Electronic and Molecular Properties of an Adsorbed Protein Monolayer Probed by Two-Color Sum-Frequency Generation Spectroscopy

Dreesen, Laurent; Humbert, Christophe; Sartenaer, Yannick; Caudano, Yves; Volcke, Cédric; Mani, A. A.; Péremans, A.; Thiry, P.A.; Hanique, Sophie; Frère, Jean‐Marie

doi:10.1021/la0488001

Cited by 62 publications

(86 citation statements)

References 42 publications

(73 reference statements)

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“…SFG is a coherent process that detects the second-order nonlinear susceptibility of a material. Different components of the susceptibility tensor can be measured by using different polarization combinations of input and output laser beams (40)(41)(42)(43)(44)(45)(46)(47)(48)(49). As shown extensively in the literature (50)(51)(52)(53)(54)(55)(56), different susceptibility components can interfere with each other if some polarization combinations are adopted.…”

Section: Chiral Sfg Vibrational Spectra Of Adsorbed Fibrinogen Obtainmentioning

confidence: 99%

Detection of chiral sum frequency generation vibrational spectra of proteins and peptides at interfaces in situ

Wang

Chen

Clarke

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

186

245

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In this work, we demonstrate the feasibility to collect off-electronic resonance chiral sum frequency generation (SFG) vibrational spectra from interfacial proteins and peptides at the solid͞liquid interface in situ. It is difficult to directly detect a chiral SFG vibrational spectrum from interfacial fibrinogen molecules. By adopting an interference enhancement method, such a chiral SFG vibrational spectrum can be deduced from interference spectra between the normal achiral spectrum and the chiral spectrum. We found that the chiral SFG vibrational spectrum of interfacial fibrinogen was mainly contributed by the ␤-sheet structure. For a ␤-sheet peptide tachyplesin I, which may be quite ordered at the solid͞liquid interface, chiral SFG vibrational spectra can be collected directly. We believe that these chiral signals are mainly contributed by electric dipole contributions, which can dominate the chiroptical responses of uniaxial systems. For the first time, to our knowledge, this work indicates that the off-electronic resonance SFG technique is sensitive enough to collect chiral SFG vibrational spectra of interfacial proteins and peptides, providing more structural information to elucidate interfacial protein and peptide structures. chiral vibrational spectroscopy ͉ interfacial proteins and peptides U nderstanding chirality is important in biology, chemistry, and medicine. For example, chiral surfaces and interfaces are important in asymmetric chemical synthesis, chiral molecule separation, binding between chiral drugs and proteins, crystal growth, and the adsorption of proteins on biomedical material surfaces. Since the excellent work on chiroptical effects using second-harmonic generation (SHG) was presented by Hicks and coworkers (1-4) and Persoons and coworkers (5-7), extensive research has been performed to investigate such effects in oriented thin films or bulk media by using SHG and sum frequency generation (SFG) spectroscopic techniques (8-38). Many excellent experimental demonstrations and theoretical treatments on this topic have been published in the last decade or so. These studies indicate that more structural information about chiral materials can possibly be deduced by SHG and SFG studies. It has been demonstrated that the nonlinear chiral effect detected by nonlinear optical methods, such as SHG and SFG, can be several orders of magnitude larger than those detected by linear optical methods (refs. 8, 29, and 36 and references therein).Currently, several general models have been used to interpret the molecular mechanisms of second-order nonlinear chiral spectra (ref. 36 and references therein). Some experimental results can be interpreted by magnetic-dipole contributions and͞or interference between electric and magnetic-dipole contributions. For other experiments, the factors mentioned above cannot explain the very strong nonlinear chiral signal observed, and interpretations based on a pure electric-dipole contribution have been proposed. Recently, Shen and coworkers (10,17) demonstrated that chir...

