Electroporation-based applications in biotechnology

Kotnik, Tadej; Frey, Wolfgang; Sack, Martin; Meglič, Saša Haberl; Peterka, Matjaž; Miklavčič, Damijan

doi:10.1016/j.tibtech.2015.06.002

Cited by 464 publications

(295 citation statements)

References 97 publications

(101 reference statements)

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“…The electric pulse can cause local destabilization of the cell membranes followed by electric field-induced transient pores, resulting in the direct delivery of extracellular objects into the cell. More details of fundamental physics of the electroporation can be found in many literatures (e.g., Al-Dosari and Gao, 2009;Gehl, 2003;Kotnik et al, 2015;Mehierhumbert and Guy, 2005). The electroporation treatment is able to be applied to cells adhered on a culture dish as well as cells suspended in a culture media.…”

Section: Physical Methods For Np Direct Deliverymentioning

confidence: 99%

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

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Nanoparticles have attracted much attention as a key material for new biomedical and pharmaceutical applications. For success in these applications, the nanoparticles are required to translocate across the cell membrane and to reach to inside of the cell. Among several translocation pathways of nanoparticles, the direct permeation pathway has a great advantage due to its high delivery efficacy. However, despite many research efforts, key properties and factors for driving the direct permeation of nanoparticle and its underlying mechanisms are far from being understood. In this article, experimental and computational studies regarding the direct permeation of nanoparticles across a cell membrane will be reviewed. Firstly, experimental studies on the nanoparticle-cell interactions, where spontaneous direct permeation of nanoparticles was observed, are reviewed. From the experimental studies, potential key physico-chemical properties of nanoparticles for their direct permeation are discussed. Secondly, physical methods such as electroporation and sonoporation for delivering nanoparticles into cells are reviewed. Current status of technologies for facilitating the direct permeation of nanoparticle is presented. Finally, we review molecular dynamics simulation studies and present the latest findings on the underlying molecular mechanisms of the direct permeation of nanoparticle.

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Section: Physical Methods For Np Direct Deliverymentioning

confidence: 99%

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

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“…These electric impulses have also demonstrated to promote eukaryotic cell fusion. 1,2 Electroporation is also an increasing recognized method for introducing DNA into bacterial cells and for promoting plasmid loss from bacteria. 3,4 In particular this technique was employed in transformation experiments to stimulate the uptake of plasmid DNA, and in direct transfer of genetic material to recipient cells not only among organisms of the same species, but also among bacteria genetically not correlated to each other.…”

Section: Methodsmentioning

confidence: 99%

“…3,4 In particular this technique was employed in transformation experiments to stimulate the uptake of plasmid DNA, and in direct transfer of genetic material to recipient cells not only among organisms of the same species, but also among bacteria genetically not correlated to each other. 2 Many different experimental conditions were also explored to enhance the efficiency of DNA and proteins exchange. [5][6][7][8] Previous experiments from this laboratory showed that an apparatus, used for endodontic treatments, that generated a high frequency alternating current (Endox® Endodontic System (Endox) 9 presented many analogies with the electric pulses generator employed for electroporation.…”

Section: Methodsmentioning

confidence: 99%

“…The strong reduction of the number of recombinants when DNAse was added suggests that the free genetic material is digested by the enzyme, The low number of recombinants still obtained under these experimental conditions might be due to the presence of residual DNA not completely eliminated by the enzyme. On the other hand, the pores appear stable and can be seen under an electronic microscope, [2][3][4][5][6][7][8][9][10][11][12][13] bacterial cells that are not totally killed by the treatment and required about 1 hour to resume their original growth rate, 11 might have enough time to acquire the residual genetic material or establish a direct contact between each other. It is also possible that when pores are formed might fit together with those of the partner cells establishing a direct cell to cell connection enabling all cytoplasmic material to be exchange.…”

Section: Methodsmentioning

confidence: 99%

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Direct transfer of genetic material between two Escherichia coli K12 strains by electroporation

Marchese

Debbia²

2017

Microbiol Res (Pavia)

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A direct transfer of plasmid pBP517 from C600 to J53 Escherichia coli K12 strains by electroporation, either by the standard method, or in the presence of 20 µg/mL of DNAse was carried out. In a standard experiment, donor and recipient bacteria were mixed and subjected to the electroporation procedure, about 2700 (range 1600-3700) recombinants were found, while no colonies were detected from non treated bacteria. When the same tests were performed at the presence of DNAse the number of recombinants fell to about 200 (range 183-218). This difference between the number of colonies found in the presence or in absence of DNAse was observed every time the tests were repeated. According to these observations, plasmid DNA has been transferred from donor to recipient cells via electroporation also when DNAse was added. Since the free genetic material is destroyed by the enzyme and recombination takes place it has been hypothesized that there must be a direct contact between the partner cells.

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“…EP treatment has serious effects on the structure of biological tissues [7]. When a high-intensity, external electric field is applied, a critical electric potential is induced across the cell membrane, which leads to rapid electrical breakdown and local structural changes of the cell membrane and the cell wall [8].…”

Section: Introductionmentioning

confidence: 99%

Application of Electroporation Technique in Biofuel Processing

et al. 2017

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Abstract. Biofuels production is mostly oriented with fermentation process, which requires fermentable sugar as nutrient for microbial growth. Lignocellulosic biomass (LCB) represents the most attractive, low-cost feedstock for biofuel production, it is now arousing great interest. The cellulose that is embedded in the lignin matrix has an insoluble, highlycrystalline structure, so it is difficult to hydrolyze into fermentable sugar or cell protein. On the other hand, microbial lipid has been studying as substitute of plant oils or animal fat to produce biodiesel. It is still a great challenge to extract maximum lipid from microbial cells (yeast, fungi, algae) investing minimum energy.Electroporation (EP) of LCB results a significant increase in cell conductivity and permeability caused due to the application of an external electric field. EP is required to alter the size and structure of the biomass, to reduce the cellulose crystallinity, and increase their porosity as well as chemical composition, so that the hydrolysis of the carbohydrate fraction to monomeric sugars can be achieved rapidly and with greater yields. Furthermore, EP has a great potential to disrupt the microbial cell walls within few seconds to bring out the intracellular materials (lipid) to the solution. Therefore, this study aims to describe the challenges and prospect of application of EP technique in biofuels processing.

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Electroporation-based applications in biotechnology

Cited by 464 publications

References 97 publications

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Direct transfer of genetic material between two Escherichia coli K12 strains by electroporation

Application of Electroporation Technique in Biofuel Processing

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