Electropolymerisable pyrrole-oligonucleotide: synthesis and analysis of ODN hybridisation by fluorescence and QCM

Lassalle, N.; Roget, André; Livache, Thierry; Mailley, Pascal; Vieil, E.

doi:10.1016/s0039-9140(01)00491-x

Cited by 34 publications

(15 citation statements)

References 13 publications

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“…They were achieved by introducing an intercalator or groove binder, and by monitoring its redox reaction on a transducer whose surface is modified with capture probe (CP) DNAs [17][18][19][20]. On the other hand, redox-active polymer films [21,22] or a molecular aggregate [23] have been examined for immobilizing CP-DNAs. Since the reaction scheme for the present DNA sensors is rather complicated, these types of DNA electrode having particular EC activity would be useful for providing even more simplified methods.…”

Section: Responses For Dna Attachment and Hybridizationmentioning

confidence: 99%

Poly(hydroquinone)-coated electrode for immobilizing of 5′-amine functioned capture probe DNA and electrochemical response to DNA hybridization

Nakano¹,

Hirayama²,

Toguchi³

et al. 2006

Science and Technology of Advanced Materials

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Enzymatically polymerized hydroquinone, PHQ, was applied for polymer-coated electrode whose surface was further modified with 5 0 -amine dodecamer DNAs (capture probe DNA, CP-DNA) by taking advantage of Michael reaction. The film-forming property of PHQ on graphite substrate surfaces was confirmed to be satisfactory by AFM imaging. Cyclic voltammetric (CV) measurements showed that the PHQ-modified graphite electrode gave well-defined redox waves showing characteristics for surface process. The pH dependence of the formal potential, E 1/2 , suggested that the electrode reaction occurred by two-proton and two-electron mechanism (À59 mV per a pH decade). CVs also gave the specific amount of PHQ adsorption of 0.52 or 0.83 nmol cm À2 (monomer unit) for the different electrode preparations. This was indicative of two-or three-monolayer adsorption of PHQ. For applications of gene detection, the immobilization reaction including the CP-DNA hybridization was studied by microgravimetric analysis using a quartz-crystal microbalance (QCM). Summaries for the two different runs were 1.01 and 0.69 nmol cm À2 (monomer unit) for PHQ adsorption on gold surfaces, 0.19 and 0.15 nmol cm À2 for CP-DNA attachment on the PHQ/Au, and 0.14 and 0.11 nmol cm À2 for hybridization with the complementary DNA on the CP-DNA/PHQ/Au, respectively. Importantly, monitoring of the series of the experiments were possible by measuring the voltammetric properties of the electrode; the distinct redox waves due to PHQ-electrode reaction are suppressed upon immobilizing of the CP-DNA, and its hybridization further suppressed the redox activity. Neither treating of the PHQ-electrode with native DNAs nor treating of CP-DNA/PHQ-electrode with non-target DNA gave no noticeable responses. A possible mechanism for the electrode response was discussed briefly based on electrochemical QCM measurements. We think these observations are important as the basis of DNA hybridization sensor that enables totally, label-free electrochemical detection of the target DNA. r

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Section: Responses For Dna Attachment and Hybridizationmentioning

confidence: 99%

Poly(hydroquinone)-coated electrode for immobilizing of 5′-amine functioned capture probe DNA and electrochemical response to DNA hybridization

Nakano¹,

Hirayama²,

Toguchi³

et al. 2006

Science and Technology of Advanced Materials

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“…Livache et al developed a process that utilizes pyrrole monomer bearing an ODN to copolymerize with pyrrole ( Fig. 3a) [54,55] allowing the immobilization of multiple probes on electrode arrays. Later the same group used a similar process to prepare an ODN array consisting of a matrix of 48 addressable 50-mm microelectrodes that was applied to detect hepatitis C virus in blood samples [56].…”

Section: Immobilization Of Dna Probesmentioning

confidence: 99%

“…Livache et al developed a pyrrole monomer bearing an ODN (Fig. 8, monomer 1) and copolymerized this with pyrrole to realize addressable multiple probe immobilization [54,55]. The hybridization was detected by photocurrent spectroscopy [92].…”

Section: Dna Sensors Based On Polypyrrole and Its Derivativesmentioning

confidence: 99%

Conducting polymers for electrochemical DNA sensing

et al. 2009

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“…The immobilization of ODNs functionalized by one pyrrole group has been successfully carried out by electropolymerization or copolymerization with regular pyrrole [23,30,105,106]. Indeed, an electrochemical copolymer- ization process with unsubstituted monomers was often required for biopolymer formation.…”

Section: Electropolymerization Of Bioreceptorsmentioning

confidence: 99%

Affinity Biosensors Based on Electropolymerized Films

Cosnier

2005

Electroanalysis

145

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This review gives an overview on different types of affinity biosensors based on electropolymerized polymer films that are becoming an important class of analytical tools. These affinity biosensors may be classified according to the strategy used for their fabrication, namely entrapment within polymers during their electrochemical growth, simple adsorption onto electropolymerized films, chemical coupling or affinity interactions between bioreceptors and electropolymerized films or direct electrochemical polymerization of the bioreceptor itself. Recently opened perspectives and potential research directions are also discussed.

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Electropolymerisable pyrrole-oligonucleotide: synthesis and analysis of ODN hybridisation by fluorescence and QCM

Cited by 34 publications

References 13 publications

Poly(hydroquinone)-coated electrode for immobilizing of 5′-amine functioned capture probe DNA and electrochemical response to DNA hybridization

Poly(hydroquinone)-coated electrode for immobilizing of 5′-amine functioned capture probe DNA and electrochemical response to DNA hybridization

Conducting polymers for electrochemical DNA sensing

Affinity Biosensors Based on Electropolymerized Films

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