Electrophoretic fractionation of serum γ-glutamyl transpeptidase in human hepatic cancer

淳之介, 児島; 正子, 金谷; 允人, 中村; 徹, 柏木; 雅彦, 秋山; 道夫, 加藤; 美子, 樽井

doi:10.2957/kanzo.21.866

Cited by 7 publications

(12 citation statements)

References 4 publications

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“…Kojima et al. found that the specific GGT isoenzyme could be detected in 55% of patients with HCC, and concluded that the isoenzyme was useful in diagnosis of HCC 14 . Sawabu et al.…”

Section: Discussionmentioning

confidence: 99%

Clinical application of an enzyme‐linked immunosorbent assay detecting hepatoma‐specific γ‐glutamyltransferase

Wang¹,

Zhang²,

Zhao³

et al. 2009

Hepatology Research

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“…Kojima et al. found that the specific GGT isoenzyme could be detected in 55% of patients with HCC, and concluded that the isoenzyme was useful in diagnosis of HCC 14 . Sawabu et al.…”

Section: Discussionmentioning

confidence: 99%

Clinical application of an enzyme‐linked immunosorbent assay detecting hepatoma‐specific γ‐glutamyltransferase

Wang¹,

Zhang²,

Zhao³

et al. 2009

Hepatology Research

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“…However, it has become clear that there are several different core proteins for each major type of proteoglycan and that different cell types express different proteoglycans (1,4,6,12,13). In addition, there may be differences in proteoglycan expression between normal and neoplastic cells (14)(15)(16).…”

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confidence: 99%

Molecular cloning and sequence analysis of a chondroitin sulfate proteoglycan cDNA.

Bourdon¹,

Oldberg²,

Pierschbacher³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

185

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We report the identification and DNA se- Synthesis of cDNA. Double-stranded cDNA was synthesized by using oligo(dT) (P-L Biochemicals) for priming first strand synthesis. Avian myeloblastosis virus reverse transcriptase (Life Sciences, St. Petersburg, FL) was used for synthesis of both strands (25). S1 nuclease-treated cDNA was ultracentrifuged on a 5-20% sucrose gradient and cDNA between 600 and 4000 base pairs (bp) was collected for dCtailing. Poly(dC)-tailing of the 3' cDNA termini was done by using terminal deoxynucleotidyl transferase (New England Nuclear). The dC-tailed cDNA was annealed to Pst I-restricted dG-tailed pBR322 (New England Nuclear) (26) and used to transform Escherichia coli K-12 strain MM294 (endol rkMj Blpro-) by the procedure of Kushner (27).Synthesis of Mixed Oligonucleotides. Oligonucleotides were synthesized by the solid-phase phosphotriester method as described (28,29). The oligonucleotides were purified by HPLC (30) on an RP-C18 reversed phase column (Bio-Rad). Radiolabeled oligonucleotides were prepared by the 5' addition of 32p using [-32P]ATP (3000 Ci/,umol; 1 Ci = 37 GBq; New England Nuclear) and T4 polynucleotide kinase (New England Nuclear) (29).Screening of cDNA Clones. Recombinant cDNA clones were selected on LB agar plates supplemented with 12.5 ,ug of tetracycline per ml. Nitrocellulose replicas of the cDNA library were prepared and replica clones were screened by in situ hybridization with 32P-labeled mixed oligonucleotides (29,31). Plasmid DNAs prepared as described (32) from clones hybridizing with the probe oligonucleotides were further screened and insert size was determined by Southern hybridAbbreviation: bp, base pair(s). 1321The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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“…Neither glycosaminoglycan synthesis nor secretion in cultures of parenchymal and other non-parenchymal cells were evaluated under comparable conditions. Information on the relative contribution of the various cell types to hepatic proteoglycan and hyaluronate synthesis will be important since under pathological conditions like liver fibrosis (25)(26)(27)(28) and hepatocellular carcinoma (29,30), both the total glycosaminoglycan content and the pattern of suiphated and non-sulphated glycosaminoglycans chanige greatly. In the present study we attempted to compare the synthesis, secretion, and molecular profile of glycosaminoglycans of hepatocytes, fat-storing cellSj and Kupffer cells kept in culture under largely identical conditions.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Sulphated Glycosaminoglycan and Hyaluronate Synthesis and Secretion in Cultured Hepatocytes, Fat Storing Cells, and Kupffer Cells

Gressner¹,

Schäfer²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:The extracellular matrix of normal liver contains several types of proteoglycans including heparan sulphate, chondroitin sulphate isomers, dermatan sulphate, and the glycosaminoglycan, hyaluronic acid. In the present study both the synthesis and secretion äs well äs the pattern of radioactively labeled proteoglycans and hyaluronic acid of hepatocytes, fat-storing cells (Ito cells), and Kupffer cells maintained in monolayer cultures under mostly identical conditions were compared to assess their relative contribution to hepatic proteoglycan synthesis. Fat-storing cells were identified äs the main type of cell producing and secreting proteoglycan and hyaluronic acid. More than 70% of labeled proteoglycan and hyaluronic acid were secreted into the medium. Heparan sulphate is the main type of proteoglycan in hepatocytes, whereas in the medium of fat-storing cells, chondroitin sulphate and dermatan sulphate comprise the mäjor fractions. Hyaluronic acid was not detectable in hepatocyte cultures and found only in low amounts in the medium of Kupffer cells. The results point to a stringent quantitative and qualitative cellular compartmentation of proteoglycan synthesis in liver with fat-storing cells äs the most important cell type for matrix proteoglycan and hyaluronic acid production.

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Electrophoretic fractionation of serum γ-glutamyl transpeptidase in human hepatic cancer

Cited by 7 publications

References 4 publications

Clinical application of an enzyme‐linked immunosorbent assay detecting hepatoma‐specific γ‐glutamyltransferase

Clinical application of an enzyme‐linked immunosorbent assay detecting hepatoma‐specific γ‐glutamyltransferase

Molecular cloning and sequence analysis of a chondroitin sulfate proteoglycan cDNA.

Comparison of Sulphated Glycosaminoglycan and Hyaluronate Synthesis and Secretion in Cultured Hepatocytes, Fat Storing Cells, and Kupffer Cells

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