Electrophoretic forms of lysozyme activity in various plant species

Audy, Patrice; Quéré, Dominique Le; Leclerc, Denis; Asselin, Alain

doi:10.1016/0031-9422(90)85419-g

Cited by 18 publications

(2 citation statements)

References 37 publications

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“…Lysozyme activity was assayed as described by Audy et al (1990) by using the lyophilized cells oi Micrococcus lysodeiklicus as the substrate; lysozyme purified from chicken egg white (Sigma) was used as a positive control, Antiftmgal activity was tested oo Trkhoderma sp. as described by Zhang and Puoja (1994).…”

Section: Lysozyme and Antifnngal Activitiesmentioning

confidence: 99%

Chitinase profiles in mature carrot (Daucus carota) roots and purification and characterization of a novel isoform

Zhang¹,

Haunerland²,

Punja³

1996

Physiol Plant

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mature carrot (Daueus carota) roots and purification and characterization of a novel isoform. -Physiol. Plant. 96: 130-138,The profile of cMtinases (EC 3.2.1,14) in mature carrot {Daucus carota L. cv. Eagle) roots was studied. Multiple chitinase bands (8-10) were observed in native and sodium dodecylsulfate-denaturing polyacrylamide gels. The molecular masses of these chitinases were estimated to be from 20 000 to 40 000. One major chitinase was purified and found to be an acidic protein with pi at 4,3 and a molecular mass of 39 500. The optimum pH for enzymatic activity was around 5 and the optimum temperature was 25°C, The enzyme was stable at pHs below 8 and temperatures below 60°C. The protein did not have a chitin-binding domain, but showed some similarity to the amino acid composition of tobacco class I chitinase. The N-terminal amino acid sequence did not resemble any ofthe described classes of chitinases. This chitinase did not possess lysozyme activity and showed antifungal activity when tested against Trichoderma sp.

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Section: Lysozyme and Antifnngal Activitiesmentioning

confidence: 99%

Chitinase profiles in mature carrot (Daucus carota) roots and purification and characterization of a novel isoform

Zhang¹,

Haunerland²,

Punja³

1996

Physiol Plant

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“…The appearance of lysozyme is described in various plant tissues like roots, leafs, flowers, seeds, fruits and latices (Audy et al, 1990). Several lysozymes from plant latices have been reported like Hevea brasiliensis (Willd.…”

Section: Lysozymementioning

confidence: 99%

Lysozyme- and chitinase activity in latex bearing plants of genus Euphorbia – A contribution to plant defense mechanism

Sytwala

Günther

Melzig

2015

Plant Physiology and Biochemistry

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Use of hen lysozyme for protection against bacterial contamination ofin vitro embryo cultures

et al. 1995

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Curative treatments with antibiotics and hen egg white lysozyme (HEWL) were used to salvage embryo cultures contaminated withBaccillus subtilis. The use of HEWL gave good control ofBaccillus subtilis, but no control ofErwinia. HEWL was better than antibiotics, being much less phytotoxic. The antibiotics piperacillin, ampicillin and imipenem were also found to be ineffective againstErwinia. HEWL, at a final concentration of 1 mg per mL, was used as a preventive and curative agent for routine use in embryo culture ofTriticum aestivum and other Triticeae, as it cured from 30% to 50% of bacterial contamination problems over a one year period. Standardin vitro culture precautions remained essential, as certain bacteria were not controlled by HEWL.

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Electrophoretic forms of lysozyme activity in various plant species

Cited by 18 publications

References 37 publications

Chitinase profiles in mature carrot (Daucus carota) roots and purification and characterization of a novel isoform

Chitinase profiles in mature carrot (Daucus carota) roots and purification and characterization of a novel isoform

Lysozyme- and chitinase activity in latex bearing plants of genus Euphorbia – A contribution to plant defense mechanism

Use of hen lysozyme for protection against bacterial contamination ofin vitro embryo cultures

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