Electrophysiological characterization of functionally distinct 5-hydroxytryptamine receptors on guinea-pig submucous plexus

Surprenant, Annmarie; Crist, J.

doi:10.1016/0306-4522(88)90331-4

Cited by 111 publications

(59 citation statements)

References 31 publications

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“…It should be noted that on guinea-pig submucous plexus neurones (Surprenant & Crist, 1988) Recent electrophysiological experiments have shown that different types of channel can be activated by 5-HT3 receptors. Thus, the channels activated by 5-HT on guinea-pig submucous plexus neurones have conductances of 9 and 15pS (Derkach et al, 1989), whereas the 5-HT3-induced channel conductance on N1E-1 15 cells is 0.3 pS (Lambert et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…(Newberry & Gilbert, 1989), it was interesting to note that (+ )-tubocurarine antagonized the 5-HT3 response of NG 108-15 and NIE-115 cells (Yakel & Jackson, 1988;Peters et al, 1990) in nanomolar concentrations, but 10-500puM was needed to antagonize the fast depolarization of guinea-pig submucous plexus neurones (Surprenant & Crist, 1988) and guinea-pig coeliac ganglion cells (Wallis & Dun, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Evidence that the 5‐HT₃ receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Newberry

Cheshire

Gilbert

1991

British J Pharmacology

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1 Using grease-gap recordings from the isolated superior cervical ganglion of mouse, rat and guinea-pig, we have compared the depolarization evoked by 5-hydroxytryptamine (5-HT) with that evoked by the selective 5-HT3 receptor agonist 2-methyl-5-HT (2-Me-5-HT). 2 The maximum depolarization induced by 2-Me-5-HT was smaller than that induced by 5-HT in all three species, and particularly in the guinea-pig. 3 The 5-HT2 receptor antagonist ketanserin (1 UM) caused a clear rightward shift of the dose-response curve to 5-HT on the guinea-pig ganglion, but not on the mouse or rat ganglion. Spiperone (0.03pM) had a quantitatively similar action to ketanserin (O.1pM) on the 5-HT dose-response curve of the guinea-pig ganglion. Ketanserin had no significant effect on the dose-response curve to 2-Me-5-HT on any of these ganglia. 4 Using 2-Me-5-HT as the agonist, we determined the pA2 values for two 5-HT3 receptor antagonists. The potency of ICS 205-930 varied by approximately 100 fold between the species and that of (+)-tubocurarine varied by over 1000 fold. The differences in the pA2 values of these compounds varied independently among the species. 5 We conclude that 5-HT3 receptors are present on the superior cervical ganglion from the rat, mouse and guinea-pig, but these receptors may be pharmacologically distinct from each other. In addition, the depolarization of the guinea-pig superior cervical ganglion by low concentrations of 5-HT is largely mediated by ketanserin-sensitive receptors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence that the 5‐HT₃ receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Newberry

Cheshire

Gilbert

1991

British J Pharmacology

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“…Surprenant & Crist, 1988;Mawe et al, 1989;Schemann, 1991). Hyperpolarization of nerve cells is mediated via 5-HTIA receptors (Galligan et al, 1988) and activation of 5-HT4 receptors on nerve endings enhances transmitter release (Taniyama et al, 1991;Kilbinger & Wolf, 1992).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the role of 5‐HT₃ and 5‐HT₄ receptors in ascending and descending reflexes to the circular muscle of guinea‐pig small intestine

