Electron-microscopical examination of diffusion and absorption of thoriumdioxide in the cochlea o f the guinea-pig

Ilberg, C. von

doi:10.1007/bf00404154

Cited by 26 publications

(2 citation statements)

References 6 publications

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“…These findings imply that the mesothelial cell layer is open to the passage of large molecules. This has also been shown by the ability of tracers, such as thorium applied to the perilymph, to reach the basal membrane separating the mesothelium from the epithelium [Ilberg, 1968]. The functional and structural barriers across Reissner's membrane are therefore not located in the mesothelium.…”

Section: Increase Of Endolymphatic Pressurementioning

confidence: 96%

Micro-Lesions in Reissner’s Membrane Evoked by Acute Hydrops

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2003

Audiol Neurotol

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In some pathological conditions excessive amounts of endolymph can accumulate and cause swelling, hydrops, of the membranous labyrinth. Reissner’s membrane in the cochlea will distend and may even rupture. We have studied the effects of acute hydrops, followed for up to 5–6 h, in a preparation that allows continuous monitoring of structural alterations in individual cells in Reissner’s membrane. This is accomplished by using laser confocal microscopy on the membrane visualized by labeling its cells with fluorescent dyes. In specimens subjected to hydrops it was observed that discrete structural defects developed in Reissner’s membrane. These were seen as lesions in single cells or in groups of cells in the epithelial layer. It is suggested that through these micro-lesions the electro-chemical environment of the organ of Corti can be altered causing hearing loss and tinnitus during hydrops.

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Section: Increase Of Endolymphatic Pressurementioning

confidence: 96%

Micro-Lesions in Reissner’s Membrane Evoked by Acute Hydrops

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Flock²

2003

Audiol Neurotol

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“…The fact that these odontocete species present the same type of interdental cells as in land mammals, could mean that they would accomplish the same biologic role in 1) anchoring (Hilding, 1952) and secretion (Belanger, 1953;Iurato, 1962;Santi, 1988) of the tectorial membrane, 2) pinocytotic uptaking of substances from the limbus and their transport to endolymph (von Ilberg, 1968) and secretion into tectorial membrane (Voldrich, 1967;Lim, 1970) and 3) moving electrolytes toward endolymph, specially K + (Spicer and Schulte, 1998).…”

Section: Spiral Limbusmentioning

confidence: 99%

Ultrastructural analysis of odontocete cochlea

Morell Ybarz

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The morphological study of the Odontocete organ of Corti including possible pathological features resulting from sound over-exposure, represent a key conservation issue to assess the effects of acoustic pollution on marine ecosystems. Through the collaboration with stranding networks belonging to 26 countries, 150 ears from 13 species of Odontocetes were processed. In this dissertation, we present a standard protocol to 1) compare the ultrastructure of the cochlea in several Odontocete species and 2) investigate possible damage as a consequence of sound exposure, using scanning (SEM) and transmission (TEM) electron microscopy, and immunohistochemistry. In a preliminary study, computerized tomography scans were performed before decalcification with ears of 15 odontocete species, proposing a set of standard measurements which classified very well the species. In addition, the constant ratio between measurements of inner and middle ear structures contributed to confirm the active role of the odontocete middle ear in sound reception mechanism. We established a decalcification protocol using the fast commercial decalcifier RDO® and EDTA (Ethylendiaminetetraacetic acid). Although further experiments should be conducted to assess the suitability of using one or the other method (because the number of samples treated with EDTA was comparatively small), RDO® at specific dilutions decreased the decalcification time of cetacean ear bones with control of the decalcification endpoint, helping a faster access to inner structures. The complementary use of electron microscopy and immunofluorescence allowed the description in odontocetes of new morphological features of tectorial membrane, spiral limbus, spiral ligament, stria vascularis, hair cells and their innervation. Furthermore, this study revealed qualitative and quantitative morphological characteristics of the organ of Corti in high-frequency hearing species, including 1) an outer hair cell (OHC) small length, 2) a thick cuticular plate in OHC, and a thick reticular lamina, 3) robust cup formation of the Deiters cell body, 4) the high development of cytoskeleton in Deiters and pillar cells and 5) the basilar membrane high stiffness. Interestingly, all these features, including a common molecular design of prestin, are also shared by echolocating bats, suggesting a convergent evolution in echolocating species. The presence of scars among hair cell rows, the pattern of stereocilia imprints in the tectorial membrane and the condition of fibrocytes II and IV were criteria suitable to determine or discard possible acoustic trauma, despite the numerous artefacts that rapidly develop as a consequence of tissue autolysis. Consequently, matching the preliminary approximation of the cochlear frequency map with the damaged region would bring information on the sound source that would have triggered a possible lesion.

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The Possible Importance of the Labyrinthine Fluids in the Pathogenesis of Sensorineural Deafness

1970

Novartis Foundation Symposia

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Electron-microscopical examination of diffusion and absorption of thoriumdioxide in the cochlea o f the guinea-pig

Cited by 26 publications

References 6 publications

Micro-Lesions in Reissner’s Membrane Evoked by Acute Hydrops

Micro-Lesions in Reissner’s Membrane Evoked by Acute Hydrops

Ultrastructural analysis of odontocete cochlea

The Possible Importance of the Labyrinthine Fluids in the Pathogenesis of Sensorineural Deafness

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