Electrophoretic Study of Proteinuria in Toxemia of Late Pregnancy

Parviainen, S; Soiva, K; Ehrnrooth, C. A.

doi:10.3109/00365515109060616

Cited by 21 publications

(3 citation statements)

References 5 publications

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“…Studies of urinary protein in pregnancy have been largely confined to conventional methods (Mack, 1955;Parviainen et al, 1951;Lorincz et al, 1961). Attempts have been made to correlate electrophoretic urinary protein patterns with the nature of the underlying pathology (Glass er al., 1963).…”

Section: Discussionmentioning

confidence: 99%

Proteinuria in Pre‐eclampsia

Simanowitz

MacGregor

Hobbs

1973

BJOG

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Summary The urine/serum ratios of seven proteins of varying molecular weight have been determined in patients with pre‐eclampsia, using radial immunodiffusion. A linear excretion pattern of protein clearance on molecular weight has been confirmed in pregnancy proteinuria. Protein selectivity indices, based on this relationship have been derived for each patient with pre‐eclampsia and were found to lie in the intermediate range of selectivity.

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Section: Discussionmentioning

confidence: 99%

Proteinuria in Pre‐eclampsia

Simanowitz

MacGregor

Hobbs

1973

BJOG

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“…23 Absolute values for serum albumin and globulin are lower in toxemias than in uncomplicated pregnancies. 41 ' 93 Parviainen and coworkers 108 found all the plasma protein fractions, except fibrinogen, in the urine of patients with toxemias of pregnancy of varying severity. In mild toxemia, the percentage of albumin was lower and that of alpha globulin higher than in the plasma; in pre-eclampsia and eclampsia, the urinary albumin was higher and alpha globulin lower.…”

Section: Protein Patterns In Physiologic and Pathologic Statesmentioning

confidence: 99%

Recent Contributions of Electrophoresis to Clinical Pathology

Fisher

1953

Am J Clin Pathol

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Electrophoresis is one of several physicochemical methods for characterization of proteins. It is performed by passing a high-voltage current through a dilute solution or dispersion of the material to be examined and observing the migration of the charged particles. This technic characterizes a protein only in respect to its behavior within an electric field, under the specific conditions of the experiment. Certain other data may be obtained at the same time. It may be noted whether the material under analysis is "pure," or consists of several components; the isoelectric point of a substance may be determined by varying the pH. The speed of migration of the charged particles and their possible dissociation into components of different mobilities are dependent on the pH, ionic strength and specific conductivity of the solution and the field strength selected. Electrophoresis may be employed to isolate fractions of specific materials in a relatively pure form, as well as for their quantitative estimation.In 1937, Tiselius 166 improved the moving boundary apparatus and technic for electrophoresis (observation of the interface between the protein and a supernatant clear buffer of the same pH and conductivity) to the extent that readily reproducible results were obtained. The Tiselius apparatus was then used in many investigations to demonstrate specific changes in the protein patterns in certain disease processes. However, the abnormalities encountered were usually nonspecific, and great caution is advisable when attempting correlations between these patterns and specific pathologic conditions. Electrophoretic analysis is not necessary or desirable for routine clinical examination of body fluids for the following reasons: the high cost of the necessary equipment and specially trained personnel; the time required for the analysis of a specimen (only a few can be examined in one day); the need for photographic and developing procedures; and the necessity of drawing enlarged patterns for measurement of the relative areas. In most instances, accurate, rapid, chemical fractionation procedures are now available and preferable.7 ; 167 However, the terminology utilized for the major protein constituents of plasma is based on their electrophoretic differentiation.

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“…THE proteinuria of pre-eclampsia and eclampsia, placentae does not appear to have been studied which in this paper are called "toxaemia", has previously. been investigated by paper electrophoresis, Determinations of differential protein clearstarch gel electrophoresis, and by immuno-ance have proved more informative in the electrophoresis (Parviainen et al, 1951 ;Lorincz nephrotic syndrome than studies confined to the et al ., 1961 ;McEwan, 1968McEwan, , 1969Charvet et al, quantitative or qualitative assessment of urinary 1970). However, protein clearance determina-protein, and have been carried out increasingly tions have not been reported in detail in toxaemia.…”

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confidence: 99%

Proteinuria in Toxaemia and Abruptio Placentae

Maclean¹,

Wg²,

Smart

et al. 1972

BJOG

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Differential protein clearances were carried out in 31 patients with toxaemia of pregnancy and in 21 non-toxaemic patients with abruptio placentae. In 6 patients with toxaemia and in 7 with abruptio placentae, dextran clearance determinations were also performed. Results were expressed in terms of selectivity indices relating the clearances of these macromolecules to their molecular weights. In toxaemia, protein and dextran selectivity results were in the intermediate range, dextran values being slightly higher than the values obtained for protein. In abruptio placentae, however, the values obtained for protein selectivity were very low, while those obtained for dextran selectivity were very high. These results suggest that in toxaemia the proteinuria is of glomerular origin, but that in abruptio placentae the urinary protein is post-glomerular and is derived from plasma protein passing through damaged renal tubular epithelium.

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Electrophoretic Study of Proteinuria in Toxemia of Late Pregnancy

Cited by 21 publications

References 5 publications

Proteinuria in Pre‐eclampsia

Proteinuria in Pre‐eclampsia

Recent Contributions of Electrophoresis to Clinical Pathology

Proteinuria in Toxaemia and Abruptio Placentae

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