Electronic DNA hybridisation detection in low-ionic strength solutions

Braeken, Dries; Reekmans, Gunter; Zhou, Cheng; Meerbergen, Bart Van; Callewaert, Geert; Borghs, Gustaaf; Bartic, Carmen

doi:10.1080/17458080802163421

Cited by 10 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Direct electrostatic immobilization of DNA molecules onto the FED surface is, in general, impossible due to electrostatic repulsion forces between the DNA and the FED surface with typically negatively charged gate insulators (e.g., SiO 2 , Ta 2 O 5 , Si 3 N 4 ). Therefore, a modification of the sensor surface by means of layer-by-layer (LbL) electrostatic adsorption of a cationic polyelectrolyte/ssDNA bilayer and subsequent hybridization with cDNA molecules becomes more popular in FED-based DNA biosensors design. ,,− In contrast to often applied covalent immobilization methods that require time-consuming, cost-intensive procedures and complicated chemistry for functionalization of the gate surface and/or probe ssDNA, the LbL electrostatic adsorption technique is easy, fast, and applicable for substrates with any shapes and form. ,, The suitability of FEDs for the detection of adsorptively immobilized DNA has been demonstrated by modifying the gate surface of an EIS sensor and a floating-gate field-effect transistor by the positively charged poly- l -lysine. However, the recorded DNA-immobilization and hybridization signals were small (several mVs).…”

Section: Introductionmentioning

confidence: 99%

DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer

Bronder

Poghossian

Scheja

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance-voltage and constant-capacitance measurements. In addition, the surface morphology of the PAH layer was studied by atomic force microscopy and contact-angle measurements. High hybridization signals of 34 and 43 mV were recorded in low-ionic strength solutions of 10 and 1 mM, respectively. In contrast, a small signal of 4 mV was recorded in the case of unspecific adsorption of fully mismatched ncDNA. The density of probe ssDNA and dsDNA molecules as well as the hybridization efficiency was estimated using the experimentally measured DNA immobilization and hybridization signals and a simplified double-layer capacitor model. The results of field-effect experiments were supported by fluorescence measurements, verifying the DNA-immobilization and hybridization event.

show abstract

Section: Introductionmentioning

confidence: 99%

DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer

Bronder

Poghossian

Scheja

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…In this context, non-covalent surface attachment of probe ssDNA using electrostatic and/or hydrophobic interactions becomes more and more popular in DNA sensor design, including FED-based DNA biosensors. 38,[45][46][47] In this work, we present a new MLAPS-based DNA biosensor, where a simple strategy is used for a rapid immobilization of probe ssDNA molecules onto the gate surface via the layerby-layer (LbL) electrostatic adsorption of a weak-polyelectrolyte/ssDNA bilayer, and the subsequent label-free detection of DNA hybridization. If probe ssDNA molecules preferentially lie flat on the full length of the LAPS surface with negatively charged phosphate groups directed to the positively charged polyelectrolyte layer (in this study, poly(allylamine hydrochloride) (PAH)) and the bases are exposed to the surrounding solution and readied for hybridization with complementary target DNA molecules, both the Debye screening effect and the electrostatic repulsion between target and probe DNA molecules are less effective, and therefore, a higher hybridization signal can be expected.…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of DNA using a light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer

Bronder

Poghossian

et al. 2015

Nanoscale

View full text Add to dashboard Cite

A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent-voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.

show abstract

“…As represented in Fig. 5c, d, the analytes captured beyond the Debye length do not influence the Gao et al (2012); Hahm and Lieber 2004;Li et al (2004); Lin et al (2009); Zheng et al (2010) NW arrays 1E−13 to 1E−16 50 to 3600 Lu et al (2014); Zhang et al (2015) Planar FETs 1E−4 to 1E−7 300 to 54,000 1E−5 to 1E−7 300 to 1200 Braeken et al (2008); Freeman et al (2007); Sakata et al (2005); Shin et al 2004); Uno et al (2007); Xu et al (2016); Zafar et al (2018); Zayats et al (2006) Fig . 3 a-d Schematics of the iso-concentration diffusion lines (in blue) in close proximity and further from the sensor surface on planar, silicon nanowire, nanowires array, and high aspect ratio Fin FET, respectively.…”

Section: Limitations Of Bio-sensing Using Fetsmentioning

confidence: 93%

The influence of geometry and other fundamental challenges for bio-sensing with field effect transistors

et al. 2019

View full text Add to dashboard Cite

We present a review of field effect transistors (FET) from the point of view of their applications to label-free sensing in the era of genomics and proteomics. Here, rather than a collection of Bio-FET achievements, we propose an analysis of the different issues hampering the use of these devices into clinical applications. We make a particular emphasis on the influence of the sensor geometry in the phenomena of mass transport of analytes, which is a topic that has been traditionally overlooked in the analysis and design of biosensors, but that plays a central role in the achievement of low limits of detection. Other issues like the screening of charges by the ions in liquids with physiological ionic strength and the non-specific binding are also reviewed. In conclusion, we give an overview of different solutions that have been proposed to address all these challenges, demonstrating the potential of field effect transistors owing to their ease of integration with other semiconductor components for developing cost-effective, highly multiplexed sensors for next-generation medicines.

show abstract

Electronic DNA hybridisation detection in low-ionic strength solutions

Cited by 10 publications

References 14 publications

DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer

DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer

Label-free detection of DNA using a light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer

The influence of geometry and other fundamental challenges for bio-sensing with field effect transistors

Contact Info

Product

Resources

About