Electrophoretic Characteristics of Nuclear Proteins from Human Spermatozoa

Lescoat, D.; Colleu, D.; Boujard, Daniel; Lannou, D. Le

doi:10.3109/01485018808987050

Cited by 17 publications

(7 citation statements)

References 9 publications

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“…In this way our experiments supplement the results of Lescoat et al (1988). In the case of the examination of sperm nucleoproteins, various falsifications are possible by aggregations or proteolysis of proteins, and further by contamination with other proteins than sperm nucleus non-protamines.…”

Section: Discussionsupporting

confidence: 73%

“…Examinations of nucleoproteins of single ejaculates revealed very different electrophoretic patterns: some semen samples showed an electrophoretic distribution of spermatozoa1 nucleoproteins as described by other authors (Puwaravatipanich and Panyim -1975;Pongsawasdi and Svasti -1976;Tanphaichitr et al -1982;Lescoat et al -1988): Protamines HPS 1/2, H P 1, HP 2, H P 3, HP 4 and afew slight bands like histones. Other semen samples hardly showed protamines whereas more bands could be seen which were slower than HPS 1, the non-protamines.…”

Section: Electrophoretic Patterns Of Nucleoproteins Of Various Semen supporting

confidence: 61%

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Electrophoretic patterns of spermatozoal nucleoproteins (NP) in fertile men and infertility patients and comparison with NP of somatic cells

et al. 2009

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Summary During spermatogenesis in mammals the evolutionary early histones are replaced step by step by more species specific nucleoproteins (protamines). The protamines protect the genetic information of the male against mutagenic or other damaging influences and possibly play a role in male fertility. A method was presented to study the nucleoproteins (NP) of single human ejaculates. This procedure was checked concerning its usability and validity by bull spermatozoa. The NP of cryopreserved human spermatozoa of fertile men and of infertility patients were investigated and compared with the electrophoretic patterns of NP of somatic hepatocytes of rat and mouse. A total of 48 semen samples were studied. Ten of these samples were obtained from semen donors of proven fertility and 38 samples were collected from male partners of infertile couples. Photodensitometrically it could be distinguished between percentages of non‐protamines (PNP) and percentages of protamines (PP). The PP varied intra‐individually with a mean standard deviation of 34%, analogously to the alterations of classical semen parameters. The PP of fertile men did not significantly differ from the infertility patients with normal spermiogram parameters but showed a significantly higher value compared with the semen samples with pathological spermiograms. On average the PP of all infertile patients were significantly lower than the PP of fertile semen donors. Zusammenfassung Während der Spermatogenese werden die Histone schrittweise durch die speziesspezifischen Nukleoproteine (Protamine) ersetzt. Die Protamine tragen durch ihre Wechselwirkung mit der DNA zur kristallähnlichen Chromatinstruktur und somit zum Schutz der genetischen Information des Mannes bei. In der vorliegenden Arbeit wurde eine Methode zu Untersuchung der Nukleoproteine von Einzelejakulaten vorgestellt und mit Bullenspermien auf ihre Vergleichbarkeit und Validität überprüft. Das Nukleoproteinmuster von Humanspermien kryokonservierter Spermaproben von fertilen Männern und Infertilitätspatienten wurde elektrophoretisch untersucht und mit der Nukleoproteinverteilung von Ratten‐ und Mäusehepatozyten als Beispiel somatischer Zellen verglichen. 10 Spermaproben von sicher fertilen Spermaspendern und 38 Proben von männlichen Partnern infertiler Paare wurden berücksichtigt. Photodensitometrisch konnte zwischen dem Prozentanteil der Nichtprotamine (PNP) und der Protamine (PP) unterschieden werden. Die PNP variierte intraindividuell mit einer Standardabweichung von 34% analog zur Variation der klassischen Spermiogrammparameter. Der PP sicher fertiler Männer unterschied sich nicht signifikant von denen der Infertilitätspatienten mit normalen Spermiogrammparametern, zeigte aber einen signifikant höheren Wert als den PP der Spermaproben mit pathologischen Spermiogrammen. Der PP aller Infertilitätspatienten lag signifikant unter dem PP fertiler Spermaspender.

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Section: Discussionsupporting

confidence: 73%

Section: Electrophoretic Patterns Of Nucleoproteins Of Various Semen supporting

confidence: 61%

Electrophoretic patterns of spermatozoal nucleoproteins (NP) in fertile men and infertility patients and comparison with NP of somatic cells

et al. 2009

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“…In man, male infertility has been associated with both decreased levels of protamines (Silvestroni et al 1976;Chevaillier et al 1987;Lescoat et al 1988;Blanchard et al 1990;Sakkas et al 1996;Ziyyat et al 1999) and increased Prm1-to-Prm2 ratios Belokopytova et al 1993). Recently, Steger et al (2001) compared the fertilizing capacity of spermatozoa gained by testicular sperm extraction (TESE) and followed by intracytoplasmic sperm injection (ICSI) with the percentage of round spermatids in the corresponding biopsy exhibiting Prm1-mRNA and Prm2-mRNA.…”

mentioning

confidence: 99%

Haploid spermatids exhibit translationally repressed mRNAs

Steger¹

2001

Anatomy and Embryology

133

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During spermiogenesis, DNA-binding histones are replaced by protamines. DNA-protamine-interactions result in chromatin condensation causing cessation of transcription in elongating spermatids. Thus, in haploid spermatids, processes of transcription and translation need to be temporally uncoupled in order to ensure protein synthesis in transcriptionally silent germ cells. Post-transcriptional events, such as processing, transport, and storage of mRNAs, therefore, play important roles in determining when transcripts become functionally available for translation. This review focuses on mechanisms of translational regulation in haploid spermatids and male infertility caused by defects of translational control processes.

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“…Anomalies in the protamine content in infertile patients were already described more than 20 years ago [12,[154][155][156]. Subsequently, studies further confirmed the association of abnormal protamine content with abnormal seminal parameters and male infertility [14,15,21,35,[157][158][159][160][161][162][163][164][165].…”

Section: Protamine Anomalies In the Sperm Cells Of Infertile Patientsmentioning

confidence: 53%