Electrophoresis of human haemoglobins in starch gel

Multiple Haemoglobins

“…A high-voltage modification of this technique has been applied to haemoglobins by Kunzer & Ambs In recent years particularly good resolution of components has been obtained by the use of gels as supporting media. Starch gel (Smithies, 1955) has been used for human haemoglobins by a number of workers (Owen & Got, 1957;Goldberg, 1958;de Grouchy, 1958). Agar gel has also been used for analysis of animal haemoglobins by Fine, Uriel & Faure (1956), Giri & Pillai (1956) and Monnier & Fischer (1958) all using a pH near 8.6.…”

A Critical Review of Human Haemoglobin Variants

“…The solubility curves of Hb-F have been examined by Derrien and co-workers with the results already mentioned, by Giraud, Orsini, and Le Poullain (1956), Polosa et al (1957c, 1957d), and Pagliardi et al (1954, all using the salting-out procedure, and by Itano (1953a), White and Beaven (1954), , and Wyman, Rafferty, and Ingalls (1944). Laurent. Bouscayrol, Dunan, and Borgomano (1956) have carried out salting-out experiments using adult, foetal, and thalassaemic methaemoglobins, and observe differences in the shapes of the curves compared with the corresponding carboxyhaemoglobins.…”

“…), and it is also clearly demarcated in agar Giri and Pillai, 1956), starch slab , starch gel (Owen and Got, 1957;de Grouchy, 1958), and by isoelectric line spectra (Tuttle, 1956). Its behaviour in free boundary electrophoresis is also distinctive (Itano and Neel, 1950;Wells and Itano, 1951 ;Sturgeon et a1., 1952;Zinsser, 1952;Singer et al, 1954;Shooter and Skinner, 1955), and indeed the first fractionation was achieved by this method (Pauling et al, 1949).…”

“…Good separation has been achieved on starch gel by Owen and Got (1957), de Grouchy (1958), and de Grouchy et al (1958 and on agar by Robinson et al (1957), as well as by an isoelectric line spectra method by Tuttle (1956). A particularly striking separation on starch block at high voltage has been reported by Kunzer and Ambs (1958).…”

A Further Abnormality of Foetal Haemoglobin

“…Starch-gel electrophoresis has been first used successfully by Smithies for the separation of serum proteins (1); subsequently other investigators have used this supporting medium for electro phoresis of haemoglobins (2)(3)(4)(5). In this laboratory starch-gel was found to be definitely superior to paper electrophoresis in demon strating clearly small haemoglobin fractions (6).…”