Electrophoretic Investigations of a Clone of Hamster Fibroblasts and Polyoma-Transformed Cells from the Same Population

The electrophoretic mobility of normal and leukaemic cells of mice

“…Many studies have been made in an attempt to elucidate the nature of cell-surface ionogenic groups. The view that ionizable phosphate groups, probably associated with a complex phospholipid system, are mainly responsible for the electrokinetic properties of cells (Winkler & Bungenberg de Jong, 1941) must now be revised, since more recent studies Eylar, Madoff, Brody & Oncley, 1962) have demonstrated that sialic acid anions are responsible for a major portion of the surface negative charges on erythrocytes and various tumour cells (Cook, Forrester, Ambrose & Macpherson, 1962).…”

Cell surfaces.

“…Similarly, measurements of Ehrlich ascites tumour cells, even when effected in various phases of mitosis, showed a very small scatter in mobility values (Cook, Heard and Seaman, 1962). Furthermore, as demonstrated by Forrester, Ambrose and Macpherson (1962), polyoma induced malignant cells manifested an increased charge when compared with normal cells with the same rate of growth.…”

Neoplastic Transformation by Polyoma Virus and its Wider Implications

“…Crossing of the cells in an uncrowded plate is rare through the effect of contact inhibition (Abercrombie and Ambrose, 1962). Transformed or tumour cells are often triangular and they lie at random, criss-crossing one another and piling up several layers thick, presumably owing to loss of (Forrester et al, 1962) (Macpherson, 1963). Normal cells can sometimes be transplanted successfully if large numbers (more than one million) are injected into hamsters, but they grow slowly.…”