Electron Microscopy of Viruses of Human Papilloma, Molluscum Contagiosum, and Vaccinia, Including Observations on the Formation of Virus Within the Cell

Melnick, Joseph L.; Bunting, Henry; Banfield, William G.; Strauss, Maurice J.; Gaylord, William H.

doi:10.1111/j.1749-6632.1952.tb39990.x

Cited by 49 publications

(11 citation statements)

References 12 publications

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“…They have been separated by digestion and micromanipulation from the rest of the cell and with the electronmicroscope sometimes appear to be sharply separated from cell cytoplasm. It is doubtful whether, in molluscum, the virus particles develop from the trabeculae of the inclusion matrix, as has been suggested by Melnick et al (25). The histochemical studies of Rake & Blank (120) on sections of molluscum bodies indicated the presence of ribonucleic acid in early inclusions which was compressed by increasing amounts of deoxyribonucleic acid in more mature bodies, a finding which confirms results obtained by microspectrographic methods [Hyden (121)].…”

Section: Pox Virusessupporting

confidence: 60%

“…Excellent reviews of the techniques used, the results obtained, and the difficulties of interpretation have recently been published [Williams (17); Bang (18)]. The pox viruses examined in dried films are variola (19,20), canary pox (21,22), fowlpox (23,24), molluscum (21,23,25), ectromelia (21,23), myxoma (21,26), and fibroma (27). In such preparations all the viruses examined reveal brick-shaped bodies mostly within the size range 250 to 35 0 ffij.lX200 to 250 mj.l.…”

mentioning

confidence: 98%

“…report sequential studies designed to follow the course of infection, and thus, lacking correlation with biological observations, their schemes of developmental cycles must remain conjectural [Schlesinger (40)]. Pox viruses which have been examined in thin sections of infected tissue include fowl pox (3�, 39,41,42), vaccinia (25,38,43,44), molluscum (38,43,45,46), ectromelia (38), myxoma (47), and fibroma (27).…”

mentioning

confidence: 99%

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Pox Viruses

Downie¹,

Dumbell²

1956

Annu. Rev. Microbiol.

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Section: Pox Virusessupporting

confidence: 60%

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confidence: 98%

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confidence: 99%

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Pox Viruses

Downie¹,

Dumbell²

1956

Annu. Rev. Microbiol.

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“…Crystal-like formations have been observed by differential centrifugation of material from human infectious skin papilloma (17,18) as well as in sections of ceils of this lesion (1,19). The intranuclear inclusion bodies of some such cells have been found to contain DNA (20,21) which is acquired during a developmental stage of the inclusion (22,23).…”

Section: Bloch~ Morgan~ Godman Howe and Rosementioning

confidence: 99%

A Correlated Histochemical and Electron Microscopic Study of the Intranuclear Crystalline Aggregates of Adenovirus (Ri-Apc Virus) in Hela Cells

Bloch

Morgan

Godman

et al. 1957

The Journal of Cell Biology

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HeLa cells in tissue cultures infected with types 3, 4, or 7 of adenovirus (RI-APC virus) were studied in order to correlate certain histochemical and electron microscopic findings. Adjacent thin (ca. 0.05 µ) and thick (2–4 µ) sections of osmium-fixed, methacrylate-embedded cells were cut; by mapping the sections the same cells could be identified with both the electron and the light microscope. Intranuclear crystalline aggregates seen with the electron microscope to be composed of ordered arrays of viral particles were found by means of the Feulgen reaction to contain DNA. DNA is therefore assumed to be a constituent of the viral particle. The virus appeared to develop from an osmiophilic Feulgen-negative matrix. Displacement of nuclear chromatin occurred during this process. A Feulgen-azure staining method was found to permit clear distinction between viral and nuclear (host) DNA in thick sections.

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“…It has been observed electron microscopically in thin-sectioned material by Banfield, Bunting, Strauss & Melnick (1951, and Melnick, Bunting, Banfield, Strauss & Gaylord (1952), but their use of formalin fixation and removal of the methacrylate embedding material from their sections are unsatisfactory for the preservation of fine structural detail. Gaylord & Melnick (1953), using more acceptable techniques, also observed the virus, but their published observations were rather cursory and intended only for comparison with their more informative examination of the vaccinia and ectromelia viruses.…”

Section: Introductionmentioning

confidence: 99%

An electron microscope study of molluscum contagiosum

Charles

1960

J. Hyg.

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The development of the molluscum body and of the molluscum contagiosum virus is described. All cells of the molluscum lesion do not form molluscum bodies, but those which do probably show cytoplasmic abnormality prior to the appearance of virus particles within the cytoplasm. Such abnormal cells may reflect the activity of some infective precursor of the morphologically observable virus particle; they may alternatively represent cells which have successfully resisted invasion by the infective precursor.Cells which have failed to resist invasion gradually become filled with virus particles and the nucleus becomes eccentric. The molluscum body thus formed is virtually a bag of virus particles, the wall of the bag being the peripheral cytoplasm, which seems to resist invasion and in which the nucleus remnant can readily be detected.There appear to be two kinds of viruses. Development of the commoner virus is compared and contrasted with studies by Morgan and his colleagues of vaccinia and fowl pox. It is akin to fowl pox in its origin from foci of finely dispersed cytoplasm, here called cytoplasmic clouds; it is akin to vaccinia in that no evidence can be found of a denser, finely granular, pre-nucleoid material; it is like both in that the virus does not observably develop within the nucleus; and it is unlike both in that a nuclear change—the appearance of rather unspecific dense bodies—is seen. The structural changes seen in the virus particles during development are similar to those described by Morgan et al., but a slightly different interpretation is given of the behaviour of the transient ‘nucleoid’: they believe that it expands to form a central viroplasm, whereas in this paper it is believed to disperse through an already present central viroplasm.The second type of virus is of uncertain origin. It may develop from, or at least it seems to be related to, a double-membrane structure seen in abnormal lesion cells.

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Electron Microscopy of Viruses of Human Papilloma, Molluscum Contagiosum, and Vaccinia, Including Observations on the Formation of Virus Within the Cell

Cited by 49 publications

References 12 publications

Pox Viruses

Pox Viruses

A Correlated Histochemical and Electron Microscopic Study of the Intranuclear Crystalline Aggregates of Adenovirus (Ri-Apc Virus) in Hela Cells

An electron microscope study of molluscum contagiosum

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