Electrophysiology of Epithelial Sodium Channel (ENaC) Embedded in Supported Lipid Bilayer Using a Single Nanopore Chip

Khan, Muhammad S.; Dosoky, Noura S.; Mustafa, Ghulam; Patel, Darayas; Berdiev, Bakhrom K.; Williams, John D.

doi:10.1021/acs.langmuir.7b02404

Cited by 23 publications

(20 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, force–distance curves indicated that the POPC/Chol bilayers were slightly thicker upon the increasing of cholesterol content, ranging from 4.0 to 5.5 nm (increasing approximately 1.0 nm) (Figure 1). These values are comparable to the thickness of supported lipid bilayers previously determined by AFM [29,30]. As previous studies state, the interplay between cholesterol and phospholipid molecules promotes lateral ordering of lipid acyl chain leading to a lipid phase change from l d phase to l o phase [31,32,33].…”

Section: Discussionsupporting

confidence: 85%

Cholesterol Increases Lipid Binding Rate and Changes Binding Behavior of Bacillus thuringiensis Cytolytic Protein

Tharad

Üzülmez

Promdonkoy

et al. 2018

IJMS

View full text Add to dashboard Cite

Cytolytic protein (Cyt) is a member of insecticidal proteins produced by Bacillus thuringiensis. Cyt protein has activity against insect cells and mammalian cells, which differ in lipid and cholesterol composition. This study presents the lipid binding behavior of Cyt2Aa2 protein on model membranes containing different levels of cholesterol content by combining Quartz Crystal Microbalance with Dissipation (QCM-D) and Atomic Force Microscopy (AFM). QCM-D results revealed that cholesterol enhances the binding rate of Cyt2Aa2 protein onto lipid bilayers. In addition, the thicker lipid bilayer was observed for the highest cholesterol content. These results were confirmed by AFM. The analysis of protein surface coverage as a function of time showed a slower process for 5:0 and 5:0.2 (POPC:Chol) ratios than for 5:1 and 5:2 (POPC:Chol) ratios. Significantly, the Cyt2Aa2-lipid binding behavior and the protein–lipid layer were different for the 5:3 (POPC:Chol) ratio. Furthermore, AFM images revealed a transformation of Cyt2Aa2/lipid layer structure from strip pattern to ring shape structures (which showed a strong repulsion with AFM tip). In summary, cholesterol increases the binding rate and alters the lipid binding behavior of Cyt2Aa2 protein, although it is not required for Cyt2Aa2 protein binding onto lipid bilayers.

show abstract

Section: Discussionsupporting

confidence: 85%

Cholesterol Increases Lipid Binding Rate and Changes Binding Behavior of Bacillus thuringiensis Cytolytic Protein

Tharad

Üzülmez

Promdonkoy

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…The permeabiltization of cell membrane would increase when the external field was applied, which would lead to bacterial lysis and death. In our C 4 D system, the measured current through the media in the capillary was less than 1 μA at the excitation of 25 Vpp, though the voltage was larger than the one used in electric double layer [52,53,54]. The maximum conductivity of the media was 0.286 mS/cm measured by METTLER TOLEDOLE 740 (METTLER TOLEDO, Shanghai, China), that is, the resistivity is 416.7 Ω·cm.…”

Section: Resultsmentioning

confidence: 92%

Bacterial Concentration Detection using a PCB-based Contactless Conductivity Sensor

Zhang

Zang

et al. 2019

Micromachines

View full text Add to dashboard Cite

Capacitively coupled contactless conductivity detection (C4D) is an improved approach to avoid the problems of labor-intensive, time-consuming and insufficient accuracy of plate count as well as the high-cost apparatus of flow cytometry (FCM) in bacterial counting. This article describes a novel electrode-integrated printed-circuit-board (PCB)-based C4D device, which supports the simple and safe exchange of capillaries and improves the sensitivity and repeatability of the contactless detection. Furthermore, no syringe pump is needed in the detection, it reduces the system size, and, more importantly, avoids the effect on the bacteria due to high pressure. The recovered bacteria after C4D detection at excitation of 25 Vpp and 60–120 kHz were analyzed by flow cytometry, and a survival rate higher than 96% was given. It was verified that C4D detection did not influence the bacterial viability. Moreover, bacteria concentrations from 106 cells/mL to 108 cells/mL were measured in a linear range, and relative standard deviation (RSD) is below 0.2%. In addition, the effects on bacteria and C4D from background solutions were discussed. In contrast to common methods used in most laboratories, this method may provide a simple solution to in situ detection of bacterial cultures.

show abstract

“…Moreover, they used AFM to characterize the presence and thickness of supported lipid membrane (SLM) on the nanopore chip in another functionality measurement of SLM. It follows that AFM can be a useful tool for functionality characterization of biological membranes [ 76 ].…”

Section: Friction Measurement By Afmmentioning

confidence: 99%

Friction Determination by Atomic Force Microscopy in Field of Biochemical Science

Wang

2018

Micromachines

View full text Add to dashboard Cite

Atomic force microscopy (AFM) is an analytical nanotechnology in friction determination between microscale and nanoscale surfaces. AFM has advantages in mechanical measurement, including high sensitivity, resolution, accuracy, and simplicity of operation. This paper will introduce the principles of mechanical measurement by using AFM and reviewing the progress of AFM methods in determining frictions in the field of biochemical science over the past decade. While three friction measurement assays—friction morphology, friction curve and friction process in experimental cases—are mainly introduced, important advances of technology, facilitating future development of AFM are also discussed. In addition to the principles and advances, the authors also give an overview of the shortcomings and restrictions of current AFM methods, and propose potential directions of AFM techniques by combining it with other well-established characterization techniques. AFM methods are expected to see an increase in development and attract wide attention in scientific research.

show abstract

Electrophysiology of Epithelial Sodium Channel (ENaC) Embedded in Supported Lipid Bilayer Using a Single Nanopore Chip

Cited by 23 publications

References 84 publications

Cholesterol Increases Lipid Binding Rate and Changes Binding Behavior of Bacillus thuringiensis Cytolytic Protein

Cholesterol Increases Lipid Binding Rate and Changes Binding Behavior of Bacillus thuringiensis Cytolytic Protein

Bacterial Concentration Detection using a PCB-based Contactless Conductivity Sensor

Friction Determination by Atomic Force Microscopy in Field of Biochemical Science

Contact Info

Product

Resources

About