Electrophoretic Assembly of Antibody–Antigen Complexes Facilitates 1000 Times Improvement in the Limit of Detection of Serological Paper-Based Assay

Panferov, Vasily G.; Ivanov, Nikita A.; Brinc, Davor; Fabros, Anselmo; Krylov, Sergey N.

doi:10.1021/acssensors.3c00130

Cited by 8 publications

(6 citation statements)

References 48 publications

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“…This blocking mechanism was reached via electrokinetically to drive delivery of the label once the antigen-antibody is in the test zone. The limit of detection using electrophoretically driven LFIA (eLFIA), has been proven to be 1000 times lower than that of conventionally LFIA [55]. Various post-assay upgrades based on chemical reactions to facilitate high sensitivity for LFIA have been presented in a critical review [56].…”

Section: Advanced Technologies To Enhance Lateral Flow Assay Analytic...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

Preprint

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Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage of formation, these maladies can be effectively treated, leading to successful outcomes; curative therapies can halt illnesses from advancing, thus improving the quality of life, and long-term survival of the patient. Still cutting-edge upgrades in precision technologies are necessary for early, reliable, affordable, and rapid disease detection, but also are vital for the well-being of people and the future of global public health. The emerging role and utility of non-invasive and repeatable diagnostic test approaches for detection of health conditions has been liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator or signature (e.g., proteins), to provide valuable information on individual’s wellness and/or disease. Among the bodily secretions used for non-invasive diagnostics is sputum, a complex viscous gel-like biopolymeric network, that has gained growing recognition as a rich source of biomarkers of airway infections, pulmonary diseases, and serves as a determinant to reveal other illnesses. As per the World Health Organization, the burden of respiratory conditions is exacerbated by factors, ranging from considerable subjection to air pollution and occupational contaminants, to tobacco smoking and second-hand smoke, in addition to poor socio-economic status. Due to the likely increase of these determinants, respiratory tract ailments are on the rise, affecting the health of many individuals, in addition to putting stress on healthcare facilities and services worldwide. We therefore highlight the need to use expectorated or induced sputum specimens as a routine sample source for testing valuable protein biomarkers to diagnose these chronic maladies, to predict inflammation and disease progression, as well as monitor the effectiveness of treatments. Further, we discuss the urgency for fast and reliable point-of-care methods to detect and quantify crucial protein biomarkers in sputum specimens, and limitations faced when dealing with their complex matrices.

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Section: Advanced Technologies To Enhance Lateral Flow Assay Analytic...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

Preprint

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“…This blocking mechanism was activated electrokinetically to drive the delivery of the label once the antigen-antibody is in the test zone. The limit of detection using electrophoretically driven LFIA (eLFIA), has been proven to be 1000 times lower than that of conventional LFIA [63]. Various post-assay upgrades based on chemical reactions to facilitate high sensitivity for LFIA were presented in a critical review [64].…”

Section: Advanced Technologies To Enhance Lateral Flow Assay Analytic...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

IJTM

View full text Add to dashboard Cite

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage of formation, these disorders can be effectively treated, leading to successful outcomes; curative therapies can halt illnesses from advancing, thus improving the quality of life and long-term survival of the patient. Still, cutting-edge upgrades in precision technologies are necessary for early, reliable, affordable, and rapid disease detection, but also vital for the well-being of people and the future of global public health. The emerging role and utility of non-invasive and repeatable diagnostic test approaches for the detection of health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator or signature (e.g., proteins) to provide valuable information on an individual’s wellness and/or disease. Among the bodily secretions used for non-invasive diagnostics is sputum, a complex viscous gel-like biopolymeric network that has gained growing recognition as a rich source of biomarkers of airway infections and pulmonary diseases, and serves as a determinant to reveal other illnesses. As per the World Health Organization, the burden of respiratory conditions is exacerbated by factors ranging from considerable subjection to air pollution and occupational contaminants to tobacco smoking and second-hand smoke, in addition to poor socio-economic status. Due to the likely increase in these determinants, respiratory tract ailments are on the rise, affecting the health of many individuals, in addition to putting stress on healthcare facilities and services worldwide. The aim of this study was to perform a narrative review of sputum constituents with an emphasis on proteins and glycoproteins assessed as possible biomarkers of lung and other organ diseases. A search was conducted using mucus, sputum proteomics, sputum biomarkers, and point-of-care testing as keywords employing Google, PubMed (MEDLINE), and Web of Science, selecting the most referenced and related papers of the last decade. We, therefore, highlight the need to use expectorated or induced sputum specimens as a routine sample source for testing valuable protein biomarkers to diagnose these chronic disorders, predict inflammation and disease progression, as well as monitor the effectiveness of treatments. Further, we discuss the urgent need for fast and reliable point-of-care methods to detect and quantify crucial protein biomarkers in sputum specimens, and the limitations faced when dealing with their complex matrices.

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“…15 showcases several examples of colorimetric, electrochemical, and fluorescence detection methods. The combination of colorimetric and electrochemical techniques 170 has demonstrated a 10 3 -fold lower LoD than ordinary lateral flow assays, resulting in successful clinical serological implementation. This improvement in the LoD can be attributed to the electrostacking of gold nanoparticle-labeled proteins.…”

Section: Combination Of Lfa Strips With Portable Optical Readers For ...mentioning

confidence: 99%

Recent advances in point-of-care testing of COVID-19

Lee,

Bi,

Chen

et al. 2023

Chem. Soc. Rev.

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Electrophoretic Assembly of Antibody–Antigen Complexes Facilitates 1000 Times Improvement in the Limit of Detection of Serological Paper-Based Assay

Cited by 8 publications

References 48 publications

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Recent advances in point-of-care testing of COVID-19

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