Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise

Ashton, Tony; Rowlands, Christopher C.; Jones, Edward; Young, Ian S.; Jackson, Simon K.; Davies, Bruce; Peters, John R.

doi:10.1007/s004210050366

Cited by 173 publications

(125 citation statements)

References 24 publications

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“…This, in combination with the positive association between Δ PBN-adduct and Δ LOOH, strongly suggests that the free radicals detected in the present investigation were lipid-derived. This is in agreement with the work of Ashton et al [31], who also observed a positive correlation between LOOH and PBN adduct concentration at rest (r=0.80, p<0.05) and immediately following exercise using an exercise paradigm identical to that employed in the present study (r=0.71, p<0.05). We suggest that the metal-catalysed reductive decomposition of LOOH may be the precursor of the compound formation of EPR-detectable spin-trapped lipidderived alkoxyl radicals (LOOH±Fe 2+ =RO·).…”

Section: Discussionsupporting

confidence: 94%

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Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

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Aims/hypothesis Patients with type 1 diabetes mellitus are more susceptible than healthy individuals to exercise-induced oxidative stress and vascular endothelial dysfunction, which has important implications for the progression of disease. Thus, in the present study, we designed a randomised doubleblind, placebo-controlled trial to test the original hypothesis that oral prophylaxis with vitamin C attenuates rest and exercise-induced free radical-mediated lipid peroxidation in type 1 diabetes mellitus. Methods All data were collected from hospitalised diabetic patients. The electron paramagnetic resonance spectroscopic detection of spin-trapped α-phenyl-tert-butylnitrone (PBN) adducts was combined with the use of supporting markers of lipid peroxidation and non-enzymatic antioxidants to assess exercise-induced oxidative stress in male patients with type 1 diabetes (HbA 1c 7.9±1%, n=12) and healthy controls (HbA 1c 4.6±0.5%, n=14). Following participant randomisation using numbers in a sealed envelope, venous blood samples were obtained at rest, after a maximal exercise challenge and before and 2 h after oral ingestion of 1 g ascorbate or placebo. Participants and lead investigators were blinded to the administration of either placebo or ascorbate treatments. Primary outcome was the difference in changes in free radicals following ascorbate ingestion. Results Six diabetic patients and seven healthy control participants were randomised to each of the placebo and ascorbate groups. Diabetic patients (n=12) exhibited an elevated concentration of PBN adducts (p<0.05 vs healthy, n=14), which were confirmed as secondary, lipid-derived oxygen-centred alkoxyl (RO·) radicals (a nitrogen =1.37 mT and aβ hydrogen =0.18 mT). Lipid hydroperoxides were also selectively elevated and associated with a depression of retinol and lycopene (p<0.05 vs healthy). Vitamin C supplementation increased plasma vitamin C concentration to a similar degree in both groups (p<0.05 vs presupplementation) and attenuated the exercise-induced oxidative stress response (p<0.05 vs healthy).There were no selective treatment differences between groups in the primary outcome variable. Conclusions/interpretation These findings are the first to suggest that oral vitamin C supplementation provides an Diabetologia

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Section: Discussionsupporting

confidence: 94%

“…It is also feasible that ascorbic acid acted intracellularly as well as in the blood, since cells are saturated at doses of 200 mg [41]. It has been suggested that ascorbic acid is most effective at the aqueous-lipid interface of the cell membrane, scavenging intracellular aqueous radicals [31].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

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“…Marked increases in the amplitude of a spin-trapped radical were seen to coincide with coronary reperfusion. Similar data have also recently been reported from studies of human subjects undertaking strenuous exercise protocols (Ashton et al 1998).…”

Section: Spin Trappingsupporting

confidence: 86%

An overview of methods for assessment of free radical activity in biology

Jackson

1999

Proc. Nutr. Soc.

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fax +44 (0)151 706 5952, email mjj@liv.ac.ukAssays which purport to assess free radical activity in biological systems are multiple. However, despite numerous published descriptions of new methods and modifications of methods to assess free radical activity in biological materials, there is still a lack of reliable techniques for quantification of activity in vivo. Analysis of a number of related indicators and use of a variety of approaches appears the only reliable way to evaluate these processes in vivo. In studies of free radical generation by contracting skeletal muscle we have attempted to use a variety of indicators, including measurement of endogenous antioxidant levels, measurement of indirect indicators of free radical activity (e.g. products of lipid peroxidation, DNA oxidation or protein oxidation) and, where possible, measurement of direct indicators of free radical activity by electron spin resonance techniques. In view of the relative lack of specificity of many available techniques, caution should be exerted in evaluating the numerous examples of isolated single measures of free radical activity which are present in the scientific literature.

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“…There are several reports indicating increased oxidative stress and decreased antioxidant levels during exercise 11,15,[48][49] . The hydroperoxide levels were measured using FOX-1 assay and levels were found in a normal range 27 .…”

Section: Table5mentioning

confidence: 99%

Energy Expenditure and Nutritional Status of Sailors During One Month of Extensive Physical Training

Verma¹,

Kirar²,

Singh³

et al. 2018

Def. Life. Sc. Jl.

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The present study was conducted to determine nutritional requirements during extensive physical training by sailors of Indian Navy. A total of 37 sailors who were undergoing physical trainers course at training establishment of Indian Navy participated in this study. Energy expenditure, energy intakes, nutrient status and body composition changes during one month of training were recorded. Mean energy expenditure was found to be 4035 kcal/day ± 733 kcal/day and an average intake of 4478 kcal/day ± 340 kcal/day with sufficient amount of micro and macronutrients. The level of vitamin and minerals in blood and their excretion were in the normal range. Body composition was also maintained with a marginal decrease in body fat content. Increase in grip strength of passive hand was observed (Basal: 41.5 kg ± 8.8 kg, after 1 month of training: 46.5 kg ± 6.1 kg). Results indicate adequate nutritional support from the diet and positive effects of the training on health.

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Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise

Cited by 173 publications

References 24 publications

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

An overview of methods for assessment of free radical activity in biology

Energy Expenditure and Nutritional Status of Sailors During One Month of Extensive Physical Training

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