1982
DOI: 10.1128/jcm.16.6.1096-1101.1982
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Investigation of an outbreak of antibiotic-associated colitis by various typing methods

Abstract: During an outbreak of diarrheal disease due to Clostridium difficile in a surgical ward, 16 C. difficile isolates were cultured from fecal samples of 15 patients. Agarose gel electrophoresis for the detection of plasmid DNA, crossed immunoelectrophoresis for the detection of extracellular antigens and toxins, polyacrylamide gel electrophoresis for analyses of soluble proteins, assays for cytotoxicity, and a comparison of susceptibility to antimicrobial agents were employed. At least 12 of the 16 isolates were … Show more

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Cited by 137 publications
(37 citation statements)
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“…spread of C. difficile, particularly when the organism is isolated from patients and their hospital environment (5, 6, 9), the need to establish a C. difficile typing method to elucidate the epidemiology of this organism remains. Several workers have initiated various schemes to investigate outbreaks of diarrhea associated with antibiotic therapy and chemotherapy (14, 19), and others have described various methods for typing the organism (2, 13, 16,17). In this paper we report a typing method based on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins.…”
mentioning
confidence: 99%
“…spread of C. difficile, particularly when the organism is isolated from patients and their hospital environment (5, 6, 9), the need to establish a C. difficile typing method to elucidate the epidemiology of this organism remains. Several workers have initiated various schemes to investigate outbreaks of diarrhea associated with antibiotic therapy and chemotherapy (14, 19), and others have described various methods for typing the organism (2, 13, 16,17). In this paper we report a typing method based on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins.…”
mentioning
confidence: 99%
“…The 630 strain was isolated in 1982, so passaging within and between labs could have led to accumulation of mutations that reduced the frequency of flagellar phase variation in 630. We thus asked whether the lack of (or very low frequency of) flagellar switch inversion in 630 is unique to this strain and its derivatives, or a broader attribute of currently circulating ribotype 012 C. difficile isolates (18). To test this, we evaluated flagellar phase variation in six riboype 012 isolates: SS235, a community environmental isolate; SE838, a hospital environmental isolate; and MAM30, MT4768, MT5065, and MT5066, isolates from patients with CDI (kindly gifted by Dr. Kevin Garey).…”
Section: Resultsmentioning
confidence: 99%
“…For C. difficile strains R20291, a ribotype 027 strain, and ATCC 43598, a ribotype 017 strain, both the flg ON and OFF orientations were apparent under multiple conditions tested. However, only the flg ON orientation was detectable in 630, a ribotype 012 strain originally isolated from a patient with C. difficile infection (18). However, recent work from Collery et al suggests the flagellar switch is capable of inversion in 630 (19), suggesting this strain is phase-locked.…”
Section: Introductionmentioning
confidence: 99%
“…TcdB2 has enhanced ability to enter host cells, is more cytotoxic, and exhibits wider tissue tropism (44, 45). In contrast to RT027 strains, the PaLoc of RT106 strains is 100% identical to 630 (53-56); thus, these strains encode full-length TcdC and express the TcdB1 toxin variant. Both RT027 and RT106 isolates produce variable amounts of TcdA/TcdB.…”
Section: Discussionmentioning
confidence: 99%