Electron spin resonance

Janzen, Edward G.

doi:10.1021/ac60313a033

“…In the last review of electron spin resonance in this Journal (84), suggestions for topics to be discussed in future reviews were solicited. The only response to this request came from two readers who asked why the topic "clinical applications of ESR" was not included in my review.…”

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confidence: 99%

“…In addition, the computer output for the search of all papers listed in Volumes 76 to 79 of Chemical Abstracts having electron spin resonance, ESR, electron paramagnetic resonance or EPR in the title (or in the key words or phrases) was scanned and topics of possible interest were selected for comment. A special attempt was made to continue mentioning papers on those topics selected in my first review (84) which covered Chemical Abstracts, Volume 72 to 75 (1970-71). Also in this review, most of the papers dealing with some aspect of the ESR of radical ions which appeared in Chemical Abstracts, Volume 76 to 79 (1972-73) are listed.…”

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confidence: 99%

Electron spin resonance

Janzen¹

1974

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Born in Manitoba, Canada, he received his BSc and MSc degrees in chemistry at the University of Manitoba in 1957 and 1960 and his PhD degree from Iowa State University in 1963. He joined the chemistry staff at the University of Georgia in 1964 as assistant professor (1964-68) after spending seven months on a postdoctoral appointment at Iowa State University. His research interests are in the area of electron spin resonance applications to studies of the structure and mechani sms of free radicals.

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Discriminative Detection of Biothiols by Electron Paramagnetic Resonance Spectroscopy using a Methanethiosulfonate Trityl Probe

Tan

¹

,

Ji

²

,

Wang

³

et al. 2019

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Biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), coexist in biological systems with diverse biological roles. Thus, analytical techniques that can detect, quantify, and distinguish between multiple biothiols are desirable but challenging. Herein, we demonstrate the simultaneous detection and quantitation of multiple biothiols, including up to three different biothiols in a single sample, using electron paramagnetic resonance (EPR) spectroscopy and a trityl‐radical‐based probe (MTST). We term this technique EPR thiol‐trapping. MTST could trap thiols through its methanethiosulfonate group to form the corresponding disulfide conjugate with an EPR spectrum characteristic of the trapped thiol. MTST was used to investigate effects of l‐buthionine sulfoximine (BSO) and pyrrolidine dithiocarbamate (PDTC) on the efflux of GSH and Cys from HepG2 cells.

show abstract

Discriminative Detection of Biothiols by Electron Paramagnetic Resonance Spectroscopy using a Methanethiosulfonate Trityl Probe

Tan

¹

,

Ji

²

,

Wang

³

et al. 2019

View full text Add to dashboard Cite

Biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), coexist in biological systems with diverse biological roles. Thus, analytical techniques that can detect, quantify, and distinguish between multiple biothiols are desirable but challenging. Herein, we demonstrate the simultaneous detection and quantitation of multiple biothiols, including up to three different biothiols in a single sample, using electron paramagnetic resonance (EPR) spectroscopy and a trityl‐radical‐based probe (MTST). We term this technique EPR thiol‐trapping. MTST could trap thiols through its methanethiosulfonate group to form the corresponding disulfide conjugate with an EPR spectrum characteristic of the trapped thiol. MTST was used to investigate effects of l‐buthionine sulfoximine (BSO) and pyrrolidine dithiocarbamate (PDTC) on the efflux of GSH and Cys from HepG2 cells.

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Electron spin resonance

Cited by 14 publications

References 165 publications

Electron spin resonance

Electron spin resonance

Discriminative Detection of Biothiols by Electron Paramagnetic Resonance Spectroscopy using a Methanethiosulfonate Trityl Probe

Discriminative Detection of Biothiols by Electron Paramagnetic Resonance Spectroscopy using a Methanethiosulfonate Trityl Probe

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