Highly Sensitive Electrochemical Immunoassay Using Signal Amplification of the Coagulation Cascade

Ito, Kinuko; Inoue, Kumi Y.; Ino, Kosuke; Shiku, Hitoshi

doi:10.1021/acs.analchem.2c02241

Cited by 6 publications

(15 citation statements)

References 30 publications

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“…The calibration plots for goat and human IgG were not linear because of using cascade reactions. In our previous study using LAL or coagulation cascade reaction, ,, the same trend of nonlinear calibration plots was obtained. Performing curve fitting of calibration plots will be realized for practical use in the future.…”

Section: Resultssupporting

confidence: 66%

“…Based on thrice the standard deviation of 0 pg/mL human IgG (BG + 3σ), the limit of detection for human IgG with dual-signal amplification is 50 fg/mL (330 amol/L). This result shows that the limit of detection is improved by 100-fold compared to our previous study in which an immunoassay was performed with single-signal amplification of the coagulation cascade reaction . This improvement is mainly attributed to the signal amplification of redox cycling.…”

Section: Resultsmentioning

confidence: 56%

“…However, to our best knowledge, a multiplex immunoassay with signal amplification of a cascade reaction has not been reported. A multiplex immunoassay has many advantages such as cost and time reduction, particularly for diagnosis requiring detection of multiple marker molecules. , In this study, we developed a dual immunoassay with signal amplification of a cascade reaction and redox cycling within a nanoscale gap, based on our previous studies on immunoassays using LAL and coagulation cascade reactions . Endotoxin and factor XIa were employed as the label molecules for the immunoassays of goat and human IgG, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…The other immunoassay was based on signal amplification of a coagulation cascade reaction. 10 We performed immunoassays for human IgG using the activated form of factor XI (factor XIa), which induces a blood coagulation cascade reaction, as the label molecule. After the antigen−antibody reaction, the coagulation cascade reaction was induced, allowing detection of p-nitroaniline (pNA) liberated from peptide-conjugated pNA (Z−Phe−Pro−Lys− pNA; FPK−pNA) at its end.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The liberated p AP was detected at the nanoscale-gap electrode, amplifying the amperometric signals by repeating the redox reaction between two close electrodes, and a detection limit of 470 amol/L was achieved for goat IgG. The other immunoassay was based on signal amplification of a coagulation cascade reaction . We performed immunoassays for human IgG using the activated form of factor XI (factor XIa), which induces a blood coagulation cascade reaction, as the label molecule.…”

Section: Introductionmentioning

confidence: 99%

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High-Sensitivity Amperometric Dual Immunoassay Using Two Cascade Reactions with Signal Amplification of Redox Cycling in Nanoscale Gap

et al. 2022

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Here, we report a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode. Endotoxin and factor XIa were used as the label molecules for the immunoassay of two types of analytes to induce the LAL and coagulation cascade reactions, respectively, when each corresponding analyte existed in the sample solution. In addition to the signal amplification by the cascade reactions, we employed redox cycling in a nanoscale gap to achieve a highly sensitive assay. The nanoscale-gap electrode amplifies the amperometric signals from paminophenol liberated from artificial substrates in the final steps of the cascade reactions. First, the cross reaction between the LAL and coagulation cascade reactions was investigated. The results indicated that these cascade reactions did not efficiently proceed in a single solution owing to the cross reaction. Therefore, we selected to induce two cascade reactions in different solutions by bisecting the beads after the immunocomplex formation on the beads. The cross reactions of factor XIa with the LAL cascade reaction and of endotoxin with the coagulation cascade reaction were investigated. The effects of these cross reactions were revealed to be negligible by bisecting the beads before inducing the cascade reactions. Finally, a dual immunoassay for goat and human immunoglobulin G was performed, for which the limits of detection were 70 pg/mL (470 fmol/L) and 1.0 ng/mL (6.6 pmol/L), respectively. Thus, our dual immunoassay provides a sensitive platform for clinical diagnosis requiring detection of multiple analytes.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-Sensitivity Amperometric Dual Immunoassay Using Two Cascade Reactions with Signal Amplification of Redox Cycling in Nanoscale Gap

et al. 2022

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Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis

Ji,

Wang,

Zhao

2024

J. Agric. Food Chem.

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Marine biotoxins are metabolites produced by algae that can accumulate in shellfish or fish and enter organisms through the food chain, posing a serious threat to biological health. Therefore, accurate and rapid detection is an urgent requirement for food safety. Although various detection methods, including the mouse bioassay, liquid chromatography−mass spectrometry, and cell detection methods, and protein phosphatase inhibition assays have been developed in the past decades, the current detection methods cannot fully meet these demands. Among these methods, the outstanding immunoassay virtues of high sensitivity, reliability, and low cost are highly advantageous for marine biotoxin detection in complex samples. In this work, we review the recent 5-year progress in marine biotoxin immunodetection technologies such as optical immunoassays, electrochemical immunoassays, and piezoelectric immunoassays. With the assistance of immunoassays, the detection of food-related marine biotoxins can be implemented for ensuring public health and preventing food poisoning. In addition, the immunodetection technique platforms including lateral flow chips and microfluidic chips are also discussed. We carefully investigate the advantages and disadvantages for each immunoassay, which are compared to demonstrate the guidance for selecting appropriate immunoassays and platforms for the detection of marine biotoxins. It is expected that this review will provide insights for the further development of immunoassays and promote the rapid progress and successful translation of advanced immunoassays with food safety detection.

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Mixing performance of an expansive mixer on viscoelastic solutions under alternating current electric field

Wang,

Xu,

2023

Physics of Fluids

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Development of effective technologies for mixing viscoelastic solutions with reagents is still a challenge, which plays an important role in the fields of disease diagnosis, water desalination, and immunoassay. The purpose of this paper is to numerically study the mixing performance of an expansive mixer on the viscoelastic solution under the impact of an alternating current (AC) electric field through the analyzation of mixing efficiency, the concentration distribution, and average outlet velocity. A poly acrylic acid (PAA) water solution with different concentrations characterized by the Oldroyd-B constitutive model is used as a mixed medium, and an open-source toolbox called rheoTool based on finite volume algorithm is applied to solve the governing equations. We quantitatively determine the mixing efficiency for different PAA concentrations, AC electric field strengths, and frequencies and compare the mixing efficiency created by AC and direct current (DC) electric fields. Our investigation indicates that the mixing efficiency of the mixer diminishes with the PAA concentrations but enhances with the AC electric field strengths for a relatively high polymer concentration, and AC electric fields with different frequencies lead to a larger mixing efficiency than DC electric fields for a low polymer concentration. Additionally, the influence of the model parameters on the average outlet velocity and concentration distribution is discussed at length. Our numerical simulation results show that the mixer has an excellent mixing performance for both Newtonian and viscoelastic solutions, with a mixing efficiency of more than 99% at a low polymer concentration and a stable outflow velocity.

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Highly Sensitive Electrochemical Immunoassay Using Signal Amplification of the Coagulation Cascade

Cited by 6 publications

References 30 publications

High-Sensitivity Amperometric Dual Immunoassay Using Two Cascade Reactions with Signal Amplification of Redox Cycling in Nanoscale Gap

High-Sensitivity Amperometric Dual Immunoassay Using Two Cascade Reactions with Signal Amplification of Redox Cycling in Nanoscale Gap

Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis

Mixing performance of an expansive mixer on viscoelastic solutions under alternating current electric field

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