Electronic Circular Dichroism of Peptide Nucleic Acids and their Analogues

Corradini, Roberto; Tedeschi, Tullia; Sforza, Stefano; Marchelli, Rosangela

doi:10.1002/9781118120392.ch18

Cited by 12 publications

(27 citation statements)

References 93 publications

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“…PNA are achiral molecules, but chiral centers can be introduced by adding amino acids into the PNA oligomer or at its terminus (typically a lysine is added). As a result, CD can be observed confirming the helicity of PNA-PNA, PNA-DNA, and PNA-RNA duplexes [56].…”

Section: Pna Complexes With Natural Nucleic Acidsmentioning

confidence: 57%

Antibacterial Peptide Nucleic Acids—Facts and Perspectives

et al. 2020

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Antibiotic resistance is an escalating, worldwide problem. Due to excessive use of antibiotics, multidrug-resistant bacteria have become a serious threat and a major global healthcare problem of the 21st century. This fact creates an urgent need for new and effective antimicrobials. The common strategies for antibiotic discovery are based on either modifying existing antibiotics or screening compound libraries, but these strategies have not been successful in recent decades. An alternative approach could be to use gene-specific oligonucleotides, such as peptide nucleic acid (PNA) oligomers, that can specifically target any single pathogen. This approach broadens the range of potential targets to any gene with a known sequence in any bacterium, and could significantly reduce the time required to discover new antimicrobials or their redesign, if resistance arises. We review the potential of PNA as an antibacterial molecule. First, we describe the physicochemical properties of PNA and modifications of the PNA backbone and nucleobases. Second, we review the carriers used to transport PNA to bacterial cells. Furthermore, we discuss the PNA targets in antibacterial studies focusing on antisense PNA targeting bacterial mRNA and rRNA.

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Section: Pna Complexes With Natural Nucleic Acidsmentioning

confidence: 57%

Antibacterial Peptide Nucleic Acids—Facts and Perspectives

et al. 2020

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“…10 The a-PNA did not show a definite pre-organization, as circular dichroism spectra of single-strands gave rise to very weak signal not showing the exciton coupling pattern; however, when they were bound to complementary achiral PNA they were shown to be prone to form either right-or left-handed helical PNA:PNA double helices. The circular dichroism signature of these complexes can be linked to the propensity of the PNA for either of the two handednesses, 11 and aggregation of cyanine dyes on these PNA:PNA duplexes supported these conclusions. 12 On the other hand, a substituent can be introduced also in the aminoethyl part of the PNA backbone (Fig.…”

mentioning

confidence: 72%

Effect of chirality in gamma-PNA: PNA interaction, another piece in the picture

Manicardi

Corradini

2014

Artificial DNA: PNA & XNA

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“…The CD spectra of open double duplex D and hairpin double duplex E are shown in Figure d. The profiles resemble that observed for PNA:DNA duplexes, indicating similar overall conformational features. Both D and E show a positive CD band at ∼280 nm, with a slight difference in negative band position: 260 nm for the open duplex D and 250 nm for hairpin duplex E .…”

mentioning

confidence: 99%

Structural Design and Synthesis of Bimodal PNA That Simultaneously Binds Two Complementary DNAs To Form Fused Double Duplexes

et al. 2020

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Bimodal PNAs are new PNA constructs designed to bind two different cDNA sequences synchronously to form double duplexes. They are synthesized on solid phase using sequential coupling and click reaction to introduce a second base in each monomer at Cα via alkyltriazole linker. The ternary bimodal PNA:DNA complexes show stability higher than that of individual duplexes. Bimodal PNAs are appropriate to create higher-order fused nucleic acid assemblies.

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Electronic Circular Dichroism of Peptide Nucleic Acids and their Analogues

Cited by 12 publications

References 93 publications

Antibacterial Peptide Nucleic Acids—Facts and Perspectives

Antibacterial Peptide Nucleic Acids—Facts and Perspectives

Effect of chirality in gamma-PNA: PNA interaction, another piece in the picture

Structural Design and Synthesis of Bimodal PNA That Simultaneously Binds Two Complementary DNAs To Form Fused Double Duplexes

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