Electronic profiling of membrane antigen expressionviaimmunomagnetic cell manipulation

Abstract

A disposable magnetophoretic cytometer with a multiplexed electronic readout for quantitative membrane antigen profiling.

Novel Approaches Concerning the Numerical Modeling of Particle and Cell Separation in Microchannels: A Review

“…The simulations were used to predict the trajectory and capturing region of the cells and to analyze the effect of the magnetic bead size, cell size, number of beads per cell, and flow rate on the separation performance. On the other hand, Civelekoglu et al [36], with the aid of numerical simulations, introduced a cytometry technique that computed the surface expression of immunomagnet-ically labeled cells by electrically tracking their trajectory under a magnetic field gradient on a microfluidic chip with a throughput of >500 cells/min. More specifically, immunomagnetically labeled cells were deflected under a magnetic field gradient according to their magnetic load (or surface expression) and detected at the different outlets of the device (Figure 5).…”

“…Figure 5. Device designed by Civelekoglu et al[36] for cell sorting based on the expression of surface molecules. Immunomagnetically labeled cells are introduced to the microfluidic device from a single inlet.…”

Immunomagnetic leukocyte differential in whole blood on an electronic microdevice

“…We recently developed an integrated microfluidic chip that can profile cell surface antigens directly from whole blood samples using immunomagnetic sorting 36,37 as a point-ofcare-suitable form of flow cytometry. [38][39][40] In this work, we apply our technology to develop an electronic leukocyte differential assay that can directly analyze whole blood samples on a low-cost and portable microfluidic chip.…”

Microscale magnetic field modulation using rapidly patterned soft magnetic microstructures

“…2 Magnetic objects include not only intrinsically magnetic samples such as magnetotactic bacteria, 3 but also samples that are labelled with magnetic particles. 4 The use of magnetic particles specically conjugated to targets offer great selectivity allowing various applications for separation or detection, including immunomagnetic separation of cells, 5 proteins, 6 and nucleic acids, 7 the detection of pathogens. 7 Besides, with the aid of controllable magnetic forces by designing magnetic elds, cells can guide toward designed spatial arrangements, 8,9 which make it suitable for the organization of cells such as to align and migrate cells into a targeted pattern 10 and to pattern cells at specic points.…”