Electroporation in symmetric and asymmetric membranes

Genco, I.; Gliozzi, Alessandra; Relini, Annalisa; Robello, Mauro; Scalas, Enrico

doi:10.1016/0005-2736(93)90019-v

Cited by 85 publications

(60 citation statements)

References 32 publications

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“…[ 49,50,24] Furthermore, increasing line tensions are evidently related to decreasing diffusion coefficients, thus showing a clear connection between the intermolecular forces detected by AFM and the microviscosity in the lipid environment, as detected by two-focus SFCS.…”

Section: Force Measurements On L O and L D Domainsmentioning

confidence: 93%

Combined AFM and Two‐Focus SFCS Study of Raft‐Exhibiting Model Membranes

et al. 2006

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Dioleoylphosphatidylcholine/sphingomyelin/cholesterol (DOPC/SM/cholesterol) model membranes exhibit liquid-liquid phase separation and therefore provide a physical model for the putative liquid-ordered domains present in cells. Here we present a combination of atomic force microscopy (AFM) imaging, force measurements, confocal fluorescence imaging and two-focus scanning fluorescence correlation spectroscopy (two-focus SFCS) to obtain structural and dynamical information about this model membrane system. Partition coefficients and diffusion coefficients in the different phases were measured with two-focus SFCS for numerous fluorescent lipid analogues and proteins, while being directly related to the lateral organization of the membrane and its mechanical properties probed by AFM. Moreover we show how the combination of these different approaches is effective in reducing artifacts resulting from the use of a single technique.

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Section: Force Measurements On L O and L D Domainsmentioning

confidence: 93%

Combined AFM and Two‐Focus SFCS Study of Raft‐Exhibiting Model Membranes

et al. 2006

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“…Thus, ASA is likely to affect the delicate balance of forces presented between the polar heads of phospholipids, which include electrostatic repulsive and attractive interactions. It has been suggested that the stronger lateral repulsion between molecules of some lipids may contribute to pore formation [42]. This implies that, whereas cholesterol reduces the repulsive interactions between phospholipid polar heads, ASA may act opposite, facilitating the interactions between the positive charge of the PCh head and the negative charge of the adjacent PCh molecule, which finally results in easier formation of pores in the bilayer [43].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Effect of acetylsalicylic acid on the current–voltage characteristics of planar lipid membranes

Watała

Drapeza²,

Loban³

et al. 2009

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. contributed to the formation of metastable single pores, which facilitated ASA diffusion through a bilayer. Our data point out that ASA transport across the lipid bilayer takes place predominantly via the process of passive diffusion. In conclusion, the effects of ASA on lipid bilayer stability may contribute to drug transport through membrane lipid bilayers. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

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“…In particular, for the DPhPC membranes investigated here, the values of the first transition potential cluster around 400 mV [16].…”

Section: Introductionmentioning

confidence: 97%

“…It has been shown that the phenomenon is related to the lipid bilayer structure of the cell membrane. Thus, a quantitative study of electropermeabilization was made possible by experiments on model systems in well-controlled conditions, mainly bilayer lipid membranes (BLMs) [1,[8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Noise measurements in bilayer lipid membranes during electroporation

2000

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A study of voltage fluctuations in bilayer lipid membranes during electroporation and under current-clamp conditions is presented. Qualitative considerations based on the electroporation theory are used in order to explain the phenomenon on long time scale. Indeed, the current-clamp condition induces a feedback mechanism on the pore formation and therefore on the macroscopic conductance. Voltage fluctuations can thus be recorded. These fluctuations are nonstationary long-living and have a flicker power spectrum over nearly four decades of frequency between about 10 −2 and 10 2 Hz. The study of the fluctuations in the time domain has been performed by introducing an electrical model of the system formed by the membrane and the circuit under current-clamp configuration. The analysis of the time series gives a characteristic time of 100 ms for the circuitry response to the fragments of electroporation signals with characteristic times faster than 100 ms. During electroporation, the response to an external periodic stimulus in the frequency range 10 −1 -10 Hz shows that the system behaves linearly, even if voltage fluctuations are present.

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Electroporation in symmetric and asymmetric membranes

Cited by 85 publications

References 32 publications

Combined AFM and Two‐Focus SFCS Study of Raft‐Exhibiting Model Membranes

Combined AFM and Two‐Focus SFCS Study of Raft‐Exhibiting Model Membranes

Effect of acetylsalicylic acid on the current–voltage characteristics of planar lipid membranes

Noise measurements in bilayer lipid membranes during electroporation

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