Electrophoretic karyotyping of typical and atypical Candida albicans

Abstract

Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), were compared with those of typical C. albicans. Karyotypes of whole-cell DNA of classical C. albicans examined with transverse alternating-field electrophoresis under specific conditions were composed of seven DNA bands with a specific migration pattern. Certain atypical strains and representatives of the three serotypes of C. stellatoidea produced discrete karyotypes with 5 to 10 bands. All isolates demonstrated a significant degree of DNA relatedness, suggesting their conspecificity. Densitometric tracings of DNA bands provided an objective and standardized method for comparing bands within the gels.

Systemic neonatal candidosis: the karyotyping of Candida albicans strains isolated from neonates and health-workers

“…[5][6][7][8][9] Our results are in good agreement with those reported in previous studies on PFGE typing of C. albicans where the karyotype pattern consists mainly of six or seven bands, but more rarely eight bands. [4][5][6][7][8][9][10][11][12][13][14][15][16][17] Differences in the patterns obtained may be related to technical variations in PFGE technique used. [7][8][9][10]17 It is important to point out that the number of bands revealed by PFGE does not necessarily correspond to the number of the yeast chromosomes.…”

“…[4][5][6][7][8][9][10][11][12][13][14][15][16][17] Differences in the patterns obtained may be related to technical variations in PFGE technique used. [7][8][9][10]17 It is important to point out that the number of bands revealed by PFGE does not necessarily correspond to the number of the yeast chromosomes. Indeed, homologous chromosomes may migrate separately possibly because of deletion, translocation, insertion of movable DNA or differences in the amount or distribution of repetitive sequences.…”

Phenotypic and genotypic characterization of oral yeasts from Finland and the United States

“…Pheno-or genotypically atypical C. albicans isolates have also been described in previous studies. Mahrous et al (26) found distinct karyotypes among atypical C. albicans isolates (isolates that had at least one of the following characteristics: germ-tube negative, saccharose-negative, no proteinase activity, low virulence for mice, formation of hyperchlamydocinidia or hyperhyphae) compared to karyotypes of typical C. albicans isolates. McCullough et al (28) found that some phenotypically normal C. albicans isolates from HIVinfected subjects did not hybridize to a species-specific probe and exhibited higher extracellular proteinase activity, greater adherence to buccal epithelial cells and lower susceptibity to 5-flucytosine than the typical C. albicans isolates.…”

Characterization of Candida krusei strains from spontaneously fermented maize dough by profiles of assimilation, chromosome profile, polymerase chain reaction and restriction endonuclease analysis

“…Until recently, most of the characterization of Candida spp. has been concentrated on C. albicans (Iwaguchi et al 1990;Mahrous et al 1990;Magee et al 1992). Recently, two © 1999 The Society for Applied Microbiology, Journal of Applied Microbiology 87, 29-40 groups of workers (Carlotti et al 1994(Carlotti et al , 1996(Carlotti et al , 1997aManavathu et al 1996) have used variations of molecular methods for typing of clinical strains of C. krusei.…”