Shi¹,

Bornstein²,

Furness³

1994

British J Pharmacology

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1 The present study was undertaken to ascertain whether 5-hydroxytryptamine (5-HT) acting at either 5-HT3 or 5-HT4 receptors plays a significant role in motility reflexes in the guinea-pig small intestine. 2 An isolated segment of small intestine was opened along its mesenteric border and pinned, mucosa uppermost, in a three chambered organ bath so that the oral, middle and anal regions of a single preparation could be separately superfused. 3 Conventional intracellular recording methods were used to monitor the responses of the circular muscle in the oral or the anal end chambers when distension was applied in either of the other two chambers or the mucosal villi were compressed in the middle chamber. Drugs were added to the middle chamber.4 5-HT3 receptor antagonists (tropisetron, 0.1-101iM; granisetron, 1 11M and BRL46470, 1 gM) depressed the ascending excitatory reflex evoked by these stimuli but had no effect on the descending inhibitory reflex. The depression of the excitatory reflex was observed whether the reflex was evoked from the chamber containing the drug or was simply conducted, via interneurones, through this chamber. 5The 5-HT4 receptor antagonist, (1 PM), had no significant effect on either the ascending or descending reflex pathways. However, 5-HT4 receptors were present as cisapride (0.1 M) significantly enhanced the ascending excitation without affecting the descending inhibition. This effect of cisapride was converted to a significant depression of the ascending reflex by SDZ 205-557. 6 The results suggest that 5-HT3, but not 5-HT4, receptors play an important role in the ascending excitatory reflex and that these receptors may be on interneurones in the reflex pathway.

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“…Local application of 5-HT has shown that the ionotropic receptors for serotonin, 5-HT 3 receptors, are found on a large proportion of both myenteric and submucosal neurons [76,77,83,118,130,142]. However, neurons exhibiting EPSPs mediated by 5-HT 3 receptors are rare (< 10% of those studied) [142].…”

Section: Fast Epsps and The Receptors That Mediate Themmentioning

confidence: 99%

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

Gwynne

Bornstein²

2007

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Digestion and absorption of nutrients and the secretion and reabsorption of fluid in the gastrointestinal tract are regulated by neurons of the enteric nervous system (ENS), the extensive peripheral nerve network contained within the intestinal wall. The ENS is an important physiological model for the study of neural networks since it is both complex and accessible. At least 20 different neurochemically and functionally distinct classes of enteric neurons have been identified in the guinea pig ileum. These neurons express a wide range of ionotropic and metabotropic receptors. Synaptic potentials mediated by ionotropic receptors such as the nicotinic acetylcholine receptor, P2X purinoceptors and 5-HT 3 receptors are seen in many enteric neurons. However, prominent synaptic potentials mediated by metabotropic receptors, like the P2Y 1 receptor and the NK 1 receptor, are also seen in these neurons. Studies of synaptic transmission between the different neuron classes within the enteric neural pathways have shown that both ionotropic and metabotropic synaptic potentials play major roles at distinct synapses within simple reflex pathways. However, there are still functional synapses at which no known transmitter or receptor has been identified. This review describes the identified roles for both ionotropic and metabotropic neurotransmission at functionally defined synapses within the guinea pig ileum ENS. It is concluded that metabotropic synaptic potentials act as primary transmitters at some synapses. It is suggested identification of the interactions between different synaptic potentials in the production of complex behaviours will require the use of well validated computer models of the enteric neural circuitry.

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Electrophysiological characterization of functionally distinct 5-hydroxytryptamine receptors on guinea-pig submucous plexus

Cited by 111 publications

References 31 publications

Evidence that the 5‐HT₃ receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Evidence that the 5‐HT₃ receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Investigation of the role of 5‐HT₃ and 5‐HT₄ receptors in ascending and descending reflexes to the circular muscle of guinea‐pig small intestine

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

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Electrophysiological characterization of functionally distinct 5-hydroxytryptamine receptors on guinea-pig submucous plexus

Cited by 111 publications

References 31 publications

Evidence that the 5‐HT3 receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Evidence that the 5‐HT3 receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Investigation of the role of 5‐HT3 and 5‐HT4 receptors in ascending and descending reflexes to the circular muscle of guinea‐pig small intestine

Synaptic Transmission at Functionally Identified Synapses in the Enteric Nervous System: Roles for Both Ionotropic and Metabotropic Receptors

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Evidence that the 5‐HT₃ receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Evidence that the 5‐HT₃ receptors of the rat, mouse and guinea‐pig superior cervical ganglion may be different

Investigation of the role of 5‐HT₃ and 5‐HT₄ receptors in ascending and descending reflexes to the circular muscle of guinea‐pig small intestine