Electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) of multilamellar bodies and multilamellar body-like structures in tannic acid-treated alveolar septal cells.

Ochs, Matthias; Fehrenbach, Heinz; Richter, Joachim

doi:10.1177/42.6.8189041

Cited by 14 publications

(11 citation statements)

References 18 publications

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“…Our microanalytical study using electron spectroscopic imaging [26] indicates that the phospholipid content of the lamellar bodies was not decreased in the human donor lungs studied. The amount of lamellar bodies per cell correlated with the early postoperative oxygenation achieved after SLTx.…”

Section: Dependentmentioning

confidence: 86%

“…Electron spectroscopic imaging (ESI) was used to check whether the phosphorus signal of lamellar bodies, an indicator of relative phospholipid content, was altered [11,26]. Ultrathin sections about 40 nm in thickness were collected on bare 600 hexagonal copper grids.…”

Section: Methodsmentioning

confidence: 99%

“…Ultrathin sections about 40 nm in thickness were collected on bare 600 hexagonal copper grids. Computer-assisted phosphorus imaging was performed by means of a CEM 902 (Zeiss, Oberkochen) according to the three-window method as described previously [26]. This type of electron microscope is equipped with an integrated CastaingHenry energy filter that allows for the selection of inelastically scattered electrons of a given energy loss [8].…”

Section: Methodsmentioning

confidence: 99%

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Ultrastructural pathology of the alveolar type II pneumocytes of human donor lungs

et al. 1998

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Alveolar type II pneumocytes (PII) were studied in 12 human donor lungs perfused with modified Euro-Collins solution during single-lung transplantation (SLTx). While one lung was transplanted, the contralateral donor lung (cDL) was fixed at the time of SLTx for examination by electron microscopy, stereology, and microanalysis. Three groups were then formed: group A (n = 7), cDL without contusions, uneventful early postoperative course; group B (n = 3), cDL with conclusions, uneventful early postoperative course; group C (n = 2), cDL without contusions, early postoperative respiratory dysfunction. The major findings were that the presence of contusions had no effect on PII ultrastructure and that intracellular surfactant-storing lamellar bodies of cDL in group C were characterized by a higher volume-to-surface ratio (VsR) and larger area per cell profile than group A. Correlation analysis based on pooled data (groups A and C) showed that ischaemic time had little effect on PII ultrastructure and bore no relationship to postoperative clinical variables. The duration of preoperative donor intubation had a pronounced influence on ultrastructure and postoperative clinical variables. The stereologically estimated amount of intracellular surfactant and mitochondrial VsR were the only ultrastructural parameters that were significantly associated with early postoperative oxygenation. Lamellar bodies were the only ultrastructural components found to have a significant relationship to postoperative intubation time. The ultrastructural integrity of type II pneumocytes of human donor lungs is an important determinant of early respiratory function following clinical lung transplantation.

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Section: Dependentmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Ultrastructural pathology of the alveolar type II pneumocytes of human donor lungs

et al. 1998

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“…Preservation of surfactant-containing lamellar bodies in alveolar type II cells requires prolonged en bloc staining with half-saturated aqueous uranyl acetate (61,62).…”

Section: Fixing Agents (A)mentioning

confidence: 99%

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Hsia¹,

Hyde²,

Ochs³

et al. 2010

Am J Respir Crit Care Med

779

688

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“…endothelial cells and interstitium, but they pointed out that such LBs were observed only in the lungs fixed with tannic acid, which enhanced the preservation of phos pholipids during processing for electron microscopy. Using the same method of fixation, Ochs et al [1994] compared (by electron spectroscope imaging and electron energy loss spectroscopy) LBs visible in type II pneumocytes with LBlike structures visible in other cells of the alveolar septa. They proved that despite their structural similarity in con ventional transmission electron microscopy the phosphorus signal is much higher in LBs contained within type II epi thelial cells than in LB-like structures (therefore they sug gested that tannic acid could form some artifactual struc tures.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary Surfactant Migrates into the Alveolar Capillaries of Newborn Rats: An Immunoelectron Microscopic Study

Wąsowicz¹,

Kashima²,

Yokoyama³

et al. 1996

Cells Tissues Organs

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The goal of the present study was to elucidate the migration of surfactant-like material into the alveolar capillaries of newborn rats. The lungs of experimental animals were studied by ultrastructural techniques – standard electron microscopy as well as immunoelectron microscopy – using antibodies against synthetic polypeptide complementary to the noncollagenous part of surfactant protein A (SP-A). Antibodies exhibited specific reaction with native rat SP-A in Western blot and immunohistochemical analysis. Surfactant was observed in the alveolar space, type II pneumocytes, macrophages, type I pneumocytes, endothelial cells, the extracellular space of the alveolar septa and in the alveolar capillaries. Lamellar structures observed in all elements of the blood-air barrier as well as in the alveolar capillaries were stained positively with anti-SP-A antibodies. Transport of the pulmonary surfactant into the circulatory system was observed more frequently during the first 6-8 h of extrauterine life of newborn rats, although it was also seen in the blood vessels till 48 h after delivery. The results of our investigation suggest that clearance of surfactant via the circulatory system plays an important role in the early postnatal period.

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Electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) of multilamellar bodies and multilamellar body-like structures in tannic acid-treated alveolar septal cells.

Cited by 14 publications

References 18 publications

Ultrastructural pathology of the alveolar type II pneumocytes of human donor lungs

Ultrastructural pathology of the alveolar type II pneumocytes of human donor lungs

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Pulmonary Surfactant Migrates into the Alveolar Capillaries of Newborn Rats: An Immunoelectron Microscopic Study

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