Electrophysiological and morphological properties of Cajal–Retzius cells with different ontogenetic origins

Sava, Bogdan Aurel; Dávid, Csaba; Teissier, Anne; Pierani, Alessandra; Staiger, Jochen F.; Luhmann, Heiko J.; Kilb, Werner

doi:10.1016/j.neuroscience.2010.02.043

Cited by 31 publications

(28 citation statements)

References 57 publications

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“…Cajal-Retzius neurons showed reliable subthreshold resonance in all investigated cells, which corresponds to the homogenous membrane properties observed for different Cajal-Retzius cell subtypes (Sava et al, 2010). The resonance frequencies of GABAergic interneurons in this study are considerably different from the rather high resonance frequencies of up to 50 Hz observed in hippocampal fast spiking basket cells (Pike et al, 2000), but comparable to other types of interneurons with subthreshold resonance in the delta to theta frequency range (Solinas et al, 2007;Zemankovics et al, 2010;Boehlen et al, 2011;van Brederode and Berger, 2011).…”

Section: Resonance Properties Of Different Neuronal Populationssupporting

confidence: 75%

“…Tight-seal whole-cell recordings induce an additional leak conductance via the seal between the membrane and the pipette, which may influence the resting membrane potential (Sava et al, 2010). In our recordings this error accounts for a slight depolarization, thus under "undisturbed" conditions the resting membrane potentials most probably is between -63 and -82 mV ( Table 1).…”

Section: Leak Conductance May Influence the Resonance Properties Of Nmentioning

confidence: 85%

“…Materials and Methods 29 cells in tangential slices (Achilles et al, 2007) and from cortical plate, layer II/III and layer V pyramidal neurons as well as subplate cells in coronal slices Hanganu et al, 2001;Hirsch and Luhmann, 2008) and GABAergic interneurons from GAD67-GFP knock in mice, according to protocols published before (Sava et al, 2010). Using an upright microscope ( to the formula delivered by (Tyzio et al, 2003), to correct voltage errors caused by the short circuit via the seal conductance.…”

Section: Electrophysiological Recordingsmentioning

confidence: 99%

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Resonance properties of different neuronal populations in the immature mouse neocortex

Sun

Luhmann

Kilb

2012

Eur J of Neuroscience

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In vivo recordings in the immature neocortex revealed spontaneous and sensory‐driven oscillatory activity from delta (0.5–4 Hz) to gamma (30–100 Hz) frequencies. In order to investigate whether the resonance properties of distinct neuronal populations in the immature neocortex contribute to these network oscillations, we performed whole‐cell patch‐clamp recordings from visually identified neurons in tangential and coronal neocortical slices from postnatal day (P)0–P7 C57Bl/6 mice. Subthreshold resonance was analysed by sinusoidal current injection of varying frequency. All Cajal–Retzius cells showed subthreshold resonance, with an average frequency of 2.6 ± 0.1 Hz (n = 60), which was massively reduced by ZD7288, a blocker of hyperpolarization‐activated cation currents. Approximately 65.6% (n = 61) of the supragranular pyramidal neurons showed subthreshold resonance, with an average frequency of 1.4 ± 0.1 Hz (n = 40). Application of Ni2+ suppressed subthreshold resonance, suggesting that low‐threshold calcium currents contribute to resonance in these neurons. Approximately 63.6% (n = 77) of the layer V pyramidal neurons showed subthreshold resonance, with an average frequency of 1.4 ± 0.2 Hz (n = 49), which was abolished by ZD7288. Only 44.1% (n = 59) of the subplate neurons showed subthreshold resonance, with an average frequency of 1.3 ± 0.2 Hz (n = 26) and a small resonance strength. In summary, these results demonstrate that neurons in all investigated layers show resonance behavior, with either hyperpolarization‐activated cation or low‐threshold calcium currents contributing to the subthreshold resonance. The observed resonance frequencies are in the range of slow activity patterns observed in the immature neocortex, suggesting that subthreshold resonance may support the generation of this activity.

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Section: Resonance Properties Of Different Neuronal Populationssupporting

confidence: 75%

Section: Leak Conductance May Influence the Resonance Properties Of Nmentioning

confidence: 85%

Section: Electrophysiological Recordingsmentioning

confidence: 99%

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Resonance properties of different neuronal populations in the immature mouse neocortex

Sun

Luhmann

Kilb

2012

Eur J of Neuroscience

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“…In order to investigate whether synaptic GABAergic activity is capable to significantly influence the [Cl − ] i and the GABAergic rheobase shift, we used the cholinergic agonist carbachol to increase the frequency of GABAergic postsynaptic potentials [32]. Control experiments under whole-cell and voltage-clamp conditions revealed that bath application of carbachol-induced barrages (30-100 μM) of postsynaptic currents that in CRc were completely blocked by 10 μM gabazine (n=10 cells), indicating that carbacholinduced postsynaptic events were exclusively mediated by GABA A receptors.…”

Section: Carbachol-induced Gabaergic Inputs Shift Gabaergic Excitationmentioning

confidence: 99%

Activity-dependent scaling of GABAergic excitation by dynamic Cl− changes in Cajal–Retzius cells

Kolbaev

Luhmann

Kilb

2011

Pflugers Arch - Eur J Physiol

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To unravel the functional implications of activity-dependent Cl- changes during early stages of neuronal development, we determined which changes in the GABA reversal potential (E (GABA)) and GABAergic rheobase shifts were induced by episodes of GABA(A) receptor activation using gramicidin-perforated patch-clamp recordings from Cajal-Retzius cells in tangential cortical slices of newborn mice. Under this condition, focal application of the GABA(A) agonist muscimol (10 μM) depolarized the membrane by 15 ± 0.8 mV (n = 35). Such subthreshold GABAergic depolarizations considerably reduced the rheobase, corresponding to an excitatory action. After repetitive focal muscimol applications (50 pulses at 0.5 Hz) a significant reduction of E (GABA) and an attenuation of the excitatory GABAergic rheobase shift were observed, while the GABAergic membrane conductance and the absolute value of the rheobase were unaltered after the muscimol pulses. Bath application of 100 μM carbachol induced bursts of spontaneous GABAergic postsynaptic potentials. Both, E (GABA) and the excitatory GABAergic rheobase shift was significantly reduced after such barrage of carbachol-induced GABAergic postsynaptic potentials, while neither the GABAergic membrane conductance nor the absolute value of the rheobase was affected under this condition. Both results indicate that GABAergic activity itself can limit the excitatory effects of GABA(A) receptor activation, which supports the hypothesis that the low capacity of the Cl- homeostasis in immature neurons could be a substrate for synaptic scaling and homeostatic plasticity.

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“…Hippocampal CRc have been specifically labeled by expression of EYFP under the control of Wtn3a [75] or CXCR4 [5]. In the MZ of the developing cortex the tumor [79], respectively). e CRc stained with anti-reelin antibody in a tangential slice preparation of a P2 rat suppressor gene P73 is found exclusively in CRc [63].…”

Section: Excurse 1: Reelinmentioning

confidence: 99%

Cajal-Retzius cells: organizers of cortical development

Kilb

Frotscher²

2016

E-Neuroforum

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Electrophysiological and morphological properties of Cajal–Retzius cells with different ontogenetic origins

Cited by 31 publications

References 57 publications

Resonance properties of different neuronal populations in the immature mouse neocortex

Resonance properties of different neuronal populations in the immature mouse neocortex

Activity-dependent scaling of GABAergic excitation by dynamic Cl− changes in Cajal–Retzius cells

Cajal-Retzius cells: organizers of cortical development

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