Electron spin resonance spectroscopy, exercise,  and oxidative stress: an ascorbic acid intervention study

Ashton, Tony; Young, Ian S.; Peters, John R.; Jones, Edward; Jackson, Simon K.; Davies, Bruce; Rowlands, Christopher C.

doi:10.1152/jappl.1999.87.6.2032

Cited by 135 publications

(122 citation statements)

References 18 publications

(18 reference statements)

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…There was, however, a mild attenuation of free radical species and LOOH following ingestion of 1 g of ascorbic acid, demonstrating that ascorbic acid does not completely abolish PBN adduct production. These findings are in general agreement with the work of Ashton et al [9] showing a decrease in EPR signal amplitude after supplementation with 1 g ascorbic acid in ten healthy male volunteers. We have previously observed an attenuation of EPR signals following the addition of ascorbic acid to the in vitro oxidation of α-linolenic acid, postulating that ascorbic acid has the ability to scavenge free radicals generated from a lipid environment [30].…”

Section: Discussionsupporting

confidence: 93%

“…The period of 2 h was chosen specifically to allow the venous concentration of ascorbate to rise from baseline to >100 μmol/l to near cell saturation, based on previous work by Ashton et al [9]. The participants were given a small volume of water in order to ensure complete ingestion.…”

Section: Supplementationmentioning

confidence: 99%

“…It is highly versatile, capable of reacting with many aqueous ROS, and forms the first line of antioxidant defence in human plasma exposed to a variety of oxidant insults [8]. However, the benefits of ascorbic acid prophylaxis remain equivocal, with human studies demonstrating an improvement [9], no change [10], or an increase in ROS and subsequent deterioration in vascular function [11,12]. Concerns have been raised about the administration of ascorbic acid in the presence of redoxactive transition metal ions, since ascorbate can reduce transition metal ions and catalyse the Fenton-driven generation of hydroxyl and alkoxyl radicals in vitro [13].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

View full text Add to dashboard Cite

Aims/hypothesis Patients with type 1 diabetes mellitus are more susceptible than healthy individuals to exercise-induced oxidative stress and vascular endothelial dysfunction, which has important implications for the progression of disease. Thus, in the present study, we designed a randomised doubleblind, placebo-controlled trial to test the original hypothesis that oral prophylaxis with vitamin C attenuates rest and exercise-induced free radical-mediated lipid peroxidation in type 1 diabetes mellitus. Methods All data were collected from hospitalised diabetic patients. The electron paramagnetic resonance spectroscopic detection of spin-trapped α-phenyl-tert-butylnitrone (PBN) adducts was combined with the use of supporting markers of lipid peroxidation and non-enzymatic antioxidants to assess exercise-induced oxidative stress in male patients with type 1 diabetes (HbA 1c 7.9±1%, n=12) and healthy controls (HbA 1c 4.6±0.5%, n=14). Following participant randomisation using numbers in a sealed envelope, venous blood samples were obtained at rest, after a maximal exercise challenge and before and 2 h after oral ingestion of 1 g ascorbate or placebo. Participants and lead investigators were blinded to the administration of either placebo or ascorbate treatments. Primary outcome was the difference in changes in free radicals following ascorbate ingestion. Results Six diabetic patients and seven healthy control participants were randomised to each of the placebo and ascorbate groups. Diabetic patients (n=12) exhibited an elevated concentration of PBN adducts (p<0.05 vs healthy, n=14), which were confirmed as secondary, lipid-derived oxygen-centred alkoxyl (RO·) radicals (a nitrogen =1.37 mT and aβ hydrogen =0.18 mT). Lipid hydroperoxides were also selectively elevated and associated with a depression of retinol and lycopene (p<0.05 vs healthy). Vitamin C supplementation increased plasma vitamin C concentration to a similar degree in both groups (p<0.05 vs presupplementation) and attenuated the exercise-induced oxidative stress response (p<0.05 vs healthy).There were no selective treatment differences between groups in the primary outcome variable. Conclusions/interpretation These findings are the first to suggest that oral vitamin C supplementation provides an Diabetologia

