Electrophysiological Characterization of Tight Junctional Pathway of Rabbit Cornea Treated with Ophthalmic Ingredients

Nakamura, Tadahiro; Yamada, Mikiko; Teshima, Mugen; Nakashima, Makoto; To, Hideto; Ichikawa, Nobuhiro; Sasaki, Hidetada

doi:10.1248/bpb.30.2360

Cited by 51 publications

(37 citation statements)

References 23 publications

(28 reference statements)

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“…12,16) We found a significant correlation between the cytotoxicity of preservatives and paracellular permeability of FD-4, and between the cytotoxicity of preservatives and conductance (reciprocal value of TEER). 4) Rojanasakul et al 17) compared TEER of various epithelial tissues and demonstrated that the corneal membrane is very tight compared with other tissues. During preincubation, TEER reached approximately 1000 ohmϫcm 2 within 80 min after reaching the cornea, and the permeability of FD-4 was extremely low.…”

Section: Discussionmentioning

confidence: 99%

“…4) Transepithelial electrical resistance (TEER) was the most sensitive electrophysiological parameter for changes occurring with the exposure of eye drops to the corneal epithelium. We found two significant correlations between the cytotoxicity of preservatives and corneal paracellular permeability of fluorescein isothiocyanate dextran (FD-4, average molecular weight of 4400) and between the cytotoxicity of preservatives and conductance (reciprocal value of TEER).…”

mentioning

confidence: 99%

“…We found two significant correlations between the cytotoxicity of preservatives and corneal paracellular permeability of fluorescein isothiocyanate dextran (FD-4, average molecular weight of 4400) and between the cytotoxicity of preservatives and conductance (reciprocal value of TEER). 4) Benzalkonium chloride (BAC) is a preservative often used for eye drops. Animal studies, in vitro studies and in vivo experiments have demonstrated various adverse effects of BAC.…”

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confidence: 99%

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Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow

Nakamura

Teshima

Kitahara

et al. 2010

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow

Nakamura

Teshima

Kitahara

et al. 2010

Biological & Pharmaceutical Bulletin

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“…On the other hand, numerous in vitro, ex vivo, and in vivo studies have revealed the deleterious corneal effects of BAC that include destabilization of the tear film, 1 death of corneal epithelium cells, 2,3 disruption of superficial cell membranes, loss of microvilli [4][5][6][7] and the reduction of the corneal epithelial barrier function. 8 The side effects of BAC depend both on its concentration and the period of exposure. Chronic topical administration of BAC in rabbit eyes induces dry eye syndrome with damage to the cornea and the conjunctiva.…”

Section: Introductionmentioning

confidence: 99%

Polyoxyethylene Hydrogenated Castor Oil Modulates Benzalkonium Chloride Toxicity: Comparison of Acute Corneal Barrier Dysfunction Induced by Travoprost Z and Travoprost

Uematsu

Kumagami

Shimoda

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

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Methods: Corneal transepithelial electrical resistance (TER) was measured in live white Japanese rabbits by two Ag/AgCl electrodes placed in the anterior aqueous chamber and on the cornea. We evaluated corneal TER changes after a 60-s exposure to travoprost Z, travoprost, and 0.015% BAC.Similarly, TER changes were evaluated after corneas were exposed for 60 s to the travoprost additives EDTA, boric acid, mannitol, trometamol, and polyoxyethylene hydrogenated castor oil 40 (HCO-40) with or without BAC. Corneal damage was examined after exposure to BAC with or without travoprost additives using scanning electron microscopy (SEM) and a cytotoxicity assay.Results: Although no decreases of TER were noted after exposure to travoprost Z with sofZia and travoprost with 0.015% BAC, a significant decrease of corneal TER was observed after 0.015% BAC exposure. With the exception of for BAC, no corneal TER decreases were observed for any travoprost additives. After corneal exposure to travoprost additives with BAC, HCO-40 was able to prevent the BAC-induced TER decrease. SEM observations and the cytotoxicity assay confirmed that there was a remarkable improvement of BAC-induced corneal epithelial toxicity after addition of HCO-40 to the BAC.

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“…Compared with the original formulation (0.005%), the new formulation has a 4-fold increase in the amount of benzalkonium chloride (BAK) (0.020%). 22 Therefore, the strategy was to reduce the concentration of the drug and increase the concentration of BAK, a quaternary ammonium salt with a high power germicidal and disinfectant, that can increase the corneal penetration 23 and intraocular bioavailability of topically applied medications. This characteristic can be explained by a loss of tight junctions in the corneal epithelium, favoring corneal penetration.…”

Section: Introductionmentioning

confidence: 99%

Bimatoprost 0.01% vs bimatoprost 0.03%: a 12-month prospective trial of clinical and in vivo confocal microscopy in glaucoma patients

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Nardi

Piaggi

et al. 2014

Eye

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Results Global clinical score (the sum of pruritus, stinging/burning, blurred vision, sticky eye sensation, eye dryness sensation, and foreign body sensation) significantly decreased in the bimatoprost 0.01% group from baseline 4.7±3.8 to 2.9±2.3 (Po0.001) and 2.5±2.0 (Po0.001) at 6-month and 12-month follow-ups, respectively. Comparison between groups showed differences at both follow-up visits (P ¼ 0.003 and Po0.001, respectively). In vivo confocal microscopy revealed a significant increase in goblet cell density in the bimatoprost 0.01% group compared with the bimatoprost 0.03% group (Po0.001 at both follow-up visits). All functional parameters and conjunctival hyperemia improved in the bimatoprost 0.01% group at each follow-up visit (Po0.05) and in comparison with bimatoprost 0.03% (Po0.05). ConclusionThe results of this trial suggest that bimatoprost 0.01% eye drops seem to decrease the ocular discomfort with respect to bimatoprost 0.03% eye drops.

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Electrophysiological Characterization of Tight Junctional Pathway of Rabbit Cornea Treated with Ophthalmic Ingredients

Cited by 51 publications

References 23 publications

Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow

Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow

Polyoxyethylene Hydrogenated Castor Oil Modulates Benzalkonium Chloride Toxicity: Comparison of Acute Corneal Barrier Dysfunction Induced by Travoprost Z and Travoprost

Bimatoprost 0.01% vs bimatoprost 0.03%: a 12-month prospective trial of clinical and in vivo confocal microscopy in glaucoma patients

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