Electron Microscopy of Glomeruli in Nephrotoxic Serum Nephritis

Rt, Reid

doi:10.1038/icb.1956.17

Cited by 16 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, although it does no more than demonstrate that an antigen/antibody reaction is a possible explanation of the nephrotic lesions, it should be pointed out that the experimental nephritis produced by nephrotoxic serum (Masugi, 1934) may contain a whole spectrum of lesions from those characteristic of a progressive nephritis to reversible glomerular changes associated with massive proteinuria. Electron microscopic studies of these lesions have been made by Reid (1956) who used small amounts of nephrotoxic serum and they seem to be very similar to the reversible changes reported by Folli et al (1958). If, however, severe lesions are produced by larger doses of nephrotoxic serum the appearances are, as one would expect, quite different (Piel et al, 1955).…”

supporting

confidence: 75%

Discussion on the Importance of Auto-Antibody Disease in Clinical Medicine

1959

Proceedings of the Royal Society of Medicine

View full text Add to dashboard Cite

supporting

confidence: 75%

Discussion on the Importance of Auto-Antibody Disease in Clinical Medicine

1959

Proceedings of the Royal Society of Medicine

View full text Add to dashboard Cite

“…The morphology of serum sickness nephritis resembled in some respects the lesions described for acute human glomerulonephritis (23,24), and for nephrotoxic nephritis (25)(26)(27). In both of these latter entities, there has been described endothelial proliferation, loss of foot processes, and in some instances thickening of the basement membrane.…”

Section: Discussionmentioning

confidence: 61%

Electron Microscopy of Serum Sickness Nephritis

Feldman

1958

The Journal of Experimental Medicine

View full text Add to dashboard Cite

The morphology of antigen-antibody reactions in tissues has been amply documented by studies with the light microscope. At the cytological level, however, the intracellular changes elicited by antigen-antibody union have been poorly defined and vaguely located. As an experimental model to study this problem, serum sickness nephritis was selected first, because there was already a fairly large accumulation of data on the electron microscopic anatomy of the kidney which could be used for comparison (1-17), and second, because specific antigen has been shown to be present in the giomerulus by the fluorescent microscopic technique (18). Serum sickness offered the possibility of studying the cellular events in the development of renal lesions. Materials and MethodsSix albino rabbits, weighing between 2.5 and 3.0 kg., were used. Two were controls; four were injected intravenously with 250 rag. of bovine serum albumin (BSA) 1 per kg. of body weight. The BSA was purified, crystallized, and tagged tightly with p~a. The rate of antigen elimination was followed by determining the disappearance of serum radioactivity. Immune elimination occurred in all four animals 11 days after the injection of antigen; two were killed 1 day later and two were killed 2 days later; i.e., 12 and 13 days after antigen administration.It has been shown that during the period of immune elimination of antigen, the glomerular lesions were most extensive and severe (18), while prior to this period glomerular lesions could not be found by fight microscopy. After antigen was completely lost from the circulation, glomerular lesions were diminished in number and severity. Fluorescence microscopy showed antigen only in those glomerufi which were altered. P.-ESIYLTSThe structure of the normal glomerulus is briefly reviewed here to point out certain salient features for comparison with glomeruli of experimental rabbits.The glomerulus (Figs. 1 and 3) is constructed of a network of patent capillaries which communicate with one another by short, narrow channels.

show abstract

The suppression of experimental proteinuria in the rat by compounds that inhibit increased capillary permeability

Carone

Spector

1960

J. Pathol.

View full text Add to dashboard Cite

EXPERIMENTAL proteinuria may be produced in rats by the injection of anti-rat-kidney serum (Smadel, 1936 ; Heymann and Lund, 1951), the injection of the nucleoside of Puromycin (Frenk et al., 1955; Fiegelson et al., 1957) and the injection of uranium nitrate (Magee and Foreman, 1958). I n all three instances proteinuria is thought to result largely from increased permeability of the glomerulus to plasma protein (Spector, 1954; Goodman and Baxter, 1956; Magee and Foreman, 1958; Harkin and Recant, 1958). METHODSAnti-rat-kidney serum was prepared by a modification of the method of Lippman, Marti and Campbell (1952). Rat kidneys were perfused i n situ via the abdominal aorta with 60 ml. of isotonic saline. The capsule and fat were then removed and the organ homogenised in isotonic saline with a Potter-Elvejhem homogeniser .

show abstract

Electron Microscopy of Glomeruli in Nephrotoxic Serum Nephritis

Cited by 16 publications

References 4 publications

Discussion on the Importance of Auto-Antibody Disease in Clinical Medicine

Discussion on the Importance of Auto-Antibody Disease in Clinical Medicine

Electron Microscopy of Serum Sickness Nephritis

The suppression of experimental proteinuria in the rat by compounds that inhibit increased capillary permeability

Contact Info

Product

Resources

About