Electrophoretic fingerprinting and the biological activity of colloidal indicators

Marlow, Bruce J.; Fairhurst, David; Schütt, Wolfgang

doi:10.1021/la00081a050

Cited by 20 publications

(2 citation statements)

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“…Since each run was carried out over a very narrow range of conductivity, it was possible to obtain a 2-dimensional, statistically obtained, profile of U em as a function of pH at approximately constant pλ using the wireframe methodology in the SURFER program. This approach is the first use of the 2-dimensional profile to examine the dynamics on a growing cell system but it is reminiscent of the early work on EF where electrophoretic mobility distributions were used in the work on the use of latex particles as biological indicators …”

Section: Methodsmentioning

confidence: 99%

Microbicides for HIV/AIDS. 1. Electrophoretic Fingerprinting the H9 Cell Model System

Rowell

Fairhurst²,

Morfesis³

et al. 2005

Langmuir

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An electrophoretic fingerprint of a CD4+ T-cell (H9) has been produced for the first time. Samples were taken from three separate cultures prepared at different times to obtain a general characterization of the cells. The availability of commercial instrumentation equipped with an auto-titrator has made possible the application of both the 2-dimensional and 3-dimensional representation of electrophoretic fingerprinting. The 2-dimensional treatment has been used to assess the reliability of the data and has detected hysteresis as a possible second-order effect. The 3-dimensional representation has been used to explore the data needed for a reliable overall pattern that characterizes the conditions of pH and conductivity required for an effective microbicide. The dome negative maximum in the electrophoretic fingerprint at high pH, along with the line of zero mobility (LZM) and a dome positive maximum at low pH, are interpreted as evidence for surface carboxyl groups prominent in the alkaline regime and surface amino groups prominent in the acid regime, suggesting that the H9 cell surface is zwitterionic. This has important implications as to the choice and design of microbicide actives.

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Section: Methodsmentioning

confidence: 99%

Microbicides for HIV/AIDS. 1. Electrophoretic Fingerprinting the H9 Cell Model System

Rowell

Fairhurst²,

Morfesis³

et al. 2005

Langmuir

Self Cite

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“…Generally, electrophoretic measurements can be carried out with automated techniques based on single particle measurements by microscopic image tracking and analysis ( 16), image transduction techniques, or laser Doppler effect techniques. To improve the value of electrokinetic studies, the method of electrophoretic fingerprinting was introduced to analyze different batches of polymer carriers ( 17). Electrophoretic fingerprints or templates are three‐dimensional representations of the mean electrophoretic mobility of a given particle suspension versus the pH and ionic strength of the media.…”

Section: Principles Of Particle Characterization By Magnetophoresis Amentioning

confidence: 99%

Biocompatible Magnetic Polymer Carriers for In Vivo Radionuclide Delivery

Schütt

Grüttner²,

Teller³

et al. 1999

Artificial Organs

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The magnetic guidance of antiplastic and antibacterial agents as well as x-ray and MRI contrast substances in vivo by means of magnetic particles has been attempted repeatedly during the last 2 decades with more or less success. When using microparticles, the circulation time in the blood, the biodistribution, and to a greater or lesser extent, the specific targeting are determined by the uniformity of size, chemical composition, surface modification, and the electric surface charge. The electrophoretic mobility is an important parameter for the prediction of the usefulness of the prepared particle, modified by chemical and biological molecules. For its success, radionuclide therapy depends on the critical relationship between the amount of radioactive isotopes in the target tissue and in critical normal tissue. Because the implementation of radioimmunotherapy for the treatment of cancer has proven to be considerably more difficult than initially anticipated, we propose the use of magnetic nanospheres for the well directed delivery of radionuclides to a tumor after the intravenous administration of the biodegradable colloidal suspension.

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Biomedical and clinical applications of automated single cell electrophoresis

et al. 1990

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The automated single cell electrophoresis microscope Parmoquant has been used for the discrimination of lymphocytes and the study of the interaction of substances with cells and synthetic particles. Electrophoretic histograms allowed the determination of changes in the proportion of lymphocyte populations after kidney transplantation, during dialysis treatment, open heart surgery and during pregnancy. Discrimination of leukemic cells on the basis of electrophoresis was used as an additional parameter in diagnosis. In a mouse tumor model, histogram determination enabled the in vivo effect of the tumor necrosis factor on immune cells to be evaluated. Cell electrophoresis was shown to be suitable to detect the influence of antibodies, lectins and bacteria on the cell surface. Protein adsorption was studied on synthetic particles using cell electrophoresis. This method was applied to investigate the phenomena of blood interaction with biomaterials used in artificial organs and to determine differences in the protein composition of serum or other body fluids connected with diseases.

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Electrophoretic fingerprinting and the biological activity of colloidal indicators

Cited by 20 publications

References 0 publications

Microbicides for HIV/AIDS. 1. Electrophoretic Fingerprinting the H9 Cell Model System

Microbicides for HIV/AIDS. 1. Electrophoretic Fingerprinting the H9 Cell Model System

Biocompatible Magnetic Polymer Carriers for In Vivo Radionuclide Delivery

Biomedical and clinical applications of automated single cell electrophoresis

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