Electron Microscopy of Intracellular Mineralization of Oral Filamentous Microörganisms in Vitro

Takazoe, I; Kurahashi, Y.; Takuma, S.

doi:10.1177/00220345630420021601

Cited by 24 publications

(8 citation statements)

References 6 publications

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“…The calcification of Strain 13 is in accordance with previous results with this organism (TAKAZOE et al 1963), except that longer periods of incubation had to be used for crystals to occur in appreciable amounts. This difference may partly be explained by the differences in preparation techniques, as trace amounts of calcification may be detected more easily after centrifugation of the cultures.…”

Section: Discussionsupporting

confidence: 90%

Calcification of oral bacteria: an ultra structural study of two strains of Bacterionema matruchotii

Lie

Selvig

1974

European J Oral Sciences

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– The purpose of this study was to test the hypothesis that the pattern of calcification of dental plaque — either intracellularly or extracellularly — is dependent on the type of microorganisms present. Two strains of Bacterionema matruchotii. Strain 13 (Richardson) and Strain 14266 ATCC, were grown in an in vitro system and their calcification studied by transmission electron microscopy. Mineral crystals appeared in the specimens in scattered areas after 2 weeks of incubation, but consistently and in significant amounts after 4 weeks. In Strain 13 the crystals occurred only intracellularly, while in Strain 14266 the crystals were seen both within and around the bacteria. In the Strain 14266 specimens the extracellular crystals were often related to trilaminar vesicles and cell remnants. Differences in potential of calcification between closely related bacterial strains may partly explain the differences observed between calculus formers and non‐formers.

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Section: Discussionsupporting

confidence: 90%

Calcification of oral bacteria: an ultra structural study of two strains of Bacterionema matruchotii

Lie

Selvig

1974

European J Oral Sciences

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“…Mineralization of the cell wall appeared as a late phenomenon in the calcification of Gram-positive rods as previously noted by Takazoe et al (1963) but, by contrast, primary cell wall associated mineralization was frequently seen in cultures of Micrococcus varians, Staphylococcus epidermidis and Neisseria pharyngis.…”

Section: Sidaway Hapsupporting

confidence: 60%

“…Experimental studies of microbial calcification have demonstrated that hydroxyapatite crystals form within and around several species of microorganisms, some of which may be found in calculus (Rizzo et al 1962, Rizzo, Scott & Bladen 1963, Ennever, Vogel & Streckfuss 1971, Ennever, Vogel & Brown 1972, Ennever & Summers 1975, Takazoe, Kurahashi & Takuma 1963. The sequence of calcification observed by Rizzo et al (1963) was that Actinomyces spp., Bacterionema matruchotii and Streptococcus salivarius were calcified primarily intracellularly while with a Veillonella sp.…”

Section: Introductionmentioning

confidence: 99%

A microbiological study of dental calculus

Sidaway

1980

J of Periodontal Research

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Cultures of microorganisms isolated from human calculus have been calcified using a simple in vitro system. All strains had previously produced hydroxyapatite (HAp) under similar experimental conditions. Ultra‐thin sections of specimens embedded in Epon 812 were examined, with a Philips 200 electron microscope, unstained or double‐stained with uranyl acetate and lead citrate. Intracellular crystals of HAp were seen in all strains examined. The majority of Grampositive rods produced only intracellular calcification, usually mesosome associated and extracellular vesicles were rarely seen. In contrast Gram‐negative rods produced extracellular material—vesicles and debris from disrupted cells—which was heavily mineralized. The association of initial intracellular calcification with mesosomes was not always observed. Extracellular calcification was also a feature of some Gram‐positive and all Gram‐negative cocci with cell‐associated deposits apparently originating from the cell wall. A feature of some species, particularly the Gram‐negative cocci, was the presence of randomly arranged spherules of amorphous calcium phosphate within the cytoplasm some of which appeared to be partially converted to HAp. In the majority of microorganisms examined early mineralization was apparently crystalline. It is suggested that extracellular mineralization of Gram‐negative microorganisms may be a significant factor in early matrix calcification in natural calculus.

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“…The calcification of C. matruchotii, like that of many other oral bacteria (Ennever 1960), occurs intracellularly, but electron microscopy of dental plaque during its formation has clearly shown the presence of needle-shaped crystals both within and between bacteria, as well as on their peripheral membrane (Gonzales and Sognnaes 1960;Takazoe et al 1963;Höhling et al 1969;Theilade et al 1976;Lo Storto et al 1990), the predominance of one site or another probably depending on type of bacteria and nutritional factors (Lie and Selvig 1974b).…”

Section: Bacteriamentioning

confidence: 99%

Biological Calcification

2007

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Electron Microscopy of Intracellular Mineralization of Oral Filamentous Microörganisms in Vitro

Cited by 24 publications

References 6 publications

Calcification of oral bacteria: an ultra structural study of two strains of Bacterionema matruchotii

Calcification of oral bacteria: an ultra structural study of two strains of Bacterionema matruchotii

A microbiological study of dental calculus

Biological Calcification

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