Electrooxidation and Kinetic Study of p-Nitroaniline in Acidic Solution

Paliwal, Richa Paliwal

doi:10.22214/ijraset.2018.2036

Cited by 2 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pNA displayed an oxidation peak at 1.1 V vs Ag/AgCl and a reduction peak at −0.80 vs Ag/AgCl. This oxidation peak represents oxidation of the amino group of pNA, 18 whereas the reduction peak of pNA represents a reduction in the nitro group of pNA. 19 The reduction current of pNA is larger than the oxidation current of the pNA because of the difference in the number of transferred electrons.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Highly Sensitive Electrochemical Immunoassay Using Signal Amplification of the Coagulation Cascade

et al. 2022

View full text Add to dashboard Cite

Here, we report a highly sensitive immunoassay for human immunoglobulin G (IgG) that uses signal amplification of the coagulation cascade. Z-Phe-Pro-Lys-p-nitroaniline (FPK-pNA) was used as a substrate for thrombin activation in the last step of the coagulation cascade. During the coagulation cascade, pNA is liberated from FPK-pNA and can be detected electrochemically. Using square wave voltammetry with a glassy carbon electrode, we demonstrated that pNA can be quantified in a solution modeling the coagulation cascade prepared by mixing FPK-pNA and pNA. Characterization of the reactivity of thrombin toward FPK-pNA revealed that thrombin efficiently reacted with FPK-pNA. Subsequent characterization of factor XIa activity of factor XIalabeled antibody revealed that factor XIa was not inactivated during labeling. Finally, a coagulation cascade-based immunoassay for human IgG was performed using a factor XIa-labeled antibody on magnetic beads. The limit of detection for human IgG was 5.0 pg/mL (33 fM) indicating that the coagulation cascade can amplify the immunoassay sensitivity compared to immunoassay using a thrombin-labeled antibody as a condition without a coagulation cascade. Coagulation cascade-based immunoassay was also highly selective. In the near future, we will report a highly sensitive immunoassay for the simultaneous detection of multiple analytes using a coagulation cascade-based immunoassay and Limulus amebocyte lysate reaction-based immunoassay we previously reported.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%