Electrophoretic study of Clostridium species

Cato, Elizabeth P.; Hash, D. E.; Holdeman, Lillian V.; Moore, W. E. C.

doi:10.1128/jcm.15.4.688-702.1982

Cited by 65 publications

(16 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolates produced acids in PYG broth in the following decreasing order of concentration: acetic, butyric, lactic, formic, and succinic. The electrophoretic pattern of proteins (Cato et al, 1982) of cells grown in BHI broth plus Tween and NaHCO, was similar for all isolates (data not shown). This electrophoretic pattern, when compared to previously described reference strains of Clostridium species (Cato et al, 1982), was most similar to C. t&urn.…”

Section: Resultsmentioning

confidence: 74%

Butyric Acid Spoilage of Fermented Cucumbers

Fleming

Daeschel

McFeeters

et al. 1989

Journal of Food Science

View full text Add to dashboard Cite

Small cucumbers brined to equalize at 2.3% NaCl in an anaerobic tank underwent a normal prim&y lactic acid fermentation that resulted in 1.09% titratable aciditv (as lactic) and oH 3.7. Nine months later the product was obsexvcb io have spoiled, as evidenced by an unpleasant odor. Products formed during spoilage in order of concentration were acetic acid > butyric acid > n-propanol > propionic acid. No lactic acid remained. No botulinal toxin was detected. Closrridiunz tertium was identified as contributing to the spoilage, but did not produce propionic acid or n-propanol under test conditions. Evidcnce indicated that unidentified bacteria, possibly propionibacteria sp., degraded lactic acid causing a rise in pH which allowed C. tertium to grow.

show abstract

Section: Resultsmentioning

confidence: 74%

Butyric Acid Spoilage of Fermented Cucumbers

Fleming

Daeschel

McFeeters

et al. 1989

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…This view is supported by the high degree of cultural, biochemical and SDS-PAGE similarity between six Australian isolates and two exotic strains of A. salmonicida isolated from goldfish. SDS-PAGE analysis of bacterial protein provides highly sensitive and reproducible phenotypic characterisation of bacteria (Cato, Hash.. Holeman & Moore 1982) and is a powerful technique for verifying the identity of bacteria (Moore, Hash, Holeman & Cato 1980). The similar protein profiles of the A. salmonicida strains indicated that the strains were very closely related.…”

Section: Discussionmentioning

confidence: 99%

Clinical, microbiological and epidemiological findings in recent outbreaks of goldfish ulcer disease due to atypical Aeromonas salmonicida in south‐eastern Australia

Whittington

Gudkovs²,

Carrigan

et al. 1987

Journal of Fish Diseases

View full text Add to dashboard Cite

The spread of goldfish ulcer disease (GUD) from Victoria to New South Wales, Austraha, and the first isolation oi Aeromonas salmonicida from wild goldfish are reported. Cultural, biochemical and protein SDS-PAGE characteristics of these recent isolates are compared with those of existing Australian isolates, with strains recovered from goldfish in Italy and the USA (atypical strains) and with strain ATCC 14174 (typical strain). The Australian isolates were identical and closely resembled the exotic atypical strains. Although there were several biochemical differences between the atypical isolates and the typical ATCC 14174 strain, the results of SDS-PAGE confirmed that these strains were closely related. The homology of the Australian and overseas strains recovered from goldfish supports the common view that A. salmonicida was introduced first into Australia with diseased goldfish in 1974. The three widely separated outbreaks of GUD reported here confirm that an atypical strain of A. salmonicida is now endemic in Austraha.

show abstract

“…Soluble protein profiles. The polyacrylamide slab gel electrophoresis procedure described by Moore et al (30) and Cato et al (5) was used to compare soluble cellular proteins from overnight cultures in brain heart infusion-Tween-carbonate broth (30). As controls, Streptococcus faecalis strain VPI U4-20 and C. difficile strains VPI 8491 (ATCC %89) and VPI 10463 were included.…”

Section: Louis Mo) (8)mentioning

confidence: 99%

Investigation of an outbreak of antibiotic-associated colitis by various typing methods

Wüst¹,

Sullivan²,

Hardegger³

et al. 1982

J Clin Microbiol

137

View full text Add to dashboard Cite

During an outbreak of diarrheal disease due to Clostridium difficile in a surgical ward, 16 C. difficile isolates were cultured from fecal samples of 15 patients. Agarose gel electrophoresis for the detection of plasmid DNA, crossed immunoelectrophoresis for the detection of extracellular antigens and toxins, polyacrylamide gel electrophoresis for analyses of soluble proteins, assays for cytotoxicity, and a comparison of susceptibility to antimicrobial agents were employed. At least 12 of the 16 isolates were shown to be phenotypically the same strain. These findings suggest that in a hospital setting, diarrhea and pseudomembranous colitis caused by C. difficile can be of nosocomial origin and that they can spread from patient to patient.

show abstract

Electrophoretic study of Clostridium species

Cited by 65 publications

References 14 publications

Butyric Acid Spoilage of Fermented Cucumbers

Butyric Acid Spoilage of Fermented Cucumbers

Clinical, microbiological and epidemiological findings in recent outbreaks of goldfish ulcer disease due to atypical Aeromonas salmonicida in south‐eastern Australia

Investigation of an outbreak of antibiotic-associated colitis by various typing methods

Contact Info

Product

Resources

About