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Section: Chiral Sfg Vibrational Spectra Of Adsorbed Fibrinogen Obtainmentioning

confidence: 99%

Detection of chiral sum frequency generation vibrational spectra of proteins and peptides at interfaces in situ

Wang

Chen

Clarke

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

186

245

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“…SFG in such a condition is called DR-SFG. 7,8 DR-SFG spectroscopy has been applied to a number of systems such as a spin-coated dye film on silica, 8 C 60 monolayer on silver, 9 and a colored protein monolayer on platinum 10 with visible probes, and a benzene derivative on gold with UV probes. 11 DR-SFG also allows detection of chirality of thin films.…”

Section: Introductionmentioning

confidence: 99%

Identification of chemical species of fluorescein isothiocyanate isomer–I (FITC) monolayers on platinum by doubly resonant sum‐frequency generation spectroscopy

Maeda¹,

Nagahara²,

Aida³

et al. 2008

J Raman Spectroscopy

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Doubly resonant sum-frequency generation (DR-SFG) spectroscopy of fluorescein-4-isothiocyanate isomer-I (FITC) monolayers on platinum was performed. Vibrational spectra of the monolayers for the IRwavenumber of 1750-1450 cm −1 were measured with visible probes ranging from 431 to 582 nm. Two vibrational bands at 1643 and 1610 cm −1 were observed, and their DR-SFG excitation profiles displayed different shapes. By rinsing the monolayers with an alkaline solution, the smaller wavenumber band disappeared and the larger wavenumber band gained intensity. On the basis of the spectral response to the rinsing, we concluded that the FITC molecules existed on platinum as deprotonated and protonated forms; the former corresponds to the 1643 cm −1 band and the latter to the 1610 cm −1 band. The deprotonated form was assigned to an anionic surface species, and the protonated form to a neutral surface species by comparing the DR-SFG excitation profiles with electronic absorption spectra of the protolytic forms of fluorescein in an aqueous solution (Sjöback R et al., Spectrochimica Acta A 1995; 51: L7-L21). The results demonstrate that the measurement of DR-SFG excitation profiles is a useful technique to identify chemical species of monolayers on metal surfaces.

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“…It has therefore drawn particular attention in the past decade, mainly due to its importance in many biotechnological applications. Biological interactions such as biotin-avidin complexes (Vareiro et al, 2008), or protein A or G (Briand et al 2006;Ha et al, 2007), chemical interactions, like glutaraldehyde or PDITC (1,-4 phenylene diisothiocyanate) linkage (Lee et al, 2006;Raj et al, 2009;Rao et al, 1998;Weiping et al, 1999) and physical interactions, such as simple adsorption (Dreesen et al, 2004), have been studied for antibody immobilization on surfaces. It appears that protein immobilization is strongly dependent on surface properties such as composition, reactivity, wettability, roughness etc (Sethuraman et al, 2004;Xu and Siedlecki, 2007).…”

Section: ! Introductionmentioning

confidence: 99%

Protein pattern transfer for biosensor applications

Volcke

Gandhiraman

Basabe‐Desmonts

et al. 2010

Biosensors and Bioelectronics

Self Cite

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This paper presents a very simple, industrially-scalable method for transferring a highresolution, biologically-active protein pattern from one substrate to another. We demonstrate the transfer of a protein pattern formed initially by microcontact printing from a silicon surface (to which this form of printing is applicable) onto a glass or polymer substrate, almost independently of the surface/bulk properties of the second substrate. A very thin, spin-coated layer of a sugar is used to preserve the structure and organization of proteins during the subsequent plasma deposition of a siloxane polymer, after which the protein pattern could simply be peeled off the silicon substrate and glued onto any other desired substrate.

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Electronic and Molecular Properties of an Adsorbed Protein Monolayer Probed by Two-Color Sum-Frequency Generation Spectroscopy

Cited by 62 publications

References 42 publications

Detection of chiral sum frequency generation vibrational spectra of proteins and peptides at interfaces in situ

Detection of chiral sum frequency generation vibrational spectra of proteins and peptides at interfaces in situ

Identification of chemical species of fluorescein isothiocyanate isomer–I (FITC) monolayers on platinum by doubly resonant sum‐frequency generation spectroscopy

Protein pattern transfer for biosensor applications

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