Electronic sorting and recovery of single live cells from microlitre sized samples

Fuchs, Alexandra; Romani, Annalisa; Freida, Delphine; Medoro, Gianni; Abonnenc, Mélanie; Altomare, Luigi; Chartier, Isabelle; Guergour, Dorra; Villiers, Christian; Marche, Patrice N.; Tartagni, Marco; Guerrieri, R.; Chatelain, François; Manaresi, Nicolò

doi:10.1039/b505884h

Cited by 96 publications

(70 citation statements)

References 18 publications

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“…In order to have a dynamic single-cell control, an active DEP device is required. This has been demonstrated by the creation of dynamic DEP traps on a CMOS-circuit-based device [21]. However, the current incarnation of the device limits the resolution to 20-µm-diameter cells or larger, if single-cell trapping is desired.…”

Section: Introductionmentioning

confidence: 99%

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Ohta

Chiou

Phan³

et al. 2007

IEEE J. Select. Topics Quantum Electron.

121

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Abstract-Optoelectronic tweezers (OET) provide a powerful tool for the manipulation of micro-and nanoparticles. The OET device produces an optically controlled dielectrophoretic force, allowing complex dynamic manipulation patterns using light intensities up to 100 000 times lower than that of optical tweezers. Using OET, we demonstrate the separation of live and dead human B cells, and the separation of HeLa and Jurkat cells. We also present, for the first time, a modified single-sided OET device that promises to facilitate the integration of OET and microfluidics. Unlike standard OET, this single-sided OET device produces electric fields that are oriented parallel to the plane of the device. We demonstrate the manipulation of polystyrene beads using this new single-sided OET device, and discuss its capabilities. Index Terms-Dielectrophoresis (DEP), optical tweezers, optically induced dielectrophoresis, optoelectronic tweezers (OET).

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Section: Introductionmentioning

confidence: 99%

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Ohta

Chiou

Phan³

et al. 2007

IEEE J. Select. Topics Quantum Electron.

121

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“…Microfluidic systems can be used to demonstrate the feasibility of integration of the multiple steps involved in single-cell analysis, 1-10 such as separating, positioning, stimulating, and detecting, which are impossible with other widely used single-cell analysis methods such as automated microscopy, 11 flow cytometry, 12 and laser scanning cytometry. 13 Numerous microfluidic-based single-cell analysis methods have been developed and can be classified according to the positioning methods, such as electrical positioning, 14 hydrodynamic trapping, 15 physical trapping, 16,17 and microwell trapping. 18,19 However, these methods are inappropriate for the detection of environmental factors such as cell-cell contact and cell-extracellular matrix ͑ECM͒ contact.…”

Section: Introductionmentioning

confidence: 99%

Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system

Jang

Tanaka

et al. 2010

Biomicrofluidics

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Recently, interest in single cell analysis has increased because of its potential for improving our understanding of cellular processes. Single cell operation and attachment is indispensable to realize this task. In this paper, we employed a simple and direct method for single-cell attachment and culture in a closed microchannel. The microchannel surface was modified by applying a nonbiofouling polymer, 2-methacryloyloxyethyl phosphorylcholine ͑MPC͒ polymer, and a nitrobenzyl photocleavable linker. Using ultraviolet ͑UV͒ light irradiation, the MPC polymer was selectively removed by a photochemical reaction that adjusted the cell adherence inside the microchannel. To obtain the desired single endothelial cell patterning in the microchannel, cell-adhesive regions were controlled by use of round photomasks with diameters of 10, 20, 30, or 50 m. Single-cell adherence patterns were formed after 12 h of incubation, only when 20 and 30 m photomasks were used, and the proportions of adherent and nonadherent cells among the entire UVilluminated areas were 21.3% Ϯ 0.3% and 7.9% Ϯ 0.3%, respectively. The frequency of single-cell adherence in the case of the 20 m photomask was 2.7 times greater than that in the case of the 30 m photomask. We found that the 20 m photomask was optimal for the formation of single-cell adherence patterns in the microchannel. This technique can be a powerful tool for analyzing environmental factors like cell-surface and cell-extracellular matrix contact.

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“…Electrode-based DEP devices have parallel manipulation capabilities, but the trapping patterns are fixed, and it is difficult to isolate a single particle of interest. This capability can be achieved by creating dynamic DEP cages via CMOS control (Fuchs et al, 2006). However, this device is limited by the pitch of the CMOS circuitry, and is currently limited to microscale manipulation.…”

Section: Dielectrophoresismentioning

confidence: 99%

Optoelectronic Tweezers for the Manipulation of Cells, Microparticles, and Nanoparticles

Ohta¹,

Chiou²,

Jamshidi³

et al. 2010

Recent Optical and Photonic Technologies

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Electronic sorting and recovery of single live cells from microlitre sized samples

Cited by 96 publications

References 18 publications

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system

Optoelectronic Tweezers for the Manipulation of Cells, Microparticles, and Nanoparticles

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