Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro

Reberšek, Matej; Faurie, Cécile; Kandušer, Maša; Čorović, Selma; Teissié, Justin; Rols, Marie‐Pierre; Miklavčič, Damijan

doi:10.1186/1475-925x-6-25

Cited by 63 publications

(57 citation statements)

References 35 publications

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“…Such protocols were successfully used for electrochemotherapy and gene electrotransfer (Rols, Teissié 1990a;Tekle et al 1991;Serša et al 1996;Vernhes et al 1999;Kotnik et al 2001a;Kotnik et al 2001b;Golzio et al 2002;Faurie et al 2004;Faurie et al 2005;Reberšek et al 2007). …”

Section: Parameters Of Electric Fieldmentioning

confidence: 99%

“…It has also been demonstrated by visualization of DNA interaction with the cell membrane that the electric field orientation plays an important role in gene electrotransfer (Golzio et al 2002;Faurie et al 2004;Faurie et al 2005;Reberšek et al 2007). Similar to small molecules, asymmetric DNA uptake is observed during electroporation (Mehrle et al 1985;Tekle et al 1991).…”

Section: Introduction Of Macromoleculesmentioning

confidence: 99%

See 1 more Smart Citation

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

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In this chapter, basics and mechanisms of electroporation are presented. Most important electric pulse parameters for electroporation efficiency for different applications that involve introduction of small molecules and macromolecules into the cell or cell membrane electrofusion are described. In all these applications, cell viability has to be preserved. However, in some biotechnological applications, such as liquid food sterilization or water treatment, electroporation is used as a method for efficient cell killing. For all the applications mentioned above, besides electric pulse parameters, other factors, such as electroporation medium composition and osmotic pressure, play significant roles in electroporation effectiveness. For controlled use of the method in all applications, the basic mechanisms of electroporation need to be known. The phenomenon was studied from the single-cell level and dense cell suspension that represents a simplified homogenous tissue model, to complex biological tissues. In the latter, different cell types and electric conductivity that change during the course of electric pulse application can significantly affect the effectiveness of the treatment. For such a complex situation, the design and use of suitable electrodes and theoretical modeling of electric field distribution within the tissue are essential. Electroporation as a universal method applicable to different cell types is used for different purposes. In medicine it is used for electrochemotherapy and genetherapy. In biotechnology it is used for water and liquid food sterilization and for transfection of bacteria, yeast, plant protoplast, and intact plant tissue. Understanding the phenomenon of electroporation, its mechanisms and optimization of all the parameters that affect electroporation is a prerequisite for successful treatment. In addition to the parameters mentioned above, different biological characteristics of treated cell affect the outcome of the treatment. Electroporation, gene electrotransfer and electrofusion are affected by cell membrane fluidity, cytoskeleton, and the presence of the cell wall in bacteria yeast and plant cells. Thus, electroporation parameters need to be specifically optimized for different cell types.

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Section: Parameters Of Electric Fieldmentioning

confidence: 99%

Section: Introduction Of Macromoleculesmentioning

confidence: 99%

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

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“…Another way of enlarging the permeabilized area without reducing the survival of the cells that has not been studied until lately is changing electric pulse direction [17]; i.e. delivering electrical pulses in different directions to the cells.…”

Section: Introductionmentioning

confidence: 99%

Optimization of bulk cell electrofusion in vitro for production of human–mouse heterohybridoma cells

Trontelj

Reberšek

Kandušer

et al. 2008

Bioelectrochemistry

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“…Further studies are needed to improve the transfection of the cells with a larger plasmid. The improvement of electroporators which can deliver different voltages during the transfection and the improvement of the electrodes could improve the transfection (Cepurniene et al, 2010;Rebersek et al, 2007). Furthermore, plasmid mobility is not well known.…”

Section: In Vitro Gene Transfer Progressesmentioning

confidence: 99%