Electroporation-mediated delivery of 3′-protected phosphodiester oligodeoxynucleotides to the skin

Regnier, Vincent; Tahiri, A; André, Nayara Delgado; Lemaître, M.; Doan, Trung Le; Préat, Véronique

doi:10.1016/s0168-3659(00)00223-6

Cited by 30 publications

(13 citation statements)

References 21 publications

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“…Topically applied oligonucleotides and DNA do not penetrate normal human SC, but removal of SC by tape stripping led to extensive penetration of oligonucleotides and DNA throughout the epidermis. Regnier et al [14] compared oligonucleotide penetration through intact and stripped hairless rat skin. Stripping increased the oligonucleotide concentration by 1 or 2 orders of magnitude (24-to 166-fold increase; table 2).…”

Section: Tape Stripping Versus Percutaneous Absorption and Penetrationmentioning

confidence: 99%

“…To increase permeability, chemical and physical approaches have been examined to decrease barrier properties. Physical approaches for skin penetration enhancement such as stripping [4][5][6][7][8][9][10][11][12], iontophoresis [13] and electroporation [13,14] have been evaluated. In addition, penetration enhancers and vesicle systems had been used to enhance permeability [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

et al. 2003

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The stratum corneum provides the first barrier to the percutaneous absorption of drugs as well as regulating water loss. This barrier limits the topical/transdermal delivery of drugs and biological macromolecules. Chemical and physical approaches have been examined to decrease these properties. Tape stripping is commonly used to disrupt the epidermal barrier, to enhance the delivery of drugs and to obtain information about stratum corneum function. Tape stripping results in the production and release of cytokines and co-stimulatory molecules and increases the humoral and cellular immune responses against peptide, protein and DNA antigens by a topical vaccination in vivo. This paper reviews the stripping method, experimental factors and its applications for penetration and topical vaccination.

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Section: Tape Stripping Versus Percutaneous Absorption and Penetrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

et al. 2003

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“…To increase permeability, chemical and physical approaches have been examined to lower SC barrier properties. These approaches include tape stripping [4][5][6][7] , iontophoresis [8][9][10] , electroporation [8,9,[11][12][13][14] and vesicular systems, such as liposomes and niosomes [15][16][17][18] .Among these approaches, liposomes (phospholipidbased artifi cial vesicles) and niosomes (non-ionic surfactant vesicles) are widely used to enhance drug permeation across the skin in cosmetic and dermatologic fi elds. In addition to conventional liposomes and niosomes, proliposomes and proniosomes are also used to enhance TT drug delivery.…”

mentioning

confidence: 99%

Liposomes and Niosomes as Topical Drug Delivery Systems

Choi

Maibach

2005

Skin Pharmacol Physiol

212

104

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The skin acts as a major target as well as a principle barrier for topical/transdermal (TT) drug delivery. The stratum corneum plays a crucial role in barrier function for TT drug delivery. Despite major research and development efforts in TT systems and the advantages of these routes, low stratum corneum permeability limits the usefulness of topical drug delivery. To overcome this, methods have been assessed to increase permeation. One controversial method is the use of vesicular systems, such as liposomes and niosomes, whose effectiveness depends on their physicochemical properties. This review focuses on the effect of liposomes and niosomes on enhancing drug penetration, and defines the effect of composition, size and type of the vesicular system on TT delivery.

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“…The mechanism of entry depends to some extent on ON chemistry; nonionic ONs such as methylphosphonates enter cells by passive diffusion in a concentration-dependent manner (Regnier et al, 2000), while ionic ONs such as unmodified phosphodiesters and phosphorothioates enter via receptor-mediated endocytosis (Yakubov et al, 1989). Zelphati and Szoka (1996) demonstrated that release of ONs from endosomes does not require acidification of the vesicles and that release takes place in the early stages of endocytosis, probably at the early endosome (EE) stage where vesicle pH ranges between 6.3 and 6.8 (Mellman, 1996).…”

Section: Cell Culture Studies With Onsmentioning

confidence: 99%

Cyclodextrins and Oligonucleotide Delivery to Solid Tumours

Dass

2004

Journal of Drug Targeting

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Cyclodextrins (CyDs) have traditionally been used for dissolving hydrophobic chemicals into aqueous media, and more recently, for inducing cholesterol efflux from lipid-laden cells as a proposed mechanism for reversal of cardiovascular disease. This review discusses the potential of delivering therapeutic oligonucleotides to solid tumours using CyD molecules. The physicochemical properties of these oligosaccharide molecules, and the barriers posed by the solid tumour itself, factors that affect may affect the uptake of oligonucleotides by CyDs, are the major foci of this review.

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Electroporation-mediated delivery of 3′-protected phosphodiester oligodeoxynucleotides to the skin

Cited by 30 publications

References 21 publications

Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

Liposomes and Niosomes as Topical Drug Delivery Systems

Cyclodextrins and Oligonucleotide Delivery to Solid Tumours

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