Electron Microscopy of Rhabdovirus‐like Particles in <i>Festuca gigantea</i> with Leaf streak mosaic

Lundsgaard, T.; Albrechtsen, S. E.

doi:10.1111/j.1439-0434.1976.tb01715.x

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…High-throughput sequencing was used successfully to complete the genome sequences of previously reported plant rhabdoviruses where only partial sequence fragments were available, such as Iranian citrus ringspot-associated virus (Sadeghi et al, 2016), ivy vein banding virus (Petrzik, 2012), and soybean blotchy mosaic virus (Lamprecht et al, 2010). Furthermore, HTS could be a key tool to characterize the genomes of some cyto-and nucleorhabdoviruses which have only been characterized biologically, such as broccoli necrotic yellows virus (Lin and Campbell, 1972) and festuca leaf streak virus (Lundsgaard and Albrechtsen, 1976).…”

Section: Rhabdoviridaementioning

confidence: 99%

The Plant Negative-Sense RNA Virosphere: Virus Discovery Through New Eyes

2020

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The use of high-throughput sequencing (HTS) for virus diagnostics, as well as the importance of this technology as a valuable tool for discovery of novel viruses has been extensively investigated. In this review, we consider the application of HTS approaches to uncover novel plant viruses with a focus on the negative-sense, single-stranded RNA virosphere. Plant viruses with negative-sense and ambisense RNA (NSR) genomes belong to several taxonomic families, including Rhabdoviridae, Aspiviridae, Fimoviridae, Tospoviridae, and Phenuiviridae. They include both emergent pathogens that infect a wide range of plant species, and potential endophytes which appear not to induce any visible symptoms. As a consequence of biased sampling based on a narrow focus on crops with disease symptoms, the number of NSR plant viruses identified so far represents only a fraction of this type of viruses present in the virosphere. Detection and molecular characterization of NSR viruses has often been challenging, but the widespread implementation of HTS has facilitated not only the identification but also the characterization of the genomic sequences of at least 70 NSR plant viruses in the last 7 years. Moreover, continuing advances in HTS technologies and bioinformatic pipelines, concomitant with a significant cost reduction has led to its use as a routine method of choice, supporting the foundations of a diverse array of novel applications such as quarantine analysis of traded plant materials and genetic resources, virus detection in insect vectors, analysis of virus communities in individual plants, and assessment of virus evolution through ecogenomics, among others. The insights from these advancements are shedding new light on the extensive diversity of NSR plant viruses and their complex evolution, and provide an essential framework for improved taxonomic classification of plant NSR viruses as part of the realm Riboviria. Thus, HTS-based methods for virus discovery, our 'new eyes,' are unraveling in real time the richness and magnitude of the plant RNA virosphere.

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Section: Rhabdoviridaementioning

confidence: 99%

The Plant Negative-Sense RNA Virosphere: Virus Discovery Through New Eyes

2020

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“…

Several Rhabdoviruses causing diseases of monodiotiledon plants have been reported. See FRANCKI (1973) for a review, GREBER (1972), PLUMB and JAMES (1975), LUNDSGAARD and ALBRECHTSEN (1976).So far Rhabdoviruses have not been reported in the genus his of the Iridaceae family.The description of the morphology and distribution of Rhabdovirus like particles found in the host cells of Iris germanica is the subject of this paper.

Material and Methods/. germanica plants showing yellow stripes and some stunting were found in a garden of Madrid.

