Electronic Activation of a DNA Nanodevice Using a Multilayer Nanofilm

Abstract

A method to control activation of a DNA nanodevice by supplying a complementary\ud DNA (cDNA) strand from an electro-responsive nanoplatform is reported. To develop\ud functional nanoplatform, hexalayer nanofilm is precisely designed by layer-by-layer\ud assembly technique based on electrostatic interaction with four kinds of materials:\ud Hydrolyzed poly(β-amino ester) can help cDNA release from the film. A cDNA is\ud used as a key building block to activate DNA nanodevice. Reduced graphene oxides\ud (rGOs) and the conductive polymer provide conductivity. In particular, rGOs efficiently\ud incorporate a cDNA in the film via several interactions and act as a barrier. Depending\ud on the types of applied electronic stimuli (reductive and oxidative potentials), a\ud cDNA released from the electrode can quantitatively control the activation of DNA\ud nanodevice. From this report, a new system is successfully demonstrated to precisely\ud control DNA release on demand. By applying more advanced form of DNA-based\ud nanodevices into multilayer system, the electro-responsive nanoplatform will expand\ud the availability of DNA nanotechnology allowing its improved application in areas\ud such as diagnosis, biosensing, bioimaging, and drug delivery

Recent Advances in Sensing Applications of Graphene Assemblies and Their Composites

“…Controlling the number and properties of sheets in multilayered films and the incorporated substances are of fundamental importance for sensor applications. A functional electroresponsive nanoplatform containing hexalayered LbL nanofilm and bilayered rGO barrier layer building blocks was developed for fluorescence turn‐on detection of DNA . Charged layers of poly(β‐amino ester), 5‐carboxyfluorescein (FAM), and Onyx Quencher (OQA) labeled target complementary 32‐mer DNA (cDNA, 5′‐FAM‐ACTCA CTGTGCTGACCAGTCTCTG ACTCG‐OQA‐3′), rGOCOO − , rGONH 3 + , and PEDOT:PSS were deposited onto a gold‐based chip electrode.…”

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

“…Since the distance between core and satellite AuNP is adjustable by adding molecular stimuli (simple DNA strands), two‐layered AuNP–DNA superstructures with two types of same “core” loaded cargos were made . Such a system realized multistage cargo release with programmable degradation rates, because outer‐layer structures prevented the serum factors responsible for assembly degradation from reaching the inner layer.…”

Structural heterogeneity in polymeric nitric oxide donor nanoblended coatings for controlled release behaviors

“…17 Nowadays, materials are expanded to not only polyelectrolytes but also every molecule that has a molecular interaction, such as graphene oxide, 18 drugs, 7,19 nanoparticles, 20 proteins, 21,22 and DNAs. 23 This is the only way to fabricate nanoblended coatings using biomaterials in aqueous solution. Also, by controlling the ionization of polyelectrolytes via adjusting the pH of solutions for weak polyelectrolytes, both the surface and internal structures of coatings can be precisely changed at the nano and micro level.…”