Electrophoresis of Human Gastric Juice

The electrophoresis of human gastric juice: Part I In normal controls

“…Henning usually found four bands on electrophoresis, the fast moving fraction corresponding to human albumin. A major advance was made when Gullberg and Olhagen (1959) studied the electrophoretic pattern of gastric juice, autodigestion of which had been prevented by intragastric neutralization with phosphate buffer. They found three main proteinstaining components.…”

Gamma Globulin Turnover and Intestinal Degradation of Gamma Globulin in the Dog*

“…Albumin but no gamma globulin has been found by electrophoresis of gastric content (35,36), whereas substantial amounts of gamma globulin have been found in intestinal juice (6). Glenert, Jarnum, and Riemer found that gastric loss was responsible for only about 10% of albumin catabolism, and therefore it is unlikely that gastric loss of gamma globulin should be that great.…”

Protein content of gastric juice: Part II In normal subjects and patients suffering from peptic ulcer and gastric carcinoma

“…Its presence was subsequently fully confirmed using electrophoretic and immunological techniques (Heiskell et al, 1961;Glass and Ishimori, 1961;Gullberg and Olhagen, 1959;Piper et al, 1962). Immunological and electrophoretic techniques can be partially invalidated by intragastric autodigestion (Piper et al, 1962) and intragastric neutralization to prevent autodigestion makes quantitative studies of protein concentrations and output impossible.…”