Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification

Abstract

The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137–141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the α137–141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase—75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the α137–141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF α137–141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

“…The fraction was obtained at the lowest energy consumption (3.4–5 kWh/kg) when using 5–8% hemoglobin in the initial feed. The good antimicrobial results led them to optimize the process by studying the influence of the degree of hydrolysis (DH) [ 338 ] and the pH [ 339 ]. By targeting TSKYR (α 137–141, 653 Da), an antimicrobial peptide with interesting properties for meat taste preservation, they found that pH 9 increased the purity of the fraction, while a 5% DH allowed a 13-fold concentration of TSKYR compared to the initial hydrolysate.…”

Recovery of protein‐based compounds from meat by‐products by membrane‐assisted separations: a review

“…Using electrolysis aided membrane technology, Przybylski et al 111 recovered a peptide fraction containing more than 50% of the ⊍137-141 peptide from slaughterhouse by-product hydrolysate. Such a bioactive peptide has antibacterial activity against several pathogenic bacteria that cause food spoilage.…”

“…Using electrolysis aided membrane technology, Przybylski et al 111 . recovered a peptide fraction containing more than 50% of the α137‐141 peptide from slaughterhouse by‐product hydrolysate.…”

Applications of Ultrafiltration, Reverse Osmosis, Nanofiltration, and Microfiltration in Dairy and Food Industry

“…The resultant peptide concentration had 50% of α137-141 in it. Have this peptide concentrate can also be used as a natural preservative alternatively do synthetic additives (Przybylski et al, 2020).…”