Electrorotation Rates of K562 Cells Accompanied by Erythroid Differentiation Induced by Sodium Butyrate

Takeuchi, Rino; Suzuki, Masato; Yasukawa, Tomoyuki

doi:10.2116/analsci.20c017

Cited by 3 publications

(1 citation statement)

References 24 publications

(22 reference statements)

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“…In this way, the measurement of ROT can distinguish the ultrastructure of the surface of the cell membrane via the differences in the rotation rate. Further, ROT has been employed to identify the cell cycle, 17 detect the activation of osmotic-sensitive channels, 18,19 detect human neutrophil activation by the chemotactic factor, 20 distinguish the differentiation state for erythroleukemia cells, 21,22 distinguish cancer cells exhibiting drug resistivity via the overexpression of glycoproteins, 23 obtain the precise 3D cell image, 24,25 and determine three biophysical parameters (shear modulus, steady-state viscosity, and relaxation time from the stretching deformation) by combination with the optical stretching method. 26 ROT has been traditionally performed with four planer square electrodes that were arranged symmetrically around a point.…”

Section: Introductionmentioning

confidence: 99%

Development of a simultaneous electrorotation device with microwells for monitoring the rotation rates of multiple single cells upon chemical stimulation

et al. 2023

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Section: Introductionmentioning

confidence: 99%

Development of a simultaneous electrorotation device with microwells for monitoring the rotation rates of multiple single cells upon chemical stimulation

et al. 2023

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Selective Trapping and Retrieval of Single Cells Using Microwell Array Devices Combined with Dielectrophoresis

2021

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Fabrication of Two-Layer Microfluidic Devices with Porous Electrodes Using Printed Sacrificial Layers

Ino,

Konno,

Utagawa

et al. 2024

Micromachines

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Two-layer microfluidic devices with porous membranes have been widely used in bioapplications such as microphysiological systems (MPS). Porous electrodes, instead of membranes, have recently been incorporated into devices for electrochemical cell analysis. Generally, microfluidic channels are prepared using soft lithography and assembled into two-layer microfluidic devices. In addition to soft lithography, three-dimensional (3D) printing has been widely used for the direct fabrication of microfluidic devices because of its high flexibility. However, this technique has not yet been applied to the fabrication of two-layer microfluidic devices with porous electrodes. This paper proposes a novel fabrication process for this type of device. In brief, Pluronic F-127 ink was three-dimensionally printed in the form of sacrificial layers. A porous Au electrode, fabricated by sputtering Au on track-etched polyethylene terephthalate membranes, was placed between the top and bottom sacrificial layers. After covering with polydimethylsiloxane, the sacrificial layers were removed by flushing with a cold solution. To the best of our knowledge, this is the first report on the sacrificial approach-based fabrication of two-layer microfluidic devices with a porous electrode. Furthermore, the device was used for electrochemical assays of serotonin and could successfully measure concentrations up to 5 µM. In the future, this device can be used for MPS applications.

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Electrorotation Rates of K562 Cells Accompanied by Erythroid Differentiation Induced by Sodium Butyrate

Cited by 3 publications

References 24 publications

Development of a simultaneous electrorotation device with microwells for monitoring the rotation rates of multiple single cells upon chemical stimulation

Development of a simultaneous electrorotation device with microwells for monitoring the rotation rates of multiple single cells upon chemical stimulation

Selective Trapping and Retrieval of Single Cells Using Microwell Array Devices Combined with Dielectrophoresis

Fabrication of Two-Layer Microfluidic Devices with Porous Electrodes Using Printed Sacrificial Layers

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