Selective Trapping and Retrieval of Single Cells Using Microwell Array Devices Combined with Dielectrophoresis

Hata, Miasaki; Suzuki, Masato; Yasukawa, Tomoyuki

doi:10.2116/analsci.21c002

Cited by 7 publications

(1 citation statement)

References 32 publications

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“…The size of the microfluidic channel is close to that of a single cell, which makes it especially suitable for manipulating/analyzing single cell [7][8][9]. Typical microfluidic chips for single-cell separation use hydrodynamics [10], dielectrophoresis (DEP) [11][12][13][14], microwell array [15,16], droplets [17], and microvalves [18,19]. Among them, the droplets-based single-cell analysis enables high-throughput single-cell sorting and analysis, but the method of encapsulating individual cells with droplets is uneven [17].…”

Section: Introductionmentioning

confidence: 99%

Trapping and releasing of single microparticles and cells in a microfluidic chip

Zhang

et al. 2022

Electrophoresis

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A microfluidic device was designed and fabricated to capture single microparticles and cells by using hydrodynamic force and selectively release the microparticles and cells of interest via negative dielectrophoresis by activating selected individual microelectrodes. The trap microstructure was optimized based on numerical simulation of the electric field as well as the flow field. The capture and selective release functions of the device were verified by multi-types microparticles with different diameters and K562 cells. The capture efficiencies/release efficiencies were 95.55% ± 0.43%/96.41% ± 1.08% and 91.34% ± 0.01%/93.67% ± 0.36% for microparticles and cells, respectively. By including more traps and microelectrodes, the device can achieve high throughput and realize the visual separation of microparticles/cells of interest in a large number of particle/cell groups.

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Section: Introductionmentioning

confidence: 99%

Trapping and releasing of single microparticles and cells in a microfluidic chip

Zhang

et al. 2022

Electrophoresis

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Harnessing the Manipulation of Single Cells to Construct Biological Structures: Tools and Applications

Liu,

Chen,

Tong

et al. 2024

Adv Funct Materials

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Artificial biological structures hold the promise for modeling cellular assembly in vitro and have advanced considerable studies in cell biology, disease modeling, drug testing, and regenerative medicine. Biological functions are derived from micro‐ and macroscale interactions of various cell types, and a structural property matching the tissue in vivo is required to enable precision biological function. Despite various types of tissues and organs are successfully constructed by conventional biofabrication technologies, they mostly only show a small fraction of structural features found in real tissues. Tools for single‐cell manipulation provide the approach to fabricate artificial tissues cell‐by‐cell, and have enabled the construction of biological structures with single‐cell and heterogeneous features, recapitulating the complexity in vivo. This review presents a comprehensive overview of the construction of biological structures through manipulating single cells, covering single‐cell technologies with operation principles and main advances, biological structures associated with informative explanations of single‐cell manipulation during construction, and representative applications mainly focusing on analysis and modeling. Current challenges and future perspectives in this field are also discussed.

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Dielectrophoresis: Measurement technologies and auxiliary sensing applications

Hu,

Ji,

Chen

et al. 2024

Electrophoresis

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Dielectrophoresis (DEP), which arises from the interaction between dielectric particles and an aqueous solution in a nonuniform electric field, contributes to the manipulation of nano and microparticles in many fields, including colloid physics, analytical chemistry, molecular biology, clinical medicine, and pharmaceutics. The measurement of the DEP force could provide a more complete solution for verifying current classical DEP theories. This review reports various imaging, fluidic, optical, and mechanical approaches for measuring the DEP forces at different amplitudes and frequencies. The integration of DEP technology into sensors enables fast response, high sensitivity, precise discrimination, and label‐free detection of proteins, bacteria, colloidal particles, and cells. Therefore, this review provides an in‐depth overview of DEP‐based fabrication and measurements. Depending on the measurement requirements, DEP manipulation can be classified into assistance and integration approaches to improve sensor performance. To this end, an overview is dedicated to developing the concept of trapping‐on‐sensing, improving its structure and performance, and realizing fully DEP‐assisted lab‐on‐a‐chip systems.

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Selective Trapping and Retrieval of Single Cells Using Microwell Array Devices Combined with Dielectrophoresis

Abstract: Reasons for UrgentPublication  Rapid formation of cell arrays based in p-DEP  Selective retrieval of the target cells from cell arrays  Selective retention of the target cells by removing undesired cells from cell arrays

Cited by 7 publications

References 32 publications

Trapping and releasing of single microparticles and cells in a microfluidic chip

Trapping and releasing of single microparticles and cells in a microfluidic chip

Harnessing the Manipulation of Single Cells to Construct Biological Structures: Tools and Applications

Dielectrophoresis: Measurement technologies and auxiliary sensing applications

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