI‐TOF mass spectrometry analysis of proteins and lipids in Escherichia coli exposed to copper ions and nanoparticles

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Probing the interactions of chitosan capped CdS quantum dots with pathogenic bacteria and their biosensing application

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