Electrophoresis of NADH-dependent oxidoreductase (diaphorase) in boar spermatozoa: Elektrophorese der NADH-abhängigen Oxydoreduktase (Diaphorase) in Eberspermatozoen

Abstract

Two forms of NADH-dependent oxidoreductase (diaphorase rEC.1.6.99.-1) are established in boar spermatozoa. The first form is typical for soluble proteins with a varying electrophoretic profile, while the other form for sedimental proteins with a specific, slowlymoving fraction, which is not common for the soluble form. The two enzyme forms have a close isoelectric point (PI 5.5-6.0) and they can not be inhibited by dicumarol moll-' and FAD moll-'. The molecular mass of the soluble form of the enzyme is 28,37,46 and 67 kD, while of the sedimental form it is 220, 250 and 260 kD, respectively.Zusammenfassung. In Eberspermatozoen sind zwei Formen der NADH-abhangigen Oxydoreduktase (Diaphorase rEC.1.6.99.-1) vorhanden. Die erste Form ist typisch fur losliche Proteine mit einem unterschiedlichen elektrophoretischen Profil, die andere Form fur Sediment-Proteine mit einer spezifischen, langsam wandernden Fraktion, die nicht ublich ist fur die losliche Form. Die beiden Enzyme haben einen nahe beieinander liegenden isoelektrischen Punkt (PI 5,s-6,O); sie konnen nicht durch Dicumarol mol-' und FAD mol-' gehemmt werden. Das Molekulargewicht der loslichen Form des Enzyms ist 28, 37,46 und 67 kD, wahrend dieses fur die Sediment-Form 220,250 und 260 kD betragt. In memoriam:Professor Tapani Vanha-Perttula, Professor and Chairman of Anatomy, University of Kuopio, and the former Rector of the University of Kuopio, was killed in a traffic accident with an elk on February 11, 1990, in Kuopio.Tapani Vanha-Perttula was very successful in his research activities. He published more than 140 original full length papers. He also investigated the presence of trace elements in the seminal plasma and in the sperms and the effect of these elements on fertilization as well as the effects of enzyme inhibitors on the maturation of sperms and their fertilizing ability.The Editors of andrologia lost a very good friend; he published many excellent papers and served as a referee for our journal.androloPia 22. No. 6 (1990)