Electron microscopy of stomatogastric ganglion in the lobster Homarus americanus

Maynard, Edith A.

doi:10.1016/s0040-8166(71)80036-8

Cited by 52 publications

(31 citation statements)

References 13 publications

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“…The gross structure of the STG is similar to that of other decapods, as has been described elsewhere (Maynard, 1971;King, 1976a,b). Figure 1 shows that this ganglion is a flattened oval, with a necklace of cell bodies around the sides and posterior.…”

Section: Gross Structuresupporting

confidence: 59%

“…For instance, the number of neurons in the STG is not clear. The number of cells in the STG of the lobsters Homarus americanus and Panulirus interruptus has been reported to be 27-32 (Maynard, 1971;King, 1976a), but physiological estimates of cell number in the crab C. borealis are lower (Weimann, 1992). Therefore, we serially sectioned and reconstructed six crab STG to determine neuronal cell number.…”

mentioning

confidence: 99%

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Ultrastructure of the stomatogastric ganglion neuropil of the crab, Cancer borealis

Kilman

Marder

1996

J. Comp. Neurol.

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The stomatogastric ganglion (STG) of the crab, Cancer borealis, contains the neural networks responsible for rhythmic pattern generation of the foregut. Neuron counts indicate that the STG of C. borealis has 25-26 neurons, 4-5 fewer than that found in lobsters. We describe the ultrastructural features of the ganglion by focusing on those that may be involved in storage, release, or range of action of peptide modulators, including a lacunar system and multiple types of intercellular junctions. In the neuropil, we identify five synaptic profile classes that contain the invertebrate presynaptic apparatus (dense bars, small clear vesicles), two of which also contain dense core (modulator-containing) vesicles. These latter two are comprised of multiple immunocytochemical classes that are not easily distinguished by structural criteria. In addition, we find neurohemal-like profiles that contain primarily dense core vesicles. Our finding that multiple profile types in the STG possess modulator-containing vesicles coincides with immunocytochemical results better than do previous ultrastructural studies that report only one such profile type. We show that a single modulatory input, stomatogastric nerve axon 1, makes only classical synapses and not neurohemal-like profiles, although some modulators are found in both these profile types. These data provide the groundwork for understanding the architecture of modulatory input-target interactions and suggest ways that the specificity of modulatory effects within a complex neuropil may be attained.

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Section: Gross Structuresupporting

confidence: 59%

mentioning

confidence: 99%

Ultrastructure of the stomatogastric ganglion neuropil of the crab, Cancer borealis

Kilman

Marder

1996

J. Comp. Neurol.

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“…Neurohemal profiles have been reported between the neuropil and the perineural sheath of the STG of P. interruptus (Friend, 1976;King, 1976a) and between the fine and coarse neuropil in C. borealis (Kilman and Marder, 1996). Similar profiles may be present but are not prevalent in H. americanus (Maynard, 1971). In C. destructor, neural profiles containing exclusively dense-core vesicles have not been found within the middle of the STG, but might have been missed, because describing the neuropil of the STG was not the main focus of this study.…”

Section: Ultrastructure Of the Stn And The Stn-son Junctionmentioning

confidence: 94%

“…These profiles are likely to correspond to the profiles described here for C. destructor, which were found along the entire stn, but in contrast to P. interruptus not within the perineural sheath of the STG. Similar to C. destructor, profiles suggestive of neurohemal release sites were found in the anterior part of the stn of H. americanus (Kilman and Marder, 1997), but none were present in the perineural sheath of the STG (Maynard, 1971). Neurohemal profiles have been reported between the neuropil and the perineural sheath of the STG of P. interruptus (Friend, 1976;King, 1976a) and between the fine and coarse neuropil in C. borealis (Kilman and Marder, 1996).…”

Section: Ultrastructure Of the Stn And The Stn-son Junctionmentioning

confidence: 96%

“…A number of studies have examined the ultrastructure of the stn and the STG in the lobster H. americanus (Maynard, 1971), in the spiny lobster P. interruptus (Friend, 1976;King, 1976a,b), and in the crab C. borealis (Coleman et al, 1992;Kilman and Marder, 1996). Only one preliminary study has examined the stn-son junction (Kilman and Marder, 1997).…”

Section: Ultrastructure Of the Stn And The Stn-son Junctionmentioning

confidence: 99%

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Synaptic neuropil in nerves of the crustacean stomatogastric nervous system: An immunocytochemical and electron microscopical study

Skiebe

Ganeshina

2000

J. Comp. Neurol.

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Ontogenetic alteration in peptidergic expression within a stable neuronal population in lobster stomatogastric nervous system

et al. 1998

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In the adult lobster, Homarus gammarus, the stomatogastric ganglion (STG) contains two well-defined motor pattern generating networks that receive numerous modulatory peptidergic inputs from anterior ganglia. We are studying the appearance of extrinsic peptidergic inputs to these networks during ontogenesis. Neuron counts indicate that as early as 20% of development (E20) the STG neuronal population is quantitatively established. By using immunocytochemical detection of 5-bromo-2'-deoxyuridine incorporation, we found no immunopositive cells in the STG by E70. We concluded that the STG neuronal population remains quantitatively stable from mid-embryonic life until adulthood. We then investigated the ontogeny of FLRFamide- and proctolin-like peptides in the stomatogastric nervous system, from their first appearance until adulthood by using whole mount immunocytochemistry. Numerous FLRFamide-like-immunoreactive STG neuropilar ramifications were observable as early as E45 and remain thereafter. From E50 to the first larval stage, one to three STG somata stained, while somatic staining was not observed in larval stage II and subsequent stages. From E50 and thereafter, the STG neuropilar area was immunopositive for proctolin. One to two proctolinergic somata were detected in the STG of the three larval stages but were not seen in embryos, the post-larval stage or in adults. Thus, peptidergic inputs to the STG are present from mid-embryonic life. Moreover, whereas in the adult, STG neurons only contain glutamate or acetylcholine, some neurons transiently express peptidergic phenotypes during development. Although this system expresses an ontogenetic peptidergic plasticity, the STG neurons produce a single stable embryonic-larval motor output (Casasnovas and Meyrand [1995] J. Neurosci. 15:5703-5718).

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Electron microscopy of stomatogastric ganglion in the lobster Homarus americanus

Cited by 52 publications

References 13 publications

Ultrastructure of the stomatogastric ganglion neuropil of the crab, Cancer borealis

Ultrastructure of the stomatogastric ganglion neuropil of the crab, Cancer borealis

Synaptic neuropil in nerves of the crustacean stomatogastric nervous system: An immunocytochemical and electron microscopical study

Ontogenetic alteration in peptidergic expression within a stable neuronal population in lobster stomatogastric nervous system

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