Electrophoretic Behavior of Amylase in Mouse: The Parotid Gland is Major Source of Serum Amylase

Abstract

Amylase activity in the saliva, salivary glands, serum, liver (perfused and non perfused) and pancreas was assayed and isoamylases were separated by electrophoresis in these organs using C57BR/cdJ and M . m, molossinus (Kor) mice. Amylase isozymes in the saliva, parotid gland, serum and liver were identical in both strains , respectively. Amylase activity in the liver was lower than that in the serum and liver amylase disappeared almost by perfusion.Major serum amylase was released from the parotid gland in intact animals.In the mouse, a-amylase (EC 3.2.1.1.) is one of the major secretory enzymes of the salivary glands and of the pancreas. Many studies have appeared on salivary and pancreatic amylase [1, 11] using not only mouse but also rats and human.The parotid gland is an organ which has the highest amylase activity [7]. We previously reported that parasympathomimetic and sympathomimetic agents affected rat parotid gland in two ways : stimulation of amylase secretion into the saliva and acceleration of amylase release into the blood [6]. Though amylase activity in the serum of rat is lower than that in the parotid gland, a constant activity is detected in normal rat. Amylase isozyme in the human serum were detected in two types : parotid-and pancreas-type by electrophoresis [10].The increased activity of salivary isoamylase in human serum was seen with inflammatory disturbances of the salivary glands.In rat serum, the majority of isoamylase is parotid-type amylase [7]. Administration of pilocarpine increased the parotidtype amylase in rat serum significantly but this increase was abolished in parotidectomized rat [8]. This result suggested that the origin of serum amylase in rat was the parotid gland . Amerongen et al. [1] showed that the parotid amylase of mouse was partially identical to serum amylase by immunochemical studies . Arglebe, Bremer and Chilla [2] reported that activities of two salivary isoamylases in rat serum were increased by daily intraperitoneal injection of isoprenaline.These results indicated clearly that the source of serum amylase was the parotid gland after stimulation by drugs or inflammation.In this report we describe the electrophoretic behavior of amylase in mice (C57BR/ cdJ and MM m. molossinus (Kor)) which have different electrophoretic forms respectively and describe the source organ of serum amylase in intact mouse. Materials and MethodsMale and female C 57 BR/cdJ mice were obtained from Charles River Japan Inc., (Japan) and Mus Musculs molossinus (Kor)

The Embryo Pancreas is a Source of Increased Yolk Amylase in the Fertilized Eggs of Domestic Fowls

“…The homogenates were used to measure the amylase and to separate isoamylases by gel electrophoresis. The method of assaying amylase activity has been described [1]. All data were statistically analyzed by Student's t test.…”