Electroosmotic flow controllable coating on a capillary surface by a sol–gel process for capillary electrophoresis

Hsieh, Yi-Yun; Lin, Ya-Hsien; Yang, Jr-Shiung; Wei, Guor‐Tzo; Tien, Pei; Chau, Lai-Kwan

doi:10.1016/s0021-9673(02)00079-1

Cited by 22 publications

(8 citation statements)

References 42 publications

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“…The ability to control the magnitude of the EOF is important in optimizing separations [21,27,[29][30][31][33][34][35]43]. The EOF of the DODAB/POE stearate coating ( eof,DODAB + POE stearate ) is suppressed as the length of the POE block increases (Table 1).…”

Section: Adjustable Eof With Dodab/poe Stearate Coatingsmentioning

confidence: 99%

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A modified supported bilayer/diblock polymer – Working towards a tunable coating for capillary electrophoresis

MacDonald¹,

Bahnasy²,

Lucy³

2011

Journal of Chromatography A

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Section: Adjustable Eof With Dodab/poe Stearate Coatingsmentioning

confidence: 99%

“…With many tunable coatings the adjustable EOF is in response to changes in the buffer pH [27][28][29][30]. However, pH is an important variable in the optimization of a protein separation.…”

Section: Introductionmentioning

confidence: 99%

A modified supported bilayer/diblock polymer – Working towards a tunable coating for capillary electrophoresis

MacDonald¹,

Bahnasy²,

Lucy³

2011

Journal of Chromatography A

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“…When the methanol content changed to 0%, only four peaks are observed, and the resolution is the worst. The peak shapes of basic compounds were better than reported , which is attributed to the repulsion of the positively charged amine groups on the stationary phase of the positively charged basic compounds and thus prevent them from approaching the residual silanol groups. In this part, the separation process is caused by the octyl RP mechanism and the electrophoretic migration.…”

Section: Resultsmentioning

confidence: 61%

Open tubular columns with mixed‐mode reversed‐phase and weak anion‐exchange stationary phase for capillary electrochromatography

Lei

Zhang

2013

J of Separation Science

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Columns for open tubular capillary electrochromatography, coated with a mixed-mode (RP/ion-exchange) stationary phase, were prepared by using the sol-gel method. The synthetic procedure was optimized by changing the ratios of tetraethoxysilane, octyltriethoxysilane, and 3-aminopropyltriethoxysilane in the initial sol. SEM studies reveal that a coating with about 400 nm thickness can be obtained. The inner surface properties of these capillaries were probed by measuring the EOF as a function of pH. The surface of this stationary phase contains octyl, amine, and residual silanol moieties; the amine and silanol groups determine the net charge on the inner surface of the capillary and can produce a switchable EOF (anodal/cathodal). The performances of the columns were evaluated by open tubular capillary electrochromatography using a wide range of compounds (polycyclic aromatic hydrocarbons, aromatic acids, and aromatic amines).

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“…There are several strategies that have been utilized for immobilizing biomolecules in MOFs, including encapsulation, covalent bonding, and physical adsorption . Among these methods, physical adsorption, without any chemical modification, is the best way to immobilize enzyme .…”

Section: Introductionmentioning

confidence: 99%

A combined theoretical and experimental study to improve the thermal stability of recombinant D‐lactate dehydrogenase immobilized on a novel superparamagnetic Fe₃O₄NPs@metal–organic framework

Torkzadeh‐Mahani

Zaboli

Barani

2020

Applied Organom Chemis

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Lactate dehydrogenase (LDH) is an enzyme that catalyzes the reduction of nicotinamide adenine dinucleotide (NADH) and pyruvate to nicotinamide adenine dinucleotide (NAD+) and D‐lactate in the final step of anaerobic glycolysis. This enzyme belongs to the family of oxidoreductases. Humans possess two isoforms of LDH enzyme: NAD‐dependent L‐lactate dehydrogenase (L‐LDH) and NAD‐dependent D‐lactate dehydrogenase (D‐LDH). D‐LDH is released during tissue damage, and is a sign of diseases such as kidney stones, heart failure, and some types of cancers and appendicitis. Accordingly, the design and construction of biosensors for the determination of lactate levels are important. The thermal sensitivity of D‐LDH and low protein production in the host bacteria limit the use of this protein in certain applications. To solve these problems, two solutions were used in this study. First, the codon‐optimized 1008 bp D‐LDH gene fused with a histidine tag was cloned at the NcoI/XhoI sites and expressed in E. coli BL21. Second, a new metal–organic framework (Fe3O4NPs@Ni‐MOF) was synthesized and used for immobilization and stabilization of D‐LDH. Fe3O4NPs@Ni‐MOF core‐shell nanocomposites were characterized by Fourier transform infrared spectroscopy, vibrating sample magnetometer, scanning electron microscopy, X‐ray diffraction, and the Brunauer–Emmett–Teller method. In comparison with the free enzyme, the immobilized enzyme presented better stability at high temperatures. The immobilization of the enzyme could be useful because most reactions happen at high temperatures in industry. To examine the effect of Fe3O4NPs@Ni‐MOF on the adsorption and conformation of D‐LDH at the atomistic level, a molecular dynamics simulation was carried out. Our study showed that the interaction between Fe3O4NPs@Ni‐MOF and D‐LDH involved van der Waals interactions, hydrophobic interaction energies, cation–π interaction between the Ni ions of the MOF with the enzyme residues and also, the hydrogen bond interactions between enzyme and heteroatoms in the MOF. Root mean square fluctuation and secondary structure analysis showed that Fe3O4NPs@Ni‐MOF protected the conformation of the enzyme.

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Electroosmotic flow controllable coating on a capillary surface by a sol–gel process for capillary electrophoresis

Cited by 22 publications

References 42 publications

A modified supported bilayer/diblock polymer – Working towards a tunable coating for capillary electrophoresis

A modified supported bilayer/diblock polymer – Working towards a tunable coating for capillary electrophoresis

Open tubular columns with mixed‐mode reversed‐phase and weak anion‐exchange stationary phase for capillary electrochromatography

A combined theoretical and experimental study to improve the thermal stability of recombinant D‐lactate dehydrogenase immobilized on a novel superparamagnetic Fe₃O₄NPs@metal–organic framework

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Electroosmotic flow controllable coating on a capillary surface by a sol–gel process for capillary electrophoresis

Cited by 22 publications

References 42 publications

A modified supported bilayer/diblock polymer – Working towards a tunable coating for capillary electrophoresis

A modified supported bilayer/diblock polymer – Working towards a tunable coating for capillary electrophoresis

Open tubular columns with mixed‐mode reversed‐phase and weak anion‐exchange stationary phase for capillary electrochromatography

A combined theoretical and experimental study to improve the thermal stability of recombinant D‐lactate dehydrogenase immobilized on a novel superparamagnetic Fe3O4NPs@metal–organic framework

Contact Info

Product

Resources

About

A combined theoretical and experimental study to improve the thermal stability of recombinant D‐lactate dehydrogenase immobilized on a novel superparamagnetic Fe₃O₄NPs@metal–organic framework