Electrophoretic protein profile of nodulated and non-nodulated Cassia species in relation to taxonomy

Todaria, N. P.; Nautiyal, A. R.; Semwal, J.K.

doi:10.1016/0305-1978(83)90057-1

Cited by 4 publications

(5 citation statements)

References 5 publications

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“…8), allowing comparison with data from the RAPD analysis in this study. The similarity values determined by CENSTAT analysis were identical to those given by Todaria et al (1983). While samples now classified as Chamaecrista and Senna appear to be only loosely grouped, both lie within the range of values obtained for the species by RAPD analysis ( (1983).…”

Section: Molecular S E P a R A T I O N Of G E N E R A Of C A S S L I supporting

confidence: 74%

“…1). Todaria et al (1983) investigated seed proteins from a group of seven Cassiinae covering the three sub-genera and including nitrogen-fixing and non-fixing species. They expressed the degree of relatedness of pairs of species as percentage coefficient of similarity.…”

Section: Molecular S E P a R A T I O N Of G E N E R A Of C A S S L I mentioning

confidence: 99%

“…The protein gel electrophoresis results given by Todaria et al (1983) have been subjected to computer analysis using principal co-ordinate analysis (Fig. 8), allowing comparison with data from the RAPD analysis in this study.…”

Section: Molecular S E P a R A T I O N Of G E N E R A Of C A S S L I mentioning

confidence: 99%

“…Coefficient of similarity (%) found within genera by RAPD analysis and byTodaria et al (1983) for seed protein…”

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confidence: 99%

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Molecular separation of genera in Cassiinae (Leguminosae), and analysis of variation in the nodulating species of Chamaecrista

Whitty

Powell²,

Sprent

1994

Molecular Ecology

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Randomly Amplified Polymorphic DNA (RAPD) methods have been adapted for use as a phenetic tool on the legume tribe Cassiinae. RAPD-generated polymorphism within local populations was lower than between populations from different geographic regions, between species and genera. Examination of three Cassia species, 12 Chamaecrista species and 13 Senna species using eight primers showed the potential for separation of the nodulated/nitrogen fixing genus Chamaecrista from the previously congeneric groups Cassia and Senna. Similarly, RAPD analysis of two groups of nine Ch. rotundifolia and nine Ch. mimosoides samples using 11 primers has given separation according to both species and to geographical location. Analysis of a small sample of five Chamaecrista species from Brazil with eight primers gave separation consistent with known variations in nodule structure.

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Section: Molecular S E P a R A T I O N Of G E N E R A Of C A S S L I supporting

confidence: 74%

Section: Molecular S E P a R A T I O N Of G E N E R A Of C A S S L I mentioning

confidence: 99%

Section: Molecular S E P a R A T I O N Of G E N E R A Of C A S S L I mentioning

confidence: 99%

“…Coefficient of similarity (%) found within genera by RAPD analysis and byTodaria et al (1983) for seed protein…”

mentioning

confidence: 99%

See 2 more Smart Citations

Molecular separation of genera in Cassiinae (Leguminosae), and analysis of variation in the nodulating species of Chamaecrista

Whitty

Powell²,

Sprent

1994

Molecular Ecology

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“…We used 25 mg of sample instead of 10 mg, and 500 μL of extraction buffer solution instead of 200 μL. The resulted peroxidase isozymes banding pattern were then calculated for their Rf values using formula of Todaria et al (1983). All activities of electrophoretic isozyme of peroxidase were run at our laboratory of molecular biology-FMIPA UNS, from June to August 2014.…”

Section: Electrophoresis Of Peroxidase Isozymesmentioning

confidence: 99%

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

2019

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Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars and variations in the morphological characters not only for the leaves but also for the tubers. Sweet potatoes had economically significant role, especially as an additional food due to its high content of carbohydrate and other nutrient values. In order to test whether the morphological variation in this species was also expressed at the micro character; such as pollen morphology and enzyme activity, ten sweet potato cultivars were used in this study. The morphological characters such as shape and structure of the pollen features were examined using Scanning Electron Microscopy (SEM). Accordingly, Polyacrylamide Gel Electrophoresis (PAGE) was used to look-at the banding pattern of peroxidase isozymes. Morphological characters of leaves, flowers, and tuber were observed qualitatively both for the typical shape and colors. More detail of flower shapes was drown manually especially their petal edges. The results showed that there was variation among the ten sweet potato cultivars confirmed not only on their morphological characters but also the isozyme banding patterns of ten cultivars tested. At least, two cultivar Antin 3 and Papua Solossa had uniques characters especially the purple stem and yellow tuber and dark skin on the tuber, respectively. In addition, the structural analysis of pollen showed that Beta 1 cultivar has very large pollen; 116,50 μm (axis polar) and the contour surface of exine pollen was concave with the aperture shape and ornamentation were prolate spheroidal and echinate respectively. This unique character was also shown by its peroxidase isozyme bands. Eleven bands of leaf and 13 bands of stem samples were detected respectively. The typical Rf values of 0.31, 0.48 and 0.41 were detected for the leaf, while for stem samples were 0.14, 0.20 and 0.26. The Beta 1 cultivar showed very thick bands in between Rf values of 0.13 and 0.26. Meanwhile, the unique band pattern of Papua Salosa was the absence of band at 0.38.

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Evolution and Classification of Seed Plants

Poppendieck

1985

Progress in Botany

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Electrophoretic protein profile of nodulated and non-nodulated Cassia species in relation to taxonomy

Cited by 4 publications

References 5 publications

Molecular separation of genera in Cassiinae (Leguminosae), and analysis of variation in the nodulating species of Chamaecrista

Molecular separation of genera in Cassiinae (Leguminosae), and analysis of variation in the nodulating species of Chamaecrista

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

Evolution and Classification of Seed Plants

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