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mentioning

confidence: 99%

“…Several Rhabdoviruses causing diseases of monodiotiledon plants have been reported. See FRANCKI (1973) for a review, GREBER (1972), PLUMB and JAMES (1975), LUNDSGAARD and ALBRECHTSEN (1976).…”

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confidence: 99%

A Rhabdovirus in Iris germanica

Rubio-Huertos¹

1978

J Phytopathol

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“…A maximum was obtained at a density of 1.194 g/ml. This value is at the upper limit of the buoyant densities in sucrose of well-characterized rhabdoviruses, namely 1.17 to 1.19 g/ml (Brown et (Lundsgaard & Albrechtsen, 1976, demonstrate that FLSV is a definitive member of the family Rhabdoviridae. As FLSV accumulates in the cytoplasm and possesses only one M protein, it should be placed in subgroup A of plant rhabdoviruses (Matthews, 1982).…”

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confidence: 99%

Physicochemical Characterization of Festuca Leaf Streak Virus

Lundsgaard¹

1987

Journal of General Virology

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SUMMARYParticles of festuca leaf streak virus (FLSV) contain three major proteins. One of these [mol. wt. 49000 (49K)] is the main constituent of the nucleocapsid, whereas the other two (mol. wt. 58K and 20K) were released from the nucleocapsid when particles were treated with non-ionic detergent. The 58K protein is glycosylated. The 58K, 49K and 20K proteins correspond to the G, N and M proteins of rhabdoviruses, respectively. Four minor proteins associated with the virus particles have mol. wt. of 189K, l17K, 101K and 41K. The 189K and 101K proteins are associated with the nucleocapsid, whereas the 117K protein was found in the soluble fraction after detergent treatment. Nucleic acid isolated from virus particles is probably RNA with an estimated mol. wt. of 4.3 x 106. The buoyant density of virus particles in sucrose was estimated to be 1.194 g/ml and the s20.~ to be 704S. The present results, together with previous information, make FLSV a definitive member of subgroup A of the plant rhabdovirus group of the family Rhabdoviridae.Festuca gigantea plants with leaf streaking symptoms contain rhabdovirus-like particles, 330 × 61 nm, that accumulate inside membrane-bound cisternae in the cytoplasm (Lundsgaard & Albrechtsen, 1979). The virus, tentatively named festuca leaf streak virus (FLSV), multiplies in cowpea protoplasts (Van Beek et al., 1985) inoculated with isolated virions and is regarded as a possible member of the family Rhabdoviridae (Matthews, 1982). In its morphology and cytopathology, FLSV resembles northern cereal mosaic virus (NCMV) and barley yellow striate mosaic virus (BYSMV), but no serological cross-reactions were detected when the G protein and the nucleocapsid of FLSV were compared with those of NCMV and BYSMV (Lundsgaard, 1984).In this paper, some of the physicochemical properties of FLSV are compared with those of well-characterized rhabdoviruses.FLSV was purified from infected Festuca gigantea plants grown in a glasshouse. Infected plants were propagated by division. About 50 g of infected leaves were triturated in 5 parts (w/v) TMN buffer pH 8.4 (0.1 M-Tris-HCI, 0.01 M-MgCI> 0.04 M-NazSO3) in a Waring blender. The homogenate was filtered through cheesecloth and then three times through a Celite pad. The virus particles were sedimented by centrifugation at 26000 g for 1 h, resuspended in TMN buffer pH 7.5, and separated by rate zonal centrifugation (linear 10 to 30% sucrose gradient in TMN buffer pH 7.5). The layer with virus particles was removed and centrifuged at 36000 g for 1 h. The pellet was resuspended in water and kept at -20 °C until used. After thawing, precipitated green material was removed by low-speed centrifugation. Electron microscopy of purified virus showed that most of the FLSV particles were in two to four pieces which were linked together.The structural proteins of FLSV were analysed by SDS-PAGE in 5% or 10% horizontal slab gels. Gels and samples were prepared according to Weber & Osborn (1969) as outlined in Sigma Technical Bulletin No. MWS-877. The electrophoresed ...

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Electron Microscopy of Rhabdovirus‐like Particles in Festuca gigantea with Leaf streak mosaic

Cited by 7 publications

References 9 publications

The Plant Negative-Sense RNA Virosphere: Virus Discovery Through New Eyes

The Plant Negative-Sense RNA Virosphere: Virus Discovery Through New Eyes

A Rhabdovirus in Iris germanica

Physicochemical Characterization of Festuca Leaf Streak Virus

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