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Supplementationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

View full text Add to dashboard Cite

show abstract

“…[16][17][18][19][20][21][22] There are no studies that have been conducted until now about molecular effects of short-term low-intensity exercise training, such as ROS and NO production, especially in patients hospitalized with AMI. There are no reports about this kind of study and our study is the first reporting it.…”

Section: Discussionmentioning

confidence: 99%

“…[16][17][18] Production of ROS in well-trained patients was lower than in sedentary patients, meanwhile, NO was higher. [19][20][21][22][23] It also had been shown that short-term low-intensity exercise training within 5 days could rapidly elevate internal antioxidants in the diaphragm muscle of rats, accompanied by a reduction in contraction-induced lipid peroxidation or ROS production, but generated high NO consistently.…”

mentioning

confidence: 87%

Effect of Short-Term Low-Intensity Exercise Training on Association of Oxygen Free Radicals and Nitric Oxide Production in Patients with Acute Myocardial Infarction

et al. 2013

View full text Add to dashboard Cite

Oxygen, although is highly required for living, can be toxic because it can be easily changed into oxygen free radicals (reactive oxygen species, ROS) and generates oxidative stress. [1][2][3][4][5] This oxidative stress will cause endothelial lesion and will be responded by endothelial cells through nitric oxide (NO) response and immunological response pathway. 6-8 The immunological response begins by increasing cytokines production, activated coagulation factors, and reactivation of platelet in acute thrombotic process. However, acute thrombotic process, which is an initial part of protective mechanism to fix the lesion, often change to become continuous process because of abundance oxidative stress, and then generates various levels of acute coronary syndromes. 3,5 Several studies showed that acute exercise on treadmill test with high intensity Bruce protocol increased oxidative stress because of ROS. 9,10 On the contrary, acute exercise also increased production of NO because of the increment effect of shear stress on endothelial cells. 6,9-15 But, regular exercise Keywords ► acute coronary syndrome ► atherosclerosis ► cardiovascular risk factors ► myocardial infarction ► oxidative stress ► nitric oxide ► cardiac markers ► exercise AbstractModerate-to-high intensity of exercise training within 2 to 3 months decreases oxygen free radicals (reactive oxygen species, ROS) and increases nitric oxide (NO) in outpatients with myocardial infarction. There is no data about the association of ROS and NO after short-term low-intensity exercise training within 5 days in patients hospitalized with acute myocardial infarction (AMI). A total of 32 male patients with AMI were randomized into two groups: 15 patients with short-term low-intensity exercise training within 5 days formed the training group and 17 patients without such exercise training formed the control group. All patients performed exercise treadmill test with modified Bruce protocol before and after the study. F2-isoprostane and NO concentration of the training group increased slightly after modified Bruce exercise treadmill test. Compared with the control group, NO of the training group was also slightly higher. Baseline NO and uric acid were negative predictor variables for F2-isoprostane in all patients hospitalized with AMI, and triglyceride was a positive predictor variable. After the study, physical capacity of the training group was higher; but heart rate and systolic blood pressure were lower significantly. This study showed that short-term low-intensity exercise training for patients hospitalized with AMI did not change ROS and NO productions, but it improved physical capacity and lowered heart rate and systolic blood pressure. NO was negative predictor variable for F2-isoprostane in controlling ROS changes in dynamic compensation mechanism.

show abstract

Vitamin C for preventing and treating pneumonia

Hemilä

Louhiala

2013

Cochrane Database of Systematic Reviews

115

View full text Add to dashboard Cite

Electron spin resonance spectroscopy, exercise, and oxidative stress: an ascorbic acid intervention study

Cited by 135 publications

References 18 publications

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

Effect of Short-Term Low-Intensity Exercise Training on Association of Oxygen Free Radicals and Nitric Oxide Production in Patients with Acute Myocardial Infarction

Vitamin C for preventing and treating pneumonia

Contact Info

Product

Resources

About